Determination of Sulfonamide Residues in Food by Micellar Liquid Chromatography

Abstract

This paper presents new methods of determination of sulfonamide residues in food products of animal origin, based on liquid chromatography with a micellar mobile phase. The methods employ a technique of direct injection of the sample and preliminary isolation of the analyte by extraction in the liquid-solid and liquid-liquid system. The methods have been characterized by providing the parameters of the calibration curves, the range of linearity, limit of detection, and precision and accuracy of particular determinations. The recovery of the sulfonamides introduced into the food products studied has also been determined.

LC of sulfonamide residues in poultry muscle and eggs extracts using fluorescence pre‐column derivatization and monolithic silica column

“…A number of such methods report simultaneous or single assay procedures for sulfa drugs in chicken muscle and/or eggs, employing techniques such as enzyme immunoassays [5][6][7], cyclic voltammetry and flow injection coupled to HPLC [8], gas chromatography [9,10], gas chromatography-mass spectrometry [11,12], micellar LC [13,14], hydrophilic interaction chromatography-mass spectrometry [15] and RP-HPLC .…”

Validation of Micellar LC-Based Methods Applied to Analyze Foodstuffs

“…Another advantage is the use of micellar solutions as the solvent in a matrix, as hydrophobic compounds are also solubilized due to their interactions with the organic environment inside the micelle. This is useful in complex samples such as foodstuffs [3][4][5][6][7][8]11,[14][15][16][17][18][19][20][21][23][24][25][26][27]. Thus, extraction is avoided, expediting the experimental procedure, improving the reproducibility and decreasing the amount of organic solvent.…”

“…Thus, extraction is avoided, expediting the experimental procedure, improving the reproducibility and decreasing the amount of organic solvent. MLC-based methods mainly use UVvisible absorbance [3,4,[6][7][8]12,13,15,[18][19][20][21][22][23][24][25][26], fluorescence [11,14] or electrochemistry [5] detection. MLC cannot be coupled to MS because the surfactant and the buffer salt easily dirty the detector; therefore, the use of this expensive instrumentation is avoided.…”

“…These mobile phases are appropriate for both hydrophobic and hydrophilic analytes, thereby covering a wide range of compounds, as nutrients [3][4][5][6][7][8], drugs [9][10][11][12][13][14][15][16], additives [17,18] and toxics [19][20][21][22][23][24][25][26][27]. The conditions can even be optimized to separate different analytes in the same run [4][5][6][10][11][12][13][14][15][17][18][19]24,27]. Another advantage is the use of micellar solutions as the solvent in a matrix, as hydrophobic compounds are also solubilized due to their interactions with the organic environment inside the micelle.…”

Micellar high performance liquid chromatographic determination of flunixin meglumine in bulk, pharmaceutical dosage forms, bovine liver and kidney

“…Micellar mobile phases usually need less quantity of organic modifier and generate less amount of toxic waste in comparison to aqueous-organic solvents, so that they are less toxic, non-inflammable, biodegradable and relatively inexpensive [18]. MLC has proved to be a useful technique in the determination of diverse [19][20][21][22][23][24], including food samples [25][26][27][28].…”