Determination of taurine metabolism by measurement of 15N-enriched taurine in cat urine by gas chromatography—mass spectrometry

Stämpfli, Andreas; Ballèvre, Olivier; Fay, Laurent B.

doi:10.1016/0378-4347(93)80488-p

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…The N-pentafluorobenzoyl di-n-butylamine derivative was prepared by a modification of the procedure described by Kataoka et al (16) as described previously (11). Briefly, an aliquot of 100 L of urine (hydrolyzed or not) was diluted in 750 L of water.…”

Section: Derivatizationmentioning

confidence: 99%

Evaluation of Taurine Metabolism in Cats by Dual Stable Isotope Analysis

Fay

Métairon

Montigon

et al. 1998

Analytical Biochemistry

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Section: Derivatizationmentioning

confidence: 99%

Evaluation of Taurine Metabolism in Cats by Dual Stable Isotope Analysis

Fay

Métairon

Montigon

et al. 1998

Analytical Biochemistry

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“…Several analytical methods are available, but most of them are time‐consuming or not accurate for quantification . Some of these techniques include thin‐layer chromatography (TLC) , capillary electrophoresis (CE) , spectrometry , spectrofluorometry , enzymatic , microbiological assay , gas chromatography (GC) and high‐performance liquid chromatography (HPLC) …”

Section: Introductionmentioning

confidence: 99%

“…Spectrometry and CE techniques are not widely used in vitamin determination owing to their low sensitivities. GC is not widely used for the determination of vitamins due to the limited volatility of many of the vitamins as well as their sensitivity to hot GC injectors and certain types of stationary phases. This raises the need for sample pre‐treatment with derivatization agents prior to analysis, thus, making the analysis time consuming and the vitamins prone to sample degradation due to the high temperatures that are involved in the separation processes.…”

Section: Introductionmentioning

confidence: 99%

Quantification of eight water soluble vitamins in Sutherlandia frutescens species from Botswana using a validated reversed phase HPLC method

Abibu

Takuwa

Sichilongo

2019

Separation Science Plus

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A simple and rapid-reversed phase high-performance liquid chromatography method for the separation and quantification of water-soluble vitamins; B 1 , B 2 , B 3 , B 4 , B 6 , B 9 , B 12 and C from Sutherlandia frutescens (a traditionally used medicinal plant found in Botswana), is presented. Vitamins were extracted using ultrasonic assisted extraction while clean-up was done by solid-phase extraction with silica-based C-18 cartridges.Eight vitamins were separated and identified using an Agilent 1260 Infinity HPLC system fitted with a diode array detector. Two different types of silica-based bonded C18 columns of equal length and internal diameter were compared for their separation efficiencies using a methanol/formic acid mobile phase system at flow rates of 0.5 and 1.0 mL/min on a gradient elution programme. The separation of eight water-soluble vitamins was achieved in 12 min at 266 nm. Analytical performance charactristics were evaluated for percent recovery, precision as percent relative standard deviation, limits of detection, limits of quantification and linearity for each vitamin. The method developed herein was applied to determine some water soluble vitamins in a Sutherlandia frutescens plant from Botswana.

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“…A number of analytical methods have been developed for vitamins determination, such as volumetric assays [ 13 , 14 ], microbiological assays [ 15 ], spectrometric assays [ 16 , 17 ], capillary electrophoresis (CE) [ 18 ], gas chromatography (GC) [ 19 ], high-performance liquid chromatography (HPLC) with different detection like Ultraviolet–visible spectroscopy (UV-vis) with variable wavelength or diode array detection (DAD) [ 7 , 20 – 25 ], fluorescence detection (FLD) [ 26 ], Electrochemical detection (ED) [ 27 , 28 ], and HPLC/mass spectrometry (LC/MS) [ 16 , 29 ]. Among the above analytical techniques, HPLC methods attract increased attention due to its simplicity, high sensitivity and wide dynamic range.…”

Section: Introductionmentioning

confidence: 99%

A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection

et al. 2018

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A new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL-1, respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.

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Determination of taurine metabolism by measurement of 15N-enriched taurine in cat urine by gas chromatography—mass spectrometry

Cited by 11 publications

References 17 publications

Evaluation of Taurine Metabolism in Cats by Dual Stable Isotope Analysis

Evaluation of Taurine Metabolism in Cats by Dual Stable Isotope Analysis

Quantification of eight water soluble vitamins in Sutherlandia frutescens species from Botswana using a validated reversed phase HPLC method

A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection

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