A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection

Abstract

A new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL-1, respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.

Development of liquid chromatography-mass spectrometry method to determine biotin content in nutritional products and supplements

“…Referring to paper [4] for sample processing, samples are thoroughly homogenized before treatment step. Then, the sample was weighed an appropriate amount into a 50 mL centrifuge tube, added water to a volume of 25 mL (for solid samples), shaked for some minutes.…”

“…To evaluate vitamin H content in nutritional products and supplements, it is required to develop feasible analytical methods. In the world, there have been a number of published studies on biotin content determination methods including: high performance liquid chromatography combined with different detectors such as: UV [3,8], fluorescence with pre-column derivatization [4], mass spectroscopy [1-2 , 5, 6, 7]. Due to the poor absorption in the UV region and the inability of autoflourescense, these methods are not appreciated for the selectivity as well as the time saving of the derivatization processes.…”

Research progress on the detection of water-soluble vitamins in food using liquid chromatography

“…; Long-term high-dose supplementation of pyridoxine can lead to sensory neuropathy [9,10] Vitamin B7 (VB7) Involved in fatty acid synthesis, gluconeogenesis, and branched-chain amino acid catabolism Deficiency can lead to seborrheic dermatitis, loss of appetite, fatigue, etc. [11,12] Vitamin B9 (VB9)…”