Determination of statins by gas chromatography – EI/MRM – Tandem mass spectrometry: Fermentation of pine samples with Pleurotus ostreatus

Sirén, Heli M. M.; Kaijanen, Laura; Kaartinen, Sini; Väre, Monna; Riikonen, Päivi; Jernström, Eeva

doi:10.1016/j.jpba.2014.01.018

Cited by 13 publications

(4 citation statements)

References 24 publications

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“…The amount of total phenolic compounds varied widely in different plant materials (92 phenolic extracts from acetone and methanol were examined) and ranged from 0.2 to 155.3 mg GAE/g dry material [68]. The yields of phenolic compounds were generally variable depending on the tree species, part of the tree used for extraction, as well as the age or place of origin of the tree [69,70]. According to the literature, in forest pine, the total amount of polyphenols ranges between 76 mg GAE /g in dry bark, 17.5 mg GAE/g in needles, and 1.1 mg GAE/g in cork wood [48].…”

Section: Resultsmentioning

confidence: 99%

Investigation of Total Phenolic Content and Antioxidant Activities of Spruce Bark Extracts Isolated by Deep Eutectic Solvents

et al. 2020

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Extracts from spruce bark obtained using different deep eutectic solvents were screened for their total phenolic content (TPC) and antioxidant activities. Water containing choline chloride-based deep eutectic solvents (DESs) with lactic acid and 1,3-propanediol, 1,3-butanediol, 1,4-butanediol, and 1,5-pentanediol, with different molar ratios, were used as extractants. Basic characteristics of the DESs (density, viscosity, conductivity, and refractive index) were determined. All the DESs used behave as Newtonian liquids. The extractions were performed for 2 h at 60 °C under continuous stirring. TPC was determined spectrophotometrically, using the Folin-Ciocalteu reagent, and expressed as gallic acid equivalent (GAE). The antioxidant activity was determined spectrophotometrically by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The TPC varied from 233.6 to 596.2 mg GAE/100 g dry bark; radical scavenging activity (RSA) ranged between 81.4% and 95%. This study demonstrated that deep eutectic solvents are suitable solvents for extracting phenolic compounds from spruce bark.

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Section: Resultsmentioning

confidence: 99%

Investigation of Total Phenolic Content and Antioxidant Activities of Spruce Bark Extracts Isolated by Deep Eutectic Solvents

et al. 2020

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“…There is a great variability in the yields of secondary phenolic metabolites between tree species, between parts of the trees, and even between individuals of different ages and botanical region (provenance) (Kähkönen et al 1999;Harju et al 2003;Willför et al 2003a, b, c;Venäläinen et al 2004;Hovelstad et al 2006;Välimaa et al 2007;Karppanen et al 2008;Sirén et al 2014). The total phenolic concentrations of 76.0, 17.5, and 1.1 mg/g in gallic acid equivalents for dried bark, needles and heartwood, respectively (Kähkönen et al 1999), and 6.7-13.6 mg/g in tannic acid equivalents for wood (Venäläinen et al 2004) of Scots pine (P. sylvestris) have been reported.…”

Section: Metabolites In Parts Of Treesmentioning

confidence: 99%

Bioactive phenolic compounds, metabolism and properties: a review on valuable chemical compounds in Scots pine and Norway spruce

2019

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Phenolics and extracted phenolic compounds of Scots pine (Pinus sylvestris) and Norway spruce (Picea abies) show antibacterial activity against several bacteria. The majority of phenolic compounds are stilbenes, flavonoids, proanthocyanidins, phenolic acids, and lignans that are biosynthesized in the wood through the phenylpropanoid pathway. In Scots pine (P. sylvestris), the most abundant phenolic and antibacterial compounds are pinosylvin-type stilbenes and flavonol-and dihydroflavonol-type flavonoids, such as kaempferol, quercetin, and taxifolin and their derivatives. In Norway spruce (P. abies) on the other hand, the main stilbene is resveratrol and the major flavonoids are quercetin and myricetin. In general, when the results from the literature regarding the activities of flavonoid glycosides and their aglycones against a total of twenty-one microorganisms are summarized, it was found that phenolic glycosides are less active than the corresponding aglycones, although a number of exceptions are also known. The aglycones in plants respond to various kinds of biotic stress. Synergistic effects between aglycones and their glycosides have been observed. Minimum inhibition concentrations of below 10 mg L-1 against bacteria have been reported for gallic acid, apigenin, and several methylated and acylated flavonols present in these industrially important trees. In general, the phenolic compounds are more active against Gram-positive bacteria, but apigenin is reported to exhibit strong activity against Gram-negative bacteria. The present review lists some of the biosynthesis pathways for the antibacterial phenolic metabolites found in Scots pine (P. sylvestris) and Norway spruce (P. abies). The antimicrobial activity of the compounds is collected and compared to gather information about the most effective secondary metabolites.

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Section: Introductionmentioning

confidence: 99%

“…Several analytical methods report the quantitative determination of lovastatin in different matrices. Different approaches and sample preparation protocols are used; the assays are based on HPLC, gas chromatography, electrochemical analysis, and MS. [22][23][24][25][26][27][28][29][30][31][32][33][34] Mass spectrometry is a remarkably sensitive and specific analytical instrumentation that can both determine the structure and the amount of analytes within a sample. [35][36][37] In particular, electrospray ionization (ESI) MS is 1 of the most versatile ion sources used in a wide range of analytical applications; moreover, the MRM analysis mode is a targeted mass spectrometry approach that has being used for decades for accurate quantification of small molecules in complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Assay of lovastatin containing dietary supplement by LC‐MS/MS under MRM condition

et al. 2018

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Monacolin K, the active ingredient present in dietary supplement, is a nutraceutical whose health benefits have been widely documented. A fast approach for the assay of lovastatin in both forms, lactone and acid, by mass spectrometry is presented. The quantitative assay is carried out by high‐performance liquid chromatography‐tandem mass spectrometry by using the multiple reaction monitoring mode and simvastatin and pravastatin as internal standards. The accuracy values ranged from 97 to 101%; the analytical parameter values of limit of quantitation, limit of detection, recovery, and reproducibility were calculated by analyzing fortified samples, confirming the reliability of the proposed approach.

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Determination of statins by gas chromatography – EI/MRM – Tandem mass spectrometry: Fermentation of pine samples with Pleurotus ostreatus

Cited by 13 publications

References 24 publications

Investigation of Total Phenolic Content and Antioxidant Activities of Spruce Bark Extracts Isolated by Deep Eutectic Solvents

Investigation of Total Phenolic Content and Antioxidant Activities of Spruce Bark Extracts Isolated by Deep Eutectic Solvents

Bioactive phenolic compounds, metabolism and properties: a review on valuable chemical compounds in Scots pine and Norway spruce

Assay of lovastatin containing dietary supplement by LC‐MS/MS under MRM condition

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