Assay of lovastatin containing dietary supplement by LC‐MS/MS under MRM condition

Donna, Leonardo Di; Bartella, Lucia; Napoli, Anna; Sindona, Giovanni

doi:10.1002/jms.4202

Cited by 11 publications

(7 citation statements)

References 37 publications

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“…As listed in Tables 1 and 2 and shown in Figure 1, the peaks for lovastatin acid, lovastatin, simvastatin acid, simvastatin and mevastatin are close to each other and have similar UV–Vis spectra. The identities of the potential adulterants were confirmed as lovastatin and lovastatin acid (Figure 4) by comparison of the calculated precursor‐product ion ratios with the ion ratio of the standard, thus allowing accurate determination via LC/ESI‐MS/MS 19,25 . As listed in Table 5, 13 dietary supplements (5 tablets, 7 hard capsules, and 1 soft capsule) contained lovastatin acid and lovastatin at concentrations ranging from <LOQ to 4.12 mg/g and <LOQ to 9.65 mg/g, respectively.…”

Section: Resultsmentioning

confidence: 93%

“…Guo et al reported an LC/ion trap time‐of‐flight mass spectrometry method for the screening of various drug compounds including five statins and two fibrates in dietary supplements 15 . In addition, Cohen et al reported a method for the determination of lovastatin in dietary supplements using an UPLC system equipped with a diode‐array detector and a quadrupole time‐of‐flight mass spectrometer 16 Recently, Di Donna et al analyzed lovastatin‐containing dietary supplements using LC/tandem mass spectrometry (LC/MS/MS) 19 . In these earlier studies, the statins were analyzed in several groups to which they were assigned by their respective optimal detection wavelength because of the similar spectra of the target compounds at the absorption wavelengths in the UV and visible regions.…”

Section: Introductionmentioning

confidence: 99%

“…14 Guo LC/tandem mass spectrometry (LC/MS/MS). 19 In these earlier studies, the statins were analyzed in several groups to which they were assigned by their respective optimal detection wavelength because of the similar spectra of the target compounds at the absorption wavelengths in the UV and visible regions. dissolved in the solvent.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simultaneous screening of dietary supplements for 25 anti‐hyperlipidemic substances using ultra‐performance liquid chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry

Kim

Lim

Choi

et al. 2020

Rapid Comm Mass Spectrometry

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RationaleRecently, illegal dietary supplements containing unauthorized compounds have been seized and internationally publicized with a warning to avoid their consumption. This adulteration can be a serious threat to public health because of insufficient and reliable safety data as well as their undesirable side effects. It is, therefore, essential to rapidly and accurately develop and simultaneously validate analytical methods for these unauthorized anti‐hyperlipidemic substances.MethodsDietary supplements were screened simultaneously for 25 anti‐hyperlipidemic drugs using an ultra‐high‐performance liquid chromatography (UPLC) system with a photodiode array (PDA) detector and LC/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). The method validation, according to ICH guidelines, considered specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, matrix effects, and stability for solid and liquid blank samples. The established UPLC‐PDA and LC/ESI‐MS/MS methods were applied to screen 103 dietary supplements for 25 anti‐hyperlipidemic substances.ResultsUsing the validated methods, the screened samples were found to contain peaks with similar retention times and PDA spectra. By comparing the calculated precursor‐product ion ratios with those of standards, lovastatin and lovastatin acid were detected at concentrations from LOQ to 4.12 mg/g and LOQ to 9.65 mg/g, respectively.ConclusionsThe developed UPLC‐PDA and LC/ESI‐MS/MS analytical methods were applied to 103 real samples, of which 13 samples were found to contain lovastatin and lovastatin acid. In view of the increasing demand for dietary supplements in the treatment of hyperlipidemic diseases, widespread use of these methods will contribute to consumer health by ensuring the safety of dietary supplements.

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Section: Resultsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous screening of dietary supplements for 25 anti‐hyperlipidemic substances using ultra‐performance liquid chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry

Kim

Lim

Choi

et al. 2020

Rapid Comm Mass Spectrometry

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show abstract

“…It offers good detection of monacolin analogs in RYR. Donna et al [68] uses the multiple reaction monitoring (MRM) mode and HPLC-MS/MS as the analytical method: simvastatin and pravastatin were used as indicators to determine the acid and lactone monacolin K content in RYR. Different detectors have different detection ranges.…”

Section: The Detection Methods Of Monacolin K Of Ryrmentioning

confidence: 99%

Quality and Authenticity Control of Functional Red Yeast Rice—A Review

Song

Luo

et al. 2019

Molecules

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Red yeast rice (RYR) is made by fermenting the rice with Monascus. It is commonly used in food colorants, dyeing, and wine making in China and its neighboring countries. Nowadays RYR has two forms on the market: common RYR is used for food products, the other form is functional RYR for medicine. However, some researchers reported that commercial lovastatin (structure is consistent with monacolin K) is illegally added to common RYR to meet drug quality standards, so as to imitate functional RYR and sell the imitation at a higher price. Based on current detection methods, it is impossible to accurately distinguish whether functional RYR is adulterated. Therefore, it is especially important to find a way to authenticate functional RYR. In the current review, the advances in history, applications, components (especially monacolins, monacolins detection methods), quality standards, authentication methods and perspectives for the future study of RYR are systematically reviewed.

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“…Conventionally, they are analyzed by gas chromatography (GC) or high‐performance liquid chromatography (HPLC) coupled with mass spectrometry (MS), ultraviolet, and electrochemical detection 17–23 . MS is certainly the most appropriate tool for a specific and sensitive analysis 24,25 ; moreover, this technique allows the use of labeled internal standards, which assure a high grade of accuracy in the analysis 26,27 . In this work, we developed a new approach based on the use of paper spray (PS) ionization coupled with tandem MS. PS is an ambient ion source, which allows to create ions directly in their native state, performing fast analysis of a wide variety of compounds present in complex mixtures, with a minimal sample pretreatment and without any chromatographic separation.…”

Section: Introductionmentioning

confidence: 99%

Paper spray tandem mass spectrometry: A rapid approach for the assay of parabens in cosmetics and drugs

et al. 2020

Self Cite

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A fast methodology for the assay of parabens in drug and cosmetic preparations has been presented. The procedure developed is based on paper spray tandem mass spectrometry and isotope dilution approach. For each investigated paraben, the corresponding labeled standard has been used in order to improve the accuracy and reproducibility of the analyses. The MS experiments have been performed under MRM conditions, monitoring the transitions [M‐H]− → m/z 92 and [M‐H]− → m/z 98, respectively, for each analyte and the corresponding labeled internal standard. The quantitative assay has been performed using a calibration curve built from 2 to 15 mg/L. The method accuracy, in all case near 100%, was evaluated using fortified samples at two concentration levels, which are representative of the lower and the higher portion of calibration curve. The good values of LOQ, LOD, and reproducibility confirm the consistency of the developed approach.

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Assay of lovastatin containing dietary supplement by LC‐MS/MS under MRM condition

Cited by 11 publications

References 37 publications

Simultaneous screening of dietary supplements for 25 anti‐hyperlipidemic substances using ultra‐performance liquid chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry

Simultaneous screening of dietary supplements for 25 anti‐hyperlipidemic substances using ultra‐performance liquid chromatography and liquid chromatography/electrospray ionization tandem mass spectrometry

Quality and Authenticity Control of Functional Red Yeast Rice—A Review

Paper spray tandem mass spectrometry: A rapid approach for the assay of parabens in cosmetics and drugs

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