Research progress on the detection of water-soluble vitamins in food using liquid chromatography

Abstract

Currently, liquid chromatography is widely used for the analysis and determination of water-soluble vitamins (WSVs) in functional foods. However, due to the diversity of sample characteristics and the requirement for purification accuracy, the chromatographic conditions and specific methods used vary. This paper reviews the chromatographic conditions of liquid chromatography techniques for detecting WSVs in food, as well as the selection of liquid chromatography methods in different application fields. The chromatographic conditions include chromatographic columns, column temperature, mobile phase, flow rate, and elution gradient, and the application scope of different chromatographic conditions is compared. Liquid chromatography methods include high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry, and discussions are made on the applications and prospects of these two methods under different chromatographic conditions. Therefore, this review provides research insights into different chromatographic conditions and methods for food quality inspection and biochemical analysis research.