Determination of Surface Accessibility of the Cellulose Substrate According to Enzyme Sorption

Podgorbunskikh, Ekaterina; Бычков, А. Л.; Lomovsky, O. I.

doi:10.3390/polym11071201

Cited by 22 publications

(10 citation statements)

References 15 publications

(19 reference statements)

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“…The enzyme-cellulose substrate interactions at crystalline region of cellulose were vigorously studied. It was discovered that the enzymes adsorb onto cellulosic surfaces yeilding productive hydrolysis (Baig 2016a;Podgorbunskikh et al 2019). The penetrating action of cellulose-binding modules of cellulases depends on their size, concentration, and binding equilibrium.…”

Section: Interactions Between Enzyme and Materials Surfaces At The Molmentioning

confidence: 99%

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Baig

2020

Bioresour. Bioprocess.

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For the production of biofuel (bioethanol), enzymatic adsorption onto a lignocellulosic biomass surface is a prior condition for the enzymatic hydrolysis process to occur. Lignocellulosic substances are mainly composed of cellulose, hemicellulose and lignin. The polysaccharide matrix (cellulose and hemicellulose) is capable of producing bioethanol. Therefore, lignin is removed or its concentration is reduced from the adsorption substrates by pretreatments. Selected enzymes are used for the production of reducing sugars from cellulosic materials, which in turn are converted to bioethanol. Adsorption of enzymes onto the substrate surface is a complicated process. A large number of research have been performed on the adsorption process, but little has been done to understand the mechanism of adsorption process. This article reviews the mechanisms of adsorption of enzymes onto the biomass surfaces. A conceptual adsorption mechanism is presented which will fill the gaps in literature and help researchers and industry to use adsorption more efficiently. The process of enzymatic adsorption starts with the reciprocal interplay of enzymes and substrates and ends with the establishment of molecular and cellular binding. The kinetics of an enzymatic reaction is almost the same as that of a characteristic chemical catalytic reaction. The influencing factors discussed in detail are: surface characteristics of the participating materials, the environmental factors, such as the associated flow conditions, temperature, concentration, etc. Pretreatment of lignocellulosic materials and optimum range of shear force and temperature for getting better results of adsorption are recommended.

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Section: Interactions Between Enzyme and Materials Surfaces At The Molmentioning

confidence: 99%

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Baig

2020

Bioresour. Bioprocess.

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“…It is assumed that the surface of solids is energetically homogeneous (adsorption centers are the same), but lignocellulose biomass is a macroporous material, which leads to measurement error [14]. However, even with modifications taken into account, gas desorption methods cannot correctly estimate the surface area available for enzymes [15]. In particular, the catalytic core of Trichoderma reesei endoglucanase has a size of about 4-5 nm, which makes the surface of pores with a smaller diameter inaccessible [16].…”

Section: Introductionmentioning

confidence: 99%

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

et al. 2020

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The surface area is an important parameter in setting any biorefining technology. The aim of this study was to investigate the applicability of sorption of methylene blue to characterize the surface of the main biomass carbohydrates: α-cellulose, sigmacell cellulose, natural gum, β-glucan, and starch. The morphology of particles of the model objects was studied by scanning electron microscopy. Nitrogen adsorption isotherms demonstrate that the selected carbohydrates are macroporous adsorbents. The monolayer capacities, the energy constants of the Brunauer–Emmett–Teller (BET) equation, and specific surface areas were calculated using the BET theory, the comparative method proposed by Gregg and Sing, and the Harkins–Jura method. The method of methylene blue sorption onto biomass carbohydrates was adapted and mastered. It was demonstrated that sorption of methylene blue proceeds successfully in ethanol, thus facilitating surface characterization for carbohydrates that are either soluble in water or regain water. It was found that the methylene blue sorption values correlate with specific surface area determined by nitrogen adsorption/desorption and calculated from the granulometric data. As a result of electrostatic attraction, the presence of ion-exchanged groups on the analyte surface has a stronger effect on binding of methylene blue than the surface area does. Sorption of methylene blue can be used in addition to gas adsorption/desorption to assess the accessibility of carbohydrate surface for binding large molecules.

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“…The resulting monomers and their concentration in hydrolysates are affected strongly by the original chemical composition of biomass particles and the pretreatment temperature (Russ et al 2016). Additionally, removing hemicelluloses at high temperatures can effectively increase cellulose digestibility (Zhao et al 2012) and enhanced cellulose accessibility for enzymes provides high concentration of monomers due to dramatic changes in biomass morphology after pretreatment aimed at enhancing the reactivity of biomass (Podgorbunskikh et al 2019).…”

Section: Hplc Chromatograms Of Stexp Samples Of Biomassmentioning

confidence: 99%

Perspective pretreatment method of beech and poplar wood and wheat straw in 2G biofuel production processing

Pažitný

Russ²,

Boháček

et al. 2020

NBC

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Monosaccharides such as glucose, xylose and arabinose are the main monomer units of which cellulose and hemicelluloses are composed. The cellulose and hemicelluloses content in many biomass species makes them suitable for 2G bioethanol production. Today, when 1G bioethanol production is closely monitored due to its enormous consumption of food raw materials such as wheat or corn grains, larger companies are gradually moving to pilot operations of 2G bioethanol production. However, cellulose and hemicelluloses contained in biomass are only very slightly accessible to enzymes used in 2G bioethanol production. Therefore pretreatment methods such as steam explosion are very suitable to use for fractionation of cell structure. In this paper, we tested the cellulose accessibility. We compared the cellulose accessibility of wheat straw particles with wooden particles obtained from beech and poplar. Particle size was less than 0.7 mm. We identified the optimal conditions of steam explosion pretreatment at reaction temperature of 200 °C for wheat straw, poplar and beech wood particles. The main indicator of accessibility was concentration of monomers obtained from enzymatic hydrolysis. The concentration of monomer was determined by high performance liquid chromatography. The experimental results showed different accessibility measure for each type of biomass species.

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Determination of Surface Accessibility of the Cellulose Substrate According to Enzyme Sorption

Cited by 22 publications

References 15 publications

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Interaction of enzymes with lignocellulosic materials: causes, mechanism and influencing factors

Sorption of Methylene Blue for Studying the Specific Surface Properties of Biomass Carbohydrates

Perspective pretreatment method of beech and poplar wood and wheat straw in 2G biofuel production processing

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