Validated liquid chromatographic–ultraviolet method for the quantitation of tadalafil in human plasma using liquid–liquid extraction

Detection and Validated Quantification of the Phosphodiesterase Type 5 Inhibitors Sildenafil, Vardenafil, Tadalafil, and 2 of Their Metabolites in Human Blood Plasma by LC-MS/MS─Application to Forensic and Therapeutic Drug Monitoring Cases

“…Methods for the determination of PDE-5 inhibitors have been described using liquid chromatography (LC) coupled with different detectors such as UV, 5,6 diode array, 7 chemiluminescence, 8 fluorescence, or (tandem) mass spectrometers. Gas chromatographic mass-spectrometric techniques have also been used for this purpose.…”

Spectrophotometric determination of tadalafil in pure and dosage forms

“…These methods do not involve procedural steps and do not take more operator time and exper- tise like HPLC and other methods. In terms of simplicity, rapidity, sensitivity, expense and free from interference by common additives and excipients, the methods could be considered superior in comparison with the previously reported methods, especially with those based on chromatography [3][4][5][6][7] or other reported spectrophotometric methods [9][10][11]. The reagents utilized in the proposed methods are cheaper, readily available and the procedures do not involve any critical reaction conditions or tedious sample preparation.…”

“…Extensive literature survey revealed that the determination of TDF in pure and dosage forms are not official in any pharmacopoeia and therefore, require much more investigation. Several analytical methods that have been reported for the estimation of TDF in biological fluids or pharmaceutical dosage forms include liquid chromatography [3][4][5][6][7], densitometry [8] and spectrophotometry [9][10][11].…”

Validated UPLC-MS/MS method for the determination of tadalafil in human plasma and its application to a pharmacokinetic study

“…[4][5][6][7] Owing to these pharmacological properties, the interest in developing a new formulation of and generic tadalafil for ED treatment has increased. [8] Thus far, several chromatographic methods have been developed for tadalafil determination in plasma, including liquid chromatography (LC) coupled with ultraviolet (UV) [9,10] and fluorescence detection. [11] In recent pharmacokinetic studies, LC-tandem mass spectrometry (LC-MS/MS) has primarily been used for measuring tadalafil in plasma because of its high sensitivity and selectivity.…”