Determination of telmisartan in rat tissues by in-tube solid-phase microextraction coupled to high performance liquid chromatography

Abstract

A poly(methacrylic acid-ethylene glycol dimethacrylate, MAA-EGDMA) monolithic capillary was used for the direct and on-line extraction of telmisartan from Sprague-Dawley rat tissue (heart, kidney, and liver) homogenates. Under optimized conditions, the tissue homogenates were simply diluted with a mixture of phosphate buffer (pH 2)/ACN (90:8 v/v), and then injected for extraction only after centrifugation and filtration. Coupled to HPLC with fluorescence detection, the method was linear over the range of 1.25-1500 ng/g for telmisartan in heart and kidney, 12.5-15 000 ng/g in liver with correlation coefficients over 0.9992. The detection limits were found to be in the range from 0.24 to 1.8 ng/g. RSDs for intra- and inter-day ranged from 1.2 to 8.1%. The determination of telmisartan in treated rat tissues was achieved by using the proposed method.

Monolith as a new sample preparation material: Recent devices and applications

“…This strategy was first applied to the extraction of phenols in an aqueous sample by Svec and co‐workers 49. Extraction using a monolith was first carried out on the basis of this approach, and many articles were published thereafter 49, 53–56, 61, 72–107. The published applications are listed in Tables 2–5.…”

Porous polymer monoliths for extraction: Diverse applications and platforms

“…The small-diameter monolithic SPE column had to be bypassed during the separation on the analytical column so that the flow rate could be increased to 0.5 mL/min. This configuration has been used for a range of applications including the determination of methylxanthines in human serum [82], ketamine [83] and amphetamine class drugs [84] in urine samples, camptothecin in human plasma [85], angiotensin II receptor antagonists for the treatment of hypertension [86,87] and antibiotics in chicken eggs [88]. One of the specific advantages of this material for these kinds of analysis is that the biocompatibility of the polymer means that is does not irreversibly bind proteins and thus does not foul easily.…”

Simultaneous determination of 5-hydroxyindoles and catechols from urine using polymer monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection

“…The monolithic material exhibited a high extraction capacity as well as benign biocompatibility [45,46].…”