Determination of tadalafil in small volumes of plasma by high-performance liquid chromatography with UV detection

Cheng, Chien‐Chuan; Chou, Chen Hsi

doi:10.1016/j.jchromb.2005.06.017

Cited by 38 publications

(22 citation statements)

References 10 publications

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“…The two-step extraction processes were sufficient to isolate the tadalafil and loratadine from plasma without any interfering endogenous peaks at 225 nm. The method is specific and more sensitive than the other reported methods [4,9]. No interference with constituent from the plasma matrix was observed.…”

Section: Stock Solution Stabilitymentioning

confidence: 73%

“…To date, no simple, sensitive and rapid high performance liquid chromatographic method with commonly used ultraviolet absorbance detection has been reported for the tadalafil quantitation at therapeutic concentration in human plasma or serum. HPLC-UV method for determination of tadalafil in rat plasma has been reported [9]. The calibration range was 10-2000 ng/mL and the run time was 18 min.…”

Section: Introductionmentioning

confidence: 99%

“…We tried to develop LC-MS method for the estimation of tadalafil using ion trap mass analyzer, but could not succeed due to poor detectability of drug. Tadalafil contains conjugated system and exhibit intensive UV absorption around 220, 280 and 290 nm [9]. So, we developed a simple, rapid, sensitive and selective HPLC-UV method for the quantitation of tadalafil in human plasma using liquid-liquid extraction.…”

Section: Introductionmentioning

confidence: 99%

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Validated liquid chromatographic–ultraviolet method for the quantitation of tadalafil in human plasma using liquid–liquid extraction

Shakya

Awwad²,

Arafat³

et al. 2007

Journal of Chromatography B

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Section: Stock Solution Stabilitymentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Validated liquid chromatographic–ultraviolet method for the quantitation of tadalafil in human plasma using liquid–liquid extraction

Shakya

Awwad²,

Arafat³

et al. 2007

Journal of Chromatography B

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“…This solution was sonicated for 10 min and centrifuged at 14,000×g for 10 min at +4°C. The supernatant was then transferred to tubes and tested for tadalafil purity by comparing its absorbance spectra with that published by Cheng and Chou (2005).…”

Section: Tadalafil Solutionmentioning

confidence: 99%

Influence of the PDE5 inhibitor tadalafil on redox status and antioxidant defense system in C2C12 skeletal muscle cells

Duranti

Ceci

Sgrò

et al. 2017

Cell Stress and Chaperones

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Phosphodiesterase type 5 inhibitors (PDE5Is), widely known for their beneficial effects onto male erectile dysfunction, seem to exert favorable effects onto metabolism as well. Tadalafil exposure increases oxidative metabolism of C2C12 skeletal muscle cells. A rise in fatty acid (FA) metabolism, requiring more oxygen, could induce a larger reactive oxygen species (ROS) release as a byproduct thus leading to a redox imbalance. The aim of this study was to determine how PDE5I tadalafil influences redox status in skeletal muscle cells to match the increasing oxidative metabolism. To this purpose, differentiated C2C12 skeletal muscle cells were treated with tadalafil and analyzed for total antioxidant capacity (TAC) and glutathione levels as marker of redox status; enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) engaged in antioxidant defense; and lipid peroxidation (TBARS) and protein carbonyls (PrCar) as markers of oxidative damage. Tadalafil increased total intracellular glutathione (tGSH), CAT, SOD, and GPx enzymatic activities while no changes were found in TAC. A perturbation of redox status, as showed by the decrease in the ratio between reduced/oxidized glutathione (GSH/GSSG), was observed. Nevertheless, it did not cause any change in TBARS and PrCar levels probably due to the enhancement in the antioxidant enzymatic network. Taken together, these data indicate that tadalafil, besides improving oxidative metabolism, may be beneficial to skeletal muscle cells by enhancing the enzymatic antioxidant system capacity.

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“…[4][5][6][7] Owing to these pharmacological properties, the interest in developing a new formulation of and generic tadalafil for ED treatment has increased. [8] Thus far, several chromatographic methods have been developed for tadalafil determination in plasma, including liquid chromatography (LC) coupled with ultraviolet (UV) [9,10] and fluorescence detection. [11] In recent pharmacokinetic studies, LC-tandem mass spectrometry (LC-MS/MS) has primarily been used for measuring tadalafil in plasma because of its high sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Validated UPLC-MS/MS method for the determination of tadalafil in human plasma and its application to a pharmacokinetic study

Kim

Nam

Kwon

et al. 2017

Transl Clin Pharmacol

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A simple, rapid, and reliable UPLC-MS/MS method was developed and validated for the determination of tadalafil in human plasma. The plasma samples were deproteinized with acetonitrile. Chromatographic separation was performed on a Shiseido C18 (100 × 2.1 mm, 2.7 μm) column with isocratic elution using 2.0 mM ammonium acetate and acetonitrile (55:45, v/v) with 0.1% formic acid at a flow rate of 0.7 mL/min. The total run time was 1 min per sample. The quantitative analysis was performed using multiple reaction monitoring at transition of m/z 390.4 → 268.3 for tadalafil and m/z 475.3 → 283.3 for sildenafil as an internal standard. The method was fully validated over a concentration range of 5-1,000 ng/mL with a lower quantification limit of 5 ng/mL. Intra-and inter-day precision (relative standard deviation, %RSD) were within 8.4% and accuracy (relative error, %RE) was lower than -3.2%. The developed and validated method was successfully applied to a pharmacokinetic study of tadalafil (20 mg) in Korean healthy male subjects (n = 12).

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Determination of tadalafil in small volumes of plasma by high-performance liquid chromatography with UV detection

Cited by 38 publications

References 10 publications

Validated liquid chromatographic–ultraviolet method for the quantitation of tadalafil in human plasma using liquid–liquid extraction

Validated liquid chromatographic–ultraviolet method for the quantitation of tadalafil in human plasma using liquid–liquid extraction

Influence of the PDE5 inhibitor tadalafil on redox status and antioxidant defense system in C2C12 skeletal muscle cells

Validated UPLC-MS/MS method for the determination of tadalafil in human plasma and its application to a pharmacokinetic study

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