“…Clonidine has been in clinical use for over 40 years, which is used to treat hypertensive disorders, hyperactivity disorder, anxiety disorders, migraine, menopausal flushing and certain pain conditions (Neil, 2011). Published methods have been used for the determination of clonidine, including HPLC (Rosansky et al, 1993), LC-MS/MS (Zhao et al, 2006;Muller et al, 2007;Parekh et al, 2008;Ghosh et al, 2009;Li et al, 2011), GC (Velasquez et al, 1983;Nazarali et al, 1986;Chu et al, 1979), GC-MS (Arrendale et al, 1988;Girault et al, 1988;Yamahata et al, 1994) and CE (Hercegová, 1998;Filipic et al, 2013). Hence a transdermal patch formulation of clonidine was developed (MacGregor et al,1985) to maintain clonidine plasma concentration in a steady state over a prolonged period of time and reduce the risk of adverse effects.…”