Determination of subfractions of amniotic fluid alpha-fetoprotein in diagnosing spina bifida and congenital nephrosis.

Ruoslahti, Erkki; Pekkala, Aulikki; Comings, David E.; Seppålå, M

doi:10.1136/bmj.2.6193.768-a

Cited by 31 publications

(11 citation statements)

References 2 publications

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“…They observed differences in proportions of Con A variants in the amniotic fluid of fetuses with neural tube defects and in the amniotic fluid of fetuses without malformations. This finding has already been confirmed by Brock [4], by Hindersson et al [6] and by Ruoslahti et al [15]. Using Con A affinity-chromatography, Dambuyant et al [5] and Ruoslahti et al [J4] were able to demonstrate that the proportion of AFP variants is different in sera of hepatoma patients and of patients with yolk sac tumours, in our recent study we confirmed the results obtained 0300-9475/81/0700-0015S02.00 © 1981 Blackweil Scientific Publications by previous workers but we found that the estimation of Con A AFP variants was not satisfactory to distinguish AFP present in sera of hepatoma patients from AFP present in sera of patients with benign liver disorders and in sera of patients with liver metastases of cancer.…”

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confidence: 58%

Microheterogeneity of Alpha‐fetoprotein in Patient Serum as Demonstrated by Lectin Affino‐electrophoresis

Bręborowicz¹,

Maćkiewicz²,

Breborowicz³

1981

Scand J Immunol

152

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Microheterogeneity of alpha-fetoprotein (AFP) present in the sera of 76 patients was studied by lectin affino-immunoelectrophoresis. Seventeen patients had benign liver disorders and the remaining 59 patients were treated for primary or secondary liver cancer or yolk sac tumour. By means of Con A, AFP was divided into two variants, while lentil lectin (LCA) made it possible to separate AFP in three variants. In some patients the relative concentrations of Con A and LCA AFP variants were similar; these patients were believed to produce AFP of the same 'profile'. Fourteen AFP profiles were observed by estimation of the area enclosed by precipitates corresponding to respective AFP variants. It was also possible to estimate the AFP profile on the basis of a simple visual analysis of the electrophoretic plates. The obtained results indicate that the AFP profiles of patients with cancer were variable. In spite of variations of the AFP profile in cancer patients, in most cases it was possible to differentiate primary liver cancer from yolk sac tumour and from liver metastases of cancer. In addition, in two-thirds of hepatoma patients the AFP profile was different from the profile observed in patients with benign liver disorders.

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confidence: 58%

Microheterogeneity of Alpha‐fetoprotein in Patient Serum as Demonstrated by Lectin Affino‐electrophoresis

Bręborowicz¹,

Maćkiewicz²,

Breborowicz³

1981

Scand J Immunol

152

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“…The AFP present in the sera of patients No. 1 to 3 had a concanavalin A-binding pattern similar to the AFP present in fetal serum (Ruoslahti et al, 1978) while the AFP in the sera of patients No. 4 to 7 was similar to AFP present in amniotic fluid in this respect.…”

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confidence: 90%

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confidence: 99%

“…Human AFP exhibits concanavalin A-binding heterogeneity (Smith and Kelleher, 1973;Ruoslahti et al, 1978). The relative amounts of the AFP present in human amniotic fluid and in fetal serum which do not react with concanavalin A are 15 to 40 per cent and less than 10 per cent respectively (Ruoslahti et al, 1978). This difference in the pattern of molecular heterogeneity of AFP present in fetal serum and amniotic fluid has recently been exploited to improve the precision of analysis of amniotic fluid AFP in the diagnosis of neural tube defects Kelleher et af, 1980).…”

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confidence: 99%

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Origin of Amniocentesis‐induced Rises of Alpha‐fetoprotein Concentrations in Maternal Serum

Dallaire

Bélanger²,

Smith³

et al. 1980

BJOG

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“…Affinity chromatography using Con A bound to sepharose was performed according to the method of Smith et al 6 with certain modifications.…”

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confidence: 99%

Affinity Chromatography of Amniotic Fluid Alphafetoprotein Using Concanavalin A-Sepharose

Noyes¹,

Wood

1981

Ann Clin Biochem

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SUMMARYWe measured Concanavalin A (Con A) non-binding alphafetoprotein in amniotic fluid from 21 normal pregnancies and 20 abnormal pregnancies (complicated by anencephaly, spina bifida, or exomphalos) using small Con A-sepharose chromatography columns. There was a highly significant difference between percentage non-binding alphafetoprotein levels for pregnancies with a normal outcome (mean result 32 %; range 18--47 %) and pregnancies complicated by a neural tube defect or exomphalos (mean 14%; range 7-20%). The test is therefore of potential value in cases where there is uncertainty over the interpretation of total amniotic fluid alphafetoprotein levels.The measurement of alphafetoprotein (AFP) in amniotic fluid is now widely used as a follow-up to serum AFP screening for the detection of neural tube defects such as spina bifida and anencephaly. When a cut-off level of five standard deviations above the mean for normal pregnancies is taken, amniotic fluid AFP estimations give a false-positive rate of 0·2% but may miss 10% of open spina bifida cases.' Thus there is a need for additional diagnostic procedures which would aid the interpretation of 'borderline' amniotic fluid AFP results, for example, those cases that fall between 2· 5 and 5 standard deviations above the mean.AFP, a glycoprotein containing approximately 4 % carbohydrate, is composed of two molecular variants which differ only in their carbohydrate content.s Affinity chromatography using Concanavalin A (Con A) will separate amniotic fluid AFP into two components: Can A non-binding AFP, which passes through the chromatography column, and a Con A binding fraction, which is retained.The proportion of Con A non-binding AFP in fetal serum and amniotic fluid: 2-6 % of fetal serum AFP and 14-45% of amniotic fluid AFP is not bound by Con A. 3

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Determination of subfractions of amniotic fluid alpha-fetoprotein in diagnosing spina bifida and congenital nephrosis.

Cited by 31 publications

References 2 publications

Microheterogeneity of Alpha‐fetoprotein in Patient Serum as Demonstrated by Lectin Affino‐electrophoresis

Microheterogeneity of Alpha‐fetoprotein in Patient Serum as Demonstrated by Lectin Affino‐electrophoresis

Origin of Amniocentesis‐induced Rises of Alpha‐fetoprotein Concentrations in Maternal Serum

Affinity Chromatography of Amniotic Fluid Alphafetoprotein Using Concanavalin A-Sepharose

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