Perspective pretreatment method of beech and poplar wood and wheat straw in 2G biofuel production processing

Abstract

Monosaccharides such as glucose, xylose and arabinose are the main monomer units of which cellulose and hemicelluloses are composed. The cellulose and hemicelluloses content in many biomass species makes them suitable for 2G bioethanol production. Today, when 1G bioethanol production is closely monitored due to its enormous consumption of food raw materials such as wheat or corn grains, larger companies are gradually moving to pilot operations of 2G bioethanol production. However, cellulose and hemicelluloses contained in biomass are only very slightly accessible to enzymes used in 2G bioethanol production. Therefore pretreatment methods such as steam explosion are very suitable to use for fractionation of cell structure. In this paper, we tested the cellulose accessibility. We compared the cellulose accessibility of wheat straw particles with wooden particles obtained from beech and poplar. Particle size was less than 0.7 mm. We identified the optimal conditions of steam explosion pretreatment at reaction temperature of 200 °C for wheat straw, poplar and beech wood particles. The main indicator of accessibility was concentration of monomers obtained from enzymatic hydrolysis. The concentration of monomer was determined by high performance liquid chromatography. The experimental results showed different accessibility measure for each type of biomass species.

New approaches in pretreatment methods of beech particles in enzymatic hydrolysis – effect of impregnation time and reactor selection

“…HPLC analysis showed that the monosaccharides in obtained hydrolysates had a different elution time (Table 1). The elution time did not depend on concentration of hydrolyzed monosaccharides however, it depended on set-up and conditions of HPLC method (Pažitný et al 2020b). inhibition products ranged from 4.4 g.L -1 to 4.9 g.L -1 among all the hydrolysates obtained from pretreated beech particles.…”

“…Today it often replaces fossil fuels used in heating plants producing heat and hot water for households (Plešingerová et al 2020;Nosek et al 2021). In recent years, however, attention has focused not only on the production of biopellets and other fuel products based on dendromass, but also on research into the disintegration of dendromass through its pretreatment and hydrolysis, especially enzymatic hydrolysis (Wang et al 2013;Pažitný et al 2020a and2020b). Dendromass-based hydrolysates can then be used in the alcoholic fermentation process.…”

“…In addition, information obtained during enzymatic hydrolysis process and also from the material composition optimization can be potentially used in enzymology and in the wood processing industry (Pažitný 2019). The composition of different lignocellulosic materials may vary considerably between each other type (Jørgensen et al 2007;Joshi et al 2011;Bertero et al 2012;Hazuchová et al 2017;Kucharska et al 2018;Pažitný et al 2020b) and the chemical composition of individual wood species is variable also. The wood composition can also depend on whether wood is softwood or hardwood (Prasad et al 2007;Iqbal et al 2013;Shahzadi et al 2014;Pažitný et al 2020b).…”

“…The composition of different lignocellulosic materials may vary considerably between each other type (Jørgensen et al 2007;Joshi et al 2011;Bertero et al 2012;Hazuchová et al 2017;Kucharska et al 2018;Pažitný et al 2020b) and the chemical composition of individual wood species is variable also. The wood composition can also depend on whether wood is softwood or hardwood (Prasad et al 2007;Iqbal et al 2013;Shahzadi et al 2014;Pažitný et al 2020b). However, wood is essentially composed of lignin and hemicelluloses (Côté 1968), which are tightly crosslinked and form a complex structure.…”