Origin of Amniocentesis‐induced Rises of Alpha‐fetoprotein Concentrations in Maternal Serum

Dallaire, Louis; Bélanger, Luc; Smith, Catharine L.; Kelleher, P C

doi:10.1111/j.1471-0528.1980.tb04436.x

Cited by 8 publications

(4 citation statements)

References 13 publications

(12 reference statements)

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“…After amniocentesis, up to 20% of patients have evidence of fetomaternal hemorrhage by the KB test or increases in alpha fetoprotein levels (Hay et al , 1979; Dallaire et al , 1980; MacLennan et al , 1994). Samura and colleagues (2003) measured the amount of cff DNA in maternal circulation after amniocentesis and found that it increased significantly in 79% of post-procedure maternal samples.…”

Section: Introductionmentioning

confidence: 99%

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

et al. 2010

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Objective Cell-free fetal DNA (cffDNA) in maternal plasma results from degradation of fetal and/or placental cells. Our objective was to determine if chorionic villus sampling (CVS) causes increased release of fetal and/or maternal DNA. Methods Fifty-two pregnant women were recruited prior to CVS, performed for clinical indications, at 10 5/7 to 13 2/7 weeks. Maternal blood was collected before and within 15 minutes after CVS. cffDNA was extracted from plasma. Real-time polymerase chain reaction (PCR) amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the Y chromosome sequence DYS1 were used as measures of total and fetal DNA, respectively. All samples were analyzed in triplicate without knowledge of fetal gender. Results Sensitivity of DYS1 detection in male fetuses was 100% (n=30); specificity in female fetuses was 100% (n=22). While a majority of women had >50% post-procedure increases in both fetal and total DNA, some showed post-procedure decreases. However, overall median proportional increases were not statistically significant. Gestational age (GA), placental location, and individual CVS operator did not correlate with changes in DNA levels. Conclusions While there were no statistically significant overall changes in DNA levels after CVS, as-yet undiscovered variables may influence the extent of post-procedure release of cell-free DNA in the circulation of pregnant women.

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Section: Introductionmentioning

confidence: 99%

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

et al. 2010

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“…In amniotic fluid, this fraction declines from about 20 to 10 per cent of the total AFP between 15 and 20 weeks of gestation, whereas in second-and third-trimester fetal serum percentages between 0.5 and 5 per cent have been established (Ruoslahti et al, 1978;Toftager-Larsen et al, 1980;Toftager-Larsen, 1990). Differences in non-Con A-reactive AFP levels in both sources of MSAFP have been used to investigate the contribution of each source to the maternal AFP pool (Toftager-Larsen, 1986;Kelleher and Smith, 1979;Dallaire et al, 1980). We believe raised MSAFP levels in second-trimester oligohydramnios to originate from amniotic fluid: a defect in the fetal membranes before 16 weeks of gestation allows leakage of amniotic fluid to the uterine cavity and resorption in the maternal decidual circulation.…”

Section: Introductionmentioning

confidence: 99%

Origin of raised maternal serum alpha‐fetoprotein levels in second‐trimester oligohydramnios

et al. 1992

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Concanavalin A (Con A) subtyping of alpha-fetoprotein (AFP) revealed higher concentrations of AFP non-reactive with Con A in sera of 12 pregnant women with second-trimester oligohydramnios and raised total serum AFP levels than in sera of 42 pregnant women with raised total serum AFP levels and a normal amniotic fluid volume. This suggests that in oligohydramnios the origin of excess AFP in the maternal compartment is amniotic fluid. It is proposed that oligohydramnios and the associated raised maternal serum AFP levels are caused by damage of the fetal membranes prior to 16 weeks of gestation resulting in leakage of amniotic fluid to the decidual tissue and resorption in the maternal circulation.

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“…On the basis of our data, kidney function seems to contribute to the elimination of troponins. After amniocentesis, 5-20% of patients have evidence of fetal-maternal hemorrhage as indicated by increases in maternal serum ␣-fetoprotein (1)(2)(3)(4)(5) or by the BetkeKleihauer test (6 -8 ). The Betke-Kleihauer test can differentiate fetal from maternal erythrocytes by the relative resistance of hemoglobin F-containing cells to acid elution, and it is the most popular method of diagnosing and assessing the severity of fetal-maternal hemorrhage (9 ).…”

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confidence: 99%

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

et al. 2003

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Origin of Amniocentesis‐induced Rises of Alpha‐fetoprotein Concentrations in Maternal Serum

Cited by 8 publications

References 13 publications

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

Origin of raised maternal serum alpha‐fetoprotein levels in second‐trimester oligohydramnios

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

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