Determination of ¹⁵N enrichment of taurine in cat urine by high resolution fast‐atom bombardment mass spectrometry

Abstract

A method is described for measuring the stable isotopic enrichment of taurine in cat urine samples by high resolution fast-atom bombardment mass spectrometry, after 15N labelled taurine was given to cats for the purpose of investigating taurine metabolism. The 15N enrichment of taurine was measured after hydrolysis and purification of taurine by anion/cation exchange chromatography. The isotopic ratio of taurine was determined by measuring the [M+H]+ ion peaks in the spectra of the unlabelled and labelled compounds under multiple ion scan conditions. The overall standard deviation of the measurement is better than 4%. This method requires no derivation and uses only 500 microL of urine samples.

Separation methods for taurine analysis in biological samples

“…With mass spectrometry may be another reason for varying results between (MS) detection, urine sample could be hydrolyzed laboratories. Fortunately, this could be standardized with 12 mol / l hydrochloric acid and then purified by by taking blood samples between 08.00 and 09.00 h anion / cation-exchange chromatography [11]. If a after overnight fasting.…”

“…Taurine without extra reagent pumps, mixing manifolds and could be washed out with water and good recoveries reaction coils, etc. So, pre-column derivatization could also be obtained [5,11]. Unfortunately, extra HPLC methods are more often applied when analyz- [5] ing taurine in complex biological samples.…”