Determination of the aminotransferase activity in tissues of infected with ectoparasites yearling carp

Abstract

The paper presents data on changes in the activity of amіnotransferase (aspartate aminotransferase – AST, alanine aminotransferase – ALT) in various organs and tissues of carp at different intensity of infestation with ectoparasites. The material for studying the activity of aminotransferases was one-year-old carp, spontaneously invasive with ectoparasites. For this purpose, twelve groups of fish were formed for 6 individuals in each, with a body weight of 38.0 ± 4.8 g. In four groups of fish (control and three experimental) for the defeat by ectoparasites L. cyprinacea, D. vastator and for mixed infestation. Our studies have shown that the activity of aminotransferases in hepatopancreas and skeletal muscles of carp significantly changes in the lesion of fish by lereneae. In particular, the activity of ALT in hepatopancreas was significantly higher compared to the control group. The tendency to increase was observed in tissues of skeletal muscles. In the study of AST activity, its growth in hepatopancreas and skeletal muscle was determined. For the defeat of fish by dactylosurus, the activity of aminotransferases in carp gills varies considerably. In particular, ALT activity in gills of fish of groups 3 and 4 was significantly higher compared to the control group. At the same time, the activity of ALT in the tissues of the hepatopancreas of the 4th experimental group was likely to increase. The tendency to increase is established in tissues of hepatopancreas and gills in the study of activity of AST. As a result of fish invasion by dactylosurus, the activity of AST in hepatopancreas increased by 1.2 times. At the same time, the growth of the AST index in the gills of the first year of carp in the 3rd and 4th groups was established. In the study of the activity of aminotransferases for mixed infestation, it was found that the activity of ALT and AST in tissues of hepatopancreas, skeletal muscles and gills of groups 2, 3 and 4 was significantly higher compared to the control group.

Investigation of internal organs and additive tissue of hybrid hypophthalmichthys (Hypophthalmichthys spp.) as a promising raw material for the production of dietary nutritional products

“…Winter is one of the most difficult periods in fish life, especially for the young, in the first year of life [24]. With the lack of feeding, the effective consumption of natural foods by annual herbivorous fish is almost stopped in late August or the first decade of September.…”

The influence of the drugs “Brovermectin-granulate TM” and “Avesstim TM” on indicators of non-specific resistance of one year-old carp fish infested with monogeneans

“…Loboyko Yu. V. and others (Lobojko, 2012;Loboiko et al, 2017) established that the complex use of the drug "Brovermectin-granulate TM " and the immunomodulator "Avesstim TM " for carp lerneosis promotes the activation of the immune system, and the acceleration of the recovery of damaged tissues and cells, the improvement of hemodynamics, metabolism and the general state of the body. In view, we studied the influence of the drugs "Brovermectin-granulate TM " and "Avesstim TM " on the indicators of non-specific resistance of one-year-old white carp and crucian carp, infested with dactylogyrus and hyrodactylus, and scaly carp infested with diplozoans.…”

State of the organism of the same year scaly carp infected by Eudiplozoon nipponicum

“…Ключові слова: однорічки коропа лускатого, Eudiplozoon nipponicum, інтенсивність та екстенсивність інвазії, кров, гематологічні показники, білковий склад крові, природна резистентність, пероксидне окиснення ліпідів, антиоксидантна система. Вступ В останнє десятиліття антропогенної діяльності (безконтрольне ввезення посадкового матеріалу, ущільнені посадки риб, надмірне забруднення водойм органічними та мінеральними добривами) у вирощувальних ставах значно змінився склад паразитофауни. Зокрема, з'явилися нові інвазійні хвороби, а відомі захворювання почали проявлятись вже в інших формах, збільшилася частка моногенеозів (дактилогіроз, гіродактильоз, диплозооноз) тощо (Martyshuk et al, 2016;Loboiko et al, 2017;Grynevych, 2018;Goncharov, 2019). Одним із найменш вивчених моногеноїдозів, на сьогоднішній день, є диплозооноз коропа.…”