Determination of <sup>58</sup>Co‐Vitamin B<sub>12</sub> Absorption in Pernicious Anemia by Use of Whole‐body Counting: Reproducibility and Control of Gut Transit Time

Hjelt, Karsten; Rasmussen, Per Attrup; Munck, O.

doi:10.1111/j.0954-6820.1977.tb15675.x

Cited by 7 publications

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References 18 publications

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“…Hinton et al (6) showed that the transit time for radiopaque pellets and powdered carmine correlated well. We have shown a good correlation between the passage of nonabsorbed J8Co-B12 and the pellets (8). In agreement with these results, our present investigation suggests that the carmine and the isotopes administered have similar transit times.…”

Section: Counting Staiisticssupporting

confidence: 91%

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Hjelt

Munck

Hippe

et al. 1977

Journal of Internal Medicine

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In 19 control patients and 10 patients with pernicious anemia (PA), the vitamin B12 (B12) absorption was determined simultaneously with whole‐body counting (FRB12) and with a double isotope technique employing incomplete stool collection (FAB12). The test dose was administered orally and consisted of 8 ml of a 10 ml solution containing 0.5 μg of 58Co‐B12 (approximately 0.4 μCi) and 2 mg of 51CrCI3 (approximately 20 μCi). Two ml of the solution were used as standard. In order to follow the passage of the inabsorbable tracer, 51CrCI3, the patients were given 25 radiopaque pellets and 4 carmine tablets (2 g) to swallow immediately after the test dose. Counting of a 3–4 ml specimen from one of the first two red stools was sufficient for calculating FAB12. The findings correlated closely with the FRB12 values (r=0.99, N=39, p<0.001). In the control subjects, the FAB12 averaged 74% (range 37–88). In the patients with PA given intrinsic factor (IF), the FAB12 averaged 40% (range 22–59). When IF was not given, FAB12 averaged 2% (range 0–9). The test, therefore, is a valid indicator of pernicious anemia. Reproducibility: For the mean value of 74%, the standard deviation (S.D.) was 5%, corresponding to a coefficient of variation (CV) of 7%. In the patients not given IF, S.D. was 1% and CV 50%, and in those given IF, S.D. was 8% and CV 20%. This shows the test to be as reliable as whole‐body counting. Carmine tablets proved to be a good indicator of isotope‐containing stool. The test is easy to perform and requires only a scintillation well‐counter. No co‐operation on the part of the patients is necessary. The test is also suitable for outpatients. Furthermore, it is independent of kidney function and flushing with B12 is avoided.

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Section: Counting Staiisticssupporting

confidence: 91%

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Hjelt

Munck

Hippe

et al. 1977

Journal of Internal Medicine

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Dependent proximal loop colostomy: Does it defunction the distal colon?

Schofield

Cade

Lambert

1980

Journal of British Surgery

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The operation of dependent proximal loop colostomy is described. This colostomy avoids the complications of prolapse and paracolostomy hernia and is easy to close. It appears clinically to totally defunction the distal colon; his ability was studied by means of a radioactive tracer. Ten patients were given chromium-51 by mouth and it was found that none of this spilled over into the distal loop. It is concluded that dependent proximal loop colostomies effectively defunction the distal colon.

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Direct and quantitative vitamin B12 absorption measurement in patients with disorders in the distal part of the bowel

et al. 1994

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Direct and quantitative vitamin B12 absorption studies were performed in 25 patients with disorders in the distal small intestine using whole body counting as the gold standard. Simultaneously, vitamin B12 absorption was also determined by the more simple stool spot test (SST) which incorporates 51CrCl3 as a nonabsorbable marker. The SST provided a reliable direct and quantitative measure of vitamin B12 absorption in patients with previous ileal resections due to Crohn's disease (CD) (n = 7) as compared with whole body counting. In ulcerative colitis (UC) patients with either an ileal pelvic reservoir (n = 10) or a conventional ileostomy (n = 8), markedly shorter bowel transit times and absence of colon may have hindered sufficient mixture of the tracer and marker isotopes which could explain the false absorption values according to the SST in single patients. Therefore, an intact colon and a near-normal bowel transit time seem to be essential for performance of the SST. Whole body counting showed, as expected, that all CD patients except one had decreased vitamin B12 absorption (median 23%; range 3-39%) (normally > 35%). In UC patients with ileostomy, only one had a markedly decreased vitamin B12 absorption, two borderline normal values, while the rest had normal values (median 54%, range 15-76%). All UC patients with ileal pelvic reservoir had normal vitamin B12 absorption values (median 40.5%, range 36-87%). We conclude that vitamin B12 substitution therapy is probably required in patients with CD with ileal resection.(ABSTRACT TRUNCATED AT 250 WORDS)

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Determination of ⁵⁸Co‐Vitamin B₁₂ Absorption in Pernicious Anemia by Use of Whole‐body Counting: Reproducibility and Control of Gut Transit Time

Cited by 7 publications

References 18 publications

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Dependent proximal loop colostomy: Does it defunction the distal colon?

Direct and quantitative vitamin B12 absorption measurement in patients with disorders in the distal part of the bowel

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Determination of 58Co‐Vitamin B12 Absorption in Pernicious Anemia by Use of Whole‐body Counting: Reproducibility and Control of Gut Transit Time

Cited by 7 publications

References 18 publications

Vitamin B12 Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Vitamin B12 Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Dependent proximal loop colostomy: Does it defunction the distal colon?

Direct and quantitative vitamin B12 absorption measurement in patients with disorders in the distal part of the bowel

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Product

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Determination of ⁵⁸Co‐Vitamin B₁₂ Absorption in Pernicious Anemia by Use of Whole‐body Counting: Reproducibility and Control of Gut Transit Time

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection

Vitamin B₁₂ Absorption Determined with a Double Isotope Technique Employing Incomplete Stool Collection