Determination of Sulfamethazine and Trimethoprim in Liquid Feed Premixes by HPLC and Diode Array Detection, with an Analysis of the Uncertainty of the Analytical Results

Abstract

Sulfamethazine (SMZ) and trimethoprim (TMP) are antibacterials used in veterinary practice. This paper describes a method for their determination in veterinary liquid feed premixes that is based on liquid chromatography with diode array detection. Gradient elution with methanol and ammonium acetate achieved excellent separation of the two analytes within 15 min without any interference from the matrix. Absorbance of the column effluent was monitored at 264 nm for SMZ and at 230 nm for TMP. Detailed analyses of the uncertainties of determinations afford estimated expanded uncertainties of, respectively, 0.2 and 0.1 w/v % for typical SMZ and TMP concentrations of 10.7 and 2.1 w/v %, respectively. At the lower end of the calibrated range of the method, the dominant source of uncertainty is the preparation of standards and the construction of the calibration line.

Arene–Arene Coupled Disulfamethazines (or Sulfadiazine)-Phenanthroline-Metal(II) Complexes were Synthesized by In Situ Reactions and Inhibited the Growth and Development of Triple-Negative Breast Cancer through the Synergistic Effect of Antiangiogenesis, Anti-Inflammation, Pro-Apoptosis, and Cuproptosis

“…47,48 Sulfamethazine is an antimicrobial sulfonamide, which is mainly used to treat various infections of livestock. 49 Phenanthroline has a large π-bond and a rigid planar structure, which is often used in the design of metal complex drugs to enhance the activity of metal-based complexes. 38(a) In this study, using sulfamethazine, ophenanthroline, copper perchlorate hexahydrate, cobalt acetate tetrahydrate, and nickel chloride hexahydrate as raw materials, Cu(II)-based complex HA-Cu, Co(II)-based complex HA-Co, and Ni(II)-based complex HA-Ni were synthesized by in situ reaction.…”

Eco-friendly multivariate curve resolution-alternating least squares and chromatographic quantifications of some veterinary drug residues in pharmaceutical industrial wastewater

“…S1 † ) is 4-amino- N -(4,6-dimethyl-2-pyrimidine)benzene sulfonamide, is also called sulfamethazine (SMZ). 14 It is a broad-spectrum antibacterial drug, belongs to the sulfonamide class. It is widely used in veterinary practice to treat livestock diseases such as gastrointestinal and respiratory tract infections.…”

“…It is widely used in veterinary practice to treat livestock diseases such as gastrointestinal and respiratory tract infections. 14 Various methods were reported for SDD determination, such as LC-MS/MS, 15 HPLC. 16,17 …”

Sulphamethazine derivatives as immunomodulating agents: New therapeutic strategies for inflammatory diseases

“…It is known to inhibit the synthesis of dihydrofolic acid. Mostly this drug have been used for the treatment of various infections in livestock, such as gastrointestinal and respiratory tract infections [10]. Since the amide is an important functional group present in a number of drugs, the synthesis of amide linkages has special significance in organic synthesis, and in pharmaceuticals industries [11].…”