Determination of styrene oxide adducts in DNA and DNA components

Schräder, Wolfgang; Linscheid, Michael

doi:10.1016/0021-9673(95)00558-9

Cited by 36 publications

(40 citation statements)

References 17 publications

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“…Since CE-ESI-MS was first applied for analysis of DNA adducts in 1994 by Janning et al [75], numerous studies have been conducted to detect modified oligonucleotides and modified nucleotides. The researches that have performed on determination of modified oligonucleotides included styrene oxide-modified oligonucleotides [75,82], alkylated oligonucleotides and oligonucleotides containing halogenated nucleobases [79], N-(acetylamino) fluorine-modified oligonucleotides [83], and unmodified oligonucleotides [77]. The modified nucleotides that have been quantitatively studied included N 7 , O-6, N 2 -modified guanosine monophosphate [82],…”

Section: Dna Adducts Damaged Dna and 8-hydroxydeoxyguanosinementioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Section: Dna Adducts Damaged Dna and 8-hydroxydeoxyguanosinementioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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“…After reaction of styrene oxide with DNA and the digestion into oligonucleotides using the above described procedure, styrene oxide mono-adducts were detected in di-, tri-, and tetranucleotides, whereas pentanucleotides exhibit monoand discernible amounts of di-adducts. Hexanucleotides were generally modified twice [77]. Results also indicated that styrene oxide did not exhibit sequence specificity, but showed strong preference for purines versus pyrimidines in in vitro experiments.…”

Section: Dna Adductsmentioning

confidence: 84%

“…The presence of covalently modified oligonucleotide analytes was verified by full scan MS analysis in the negative ion mode, but the alkylation site of styrene oxide at the nucleobase could not be deduced this way. In subsequent work, on-line sample stacking was employed to enhance sensitivity of the assay [77,78]. Using reaction products of styrene oxide with mononucleotides, fragments were obtained under CID giving some indication about the reaction sites in the molecules.…”

Section: Dna Adductsmentioning

confidence: 99%

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

et al. 2005

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This review is focused on the capillary electrophoresis-mass spectrometric (CE-MS) analysis of nucleic acid constituents in the broadest sense, going from nucleotides and adducted nucleotides over nucleoside analogues to oligonucleotides. These nucleic acid constituents play an important role in a variety of biochemical processes. Hence, their isolation, identification, and quantification will undoubtedly help reveal the process of life and disease mechanisms, such as carcinogenesis, and can also be useful for antitumor and antiviral drug research to provide valuable information about mechanism of action, pharmacokinetics, pharmacodynamics, toxicity, therapeutic drug level monitoring, and quality control related to this substance class. Fundamental investigations into their structure, the search for modifications, the occurrence and biochemical impact of structural variation amongst others, are therefore of great value. In view of the related bioanalytical procedures, the coupling of CE to MS has emerged as a powerful tool for the analysis of the complex mixtures of nucleic acid constituents: CE confers rapid analysis and efficient resolution, while MS provides high selectivity and sensitivity with structural characterization of minute amounts of compound. After an introduction about the biochemical and analytical perspectives on the nucleic acid constituents, the different modes of CE used in this field of research as well as the relevant CE-MS interfaces and the difficulties associated with quantitative CE-MS are briefly discussed. A large section is finally devoted to field-oriented applications.

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“…Therefore, molecules with a wide range of molecular weights, solubilities, and polarities, especially nonvolatile, thermally labile molecules, can be analyzed directly without derivatization. With such HPLC/ ESI/MS technology, DNA adducts may be analyzed as substituted bases (58,59), nucleosides (58, 60 -62), nucleotides (63,64), or oligonucleotides (65)(66)(67). In this report, we have demonstrated the application of LC/ MS/SIM and LC/MS/MS/SIM with ESI for the detection and quantitative analysis of the DNA adduct GEG formed in rat and fish liver tissue samples after in vivo exposure to 1,2-dihaloethanes.…”

Section: Application Of Lc/ms/ms Methods For Detection and Quantificatmentioning

confidence: 99%

Analysis of DNA Adduct,S-[2-(N7-Guanyl)ethyl]glutathione, by Liquid Chromatography/Mass Spectrometry and Liquid Chromatography/Tandem Mass Spectrometry

Huang

Jemal

David

et al. 1998

Analytical Biochemistry

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Determination of styrene oxide adducts in DNA and DNA components

Cited by 36 publications

References 17 publications

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Analysis of nucleic acid constituents by on‐line capillary electrophoresis‐mass spectrometry

Analysis of DNA Adduct,S-[2-(N7-Guanyl)ethyl]glutathione, by Liquid Chromatography/Mass Spectrometry and Liquid Chromatography/Tandem Mass Spectrometry

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