“…A number of analytical methods have been developed for vitamins determination, such as volumetric assays [ 13 , 14 ], microbiological assays [ 15 ], spectrometric assays [ 16 , 17 ], capillary electrophoresis (CE) [ 18 ], gas chromatography (GC) [ 19 ], high-performance liquid chromatography (HPLC) with different detection like Ultraviolet–visible spectroscopy (UV-vis) with variable wavelength or diode array detection (DAD) [ 7 , 20 – 25 ], fluorescence detection (FLD) [ 26 ], Electrochemical detection (ED) [ 27 , 28 ], and HPLC/mass spectrometry (LC/MS) [ 16 , 29 ]. Among the above analytical techniques, HPLC methods attract increased attention due to its simplicity, high sensitivity and wide dynamic range.…”