Determination of sertraline and N‐desmethylsertraline in human plasma by CE with LIF detection

Abstract

A method has been developed for the analysis of the antidepressant drug sertraline together with its main metabolite N-desmethylsertraline (DMS) in human plasma. It is based on CE with LIF detection (lambda = 488 nm). A SPE procedure is employed for biological sample pretreatment, followed by a derivatization step with FITC; reboxetine was the internal standard. The effect of CD, acetone and N-methyl-D-glucamine (GLC) as constituents of the BGE for analyte separation was investigated. The final BGE consisted of 20 mM carbonate buffer, pH 9.0, with 2.5 mM heptakis(2,6-di-O-methyl)-beta-CD, 50 mM GLC and 20% v/v acetone. With 30 kV applied voltage, the electrophoretic run is completed in 7.5 min. Linearity was observed in the plasma concentration range from 3.0 to 500 ng/mL for sertraline and 4.0 to 500 ng/mL for DMS. Extraction yield was >97.1%, precision - expressed as RSD% - was <3.7, accuracy (recovery) was >95.6%. Due to its sensitivity and selectivity, the method was suited for the analysis of plasma samples from patients undergoing therapy with sertraline.

Quality control of herbal medicines by capillary electrophoresis: Potential, requirements and applications

“…determination of active constituents or impurities, chiral separations and the analysis of pharmaceutical excipients, CE has established its position as reliable and sensitive analytical tool within the last years already [3]. For example, some of the most recent applications in this respect are the determination of penicillamine in pharmaceuticals and human plasma [4], the analysis of sertraline and its derivatives [5], or the separation of barbiturates by CE [6]. Yet, analysis of medicinal plants is a different, in some respect even more challenging task [7,8].…”

Electrodriven methods for the enantioseparation of second‐generation antidepressant drugs: An update

“…The labelling reaction drastically modifies the analyte properties and the enantioseparation may become more problematic. To the best of our knowledge, only one method has been published until now, which uses CE-LIF for the quantitative analysis of an antidepressant (sertraline) in biological fluids [72], and it is not enantioselective. Our research group is currently investigating a CE-LIF method for the determination of the enantiomers of fluoxetine and its main active metabolite norfluoxetine after derivatisation with FITC, using heptakis-2,6-di-O-methyl-b-CD as the chiral selector (Musenga, A. et al, manuscript in preparation).…”

Determination of vigabatrin in human plasma by means of CE with LIF detection

“…We previously demonstrated [28] that the main effect of GLC is from the reduction of the EOF mobility. This is confirmed in the present study: the relative differences of the apparent mobilities increase with increasing GLC concentration, thus resulting in improved resolution ( Table 1).…”

“…Following our previous experience [28], we applied GLC. This compound acts mainly by means of its positive charge (its pK a value is 9.2).…”