Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism

Lahnsteiner, Franz; Berger, Beate; Weismann, T.; Patzner, Robert

doi:10.1016/s0044-8486(98)00243-9

Cited by 327 publications

(224 citation statements)

References 34 publications

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“…Surprisingly, the variation of sired offspring was very high with gold:green ratios ranging from 1:99 to 98:2, but these proportions were not significantly influenced by the large variation in velocity (from 112 to 163 mm·s 21 ) of competitive spermatozoa. However, published results do not always show a positive effect of velocity; for example, the highest sperm velocities were associated with a reduction of fertilization rate in rainbow trout (Lahnsteiner et al 1998).…”

Section: Discussionmentioning

confidence: 87%

“…When the effect of sperm number is suppressed, then, according to published results in fishes with external in vitro fertilization, fertilization success reflects sperm motility (Lahnsteiner et al 1998) and velocity of spermatozoa (Gage et al 2002(Gage et al , 2004. Surprisingly, the variation of sired offspring was very high with gold:green ratios ranging from 1:99 to 98:2, but these proportions were not significantly influenced by the large variation in velocity (from 112 to 163 mm·s 21 ) of competitive spermatozoa.…”

Section: Discussionmentioning

confidence: 96%

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Spermatozoal competition in common carp (Cyprinus carpio): what is the primary determinant of competition success?

Linhart¹,

Rodina²,

Gela³

et al. 2005

Reproduction

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The percentage of sperm motility (92-100%), spermatozoan velocity (112 -163 mm·s 21 ) and control hatching rates (83-96%) were evaluated for each of six gold and five green male common carp (Cyprinus carpio). In all 30 possible paired combinations of sperm-competition tests, hatching rates of 90 -97% were achieved. The mean percentage of offspring sired was strongly influenced by the male used (P < 0.001, R 2 5 0.91). The best male sired an average of 88% of the offspring in its competition tests, and the worst male sired only 5%. Spermatozoan-quality parameters could explain only part of the variation in male competitive ability. The male effects alone explained 91.4% of the observed variance, consisting of 17.1% explained by spermatozoan motility and 32.5% by control hatching rates in single fertilizations. Undetermined male effects explained 41.8%. The velocity of spermatozoa had no effect on the outcome of sperm competition. Neither was there any link between spermatozoan velocity and hatching rate in a control hatching test, whereas there was an effect of motility on hatching rate in this same test.Reproduction ( IntroductionSperm competition occurs when the spermatozoa of several males compete for fertilization. Competition is a major force for spermatozoan selection, but its implications for mating systems and on life-history evolution are only just beginning to be understood (Parker 1998, Gage et al. 2004. Trade-offs between gamete characteristics and relative gametic investment has been modelled by evolutionary stable strategy (ESS) approaches, when the optimal strategy for a male depends on the strategy adopted by other individuals in the population , Parker 1998, Petersen & Warner 1998. All of the models assume that males obtain more fertilization success by releasing more sperm, this being done at a cost to investment in other fitness-enhancing functions such as growth, finding additional males, investment in female function, mate defence, territoriality or releasing more sperm in other spawnings. As applied to fish, sperm-competition trials with paternity analysis have been conducted in several salmonid species (Gage et al. 1995, Gile & Ferguson 1995, Liley et al. 2002 and recently also in cyprinids (Kortet et al. 2004, Reichard et al. 2004a, 2004b or other species such as bluegill sunfish (Lepomis macrochirus; Burness et al. 2004). In rainbow trout (Oncorhynchus mykiss) male potency, defined as the ability of males to sire progeny when their sperm is pooled with that of other males, was not affected by the female gametes used, and decreased significantly when sperm concentrations were equalized (Gile & Ferguson 1995). Sperm vary in morphology, behaviour (speed, trajectory, percentage of sperm motility, etc.) and longevity in fishes. Currently, the mechanism of this variation is poorly explained (Cosson et al. 1999). There appear to be intraspecific differences that may correlate with male-mating type in Atlantic salmon (Salmo salar; Gage et al. 1995). Fishes show one of the widest ranges ...

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 96%

Spermatozoal competition in common carp (Cyprinus carpio): what is the primary determinant of competition success?

Linhart¹,

Rodina²,

Gela³

et al. 2005

Reproduction

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show abstract

“…This mechanism would be similar to the peristaltic movements of the uterine tube in mammals. By contrast, the sperm duct epithelium is related to seminal f luid production (Kobayashi et al, 2012;Spadella et al, 2012), and according to Lahnsteiner et al (1998), feeds the spermatozoa and keeps them motionless.…”

Section: Discussionmentioning

confidence: 99%

Morphology of the urogenital papilla and its component ducts in Astyanax altiparanae Garutti & Britski, 2000 (Characiformes: Characidae)

et al. 2015

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The histological description of the urogenital papilla is an important tool to comprehension of the reproductive mechanisms in fish, as well as a pre-requisite to germ cell transplantation in adult fish, besides to be a good biological indicator to environmental changes. Was performed the histological description of the urogenital papilla and its component ducts in the tetra Astyanax altiparanae. The genital and urinay ducts pass separately throughout most part of its extension, joining in a single duct before opening. In males this opening is asymmetric and seems to have double origin, being completely surrounded by striated muscle fibers, while in females it is symmetric and the muscle fibers does not surround it totally. Spermatic duct and oviduct undergo changes throughout their extension, mainly in the morphology of the surrounding epithelium. In the spermatic duct, squamous epithelial cells change to columnar and cuboid with possible secretory activity, close to testes. In the oviduct, anteriorly epithelial cells are also squamous, however, close to ovary there are lamellae composed by a pseudostratified epithelium with columnar and cuboid cells. The urinary duct is highly similar for both sexes presenting globoid cells, which description is known in mammals, however, rare in fish.A descrição histológica da papila urogenital é uma importante ferramenta para a compreensão dos mecanismos reprodutivos em peixes, assim como um pré-requisito para a realização do transplante de células germinativas em peixes adultos, além de um bom indicador biológico de possíveis alterações ambientais. Foi realizada a investigação histológica da papila urogenital e seus ductos constituintes no lambari Astyanax altiparanae. Os ductos genital e urinário ocorrem separadamente ao longo de maior parte de sua extensão, entretanto, unem-se em um ducto simples antes de abrir para o meio externo. Nos machos esta abertura é assimétrica e parece ter dupla origem, sendo completamente envolvida por fibras musculares estriadas, enquanto nas fêmeas ela é simétrica e as fibras musculares não a envolve totalmente. O ducto espermático e oviducto sofrem alterações ao longo de sua extensão, principalmente na morfologia do epitélio que os envolve. No ducto espermático as células epiteliais passam de pavimentosas a colunares e cuboides, com possível atividade secretora, à medida que se aproxima dos testículos. No oviducto, anteriormente as células também são epiteliais pavimentosas, entretanto, próximo aos ovários, formam-se lamelas compostas por um epitélio pseudoestratificado composto por células cuboides e colunares. O ducto urinário é bastante similar em ambos os sexos apresentando células globosas, cuja descrição é conhecida em mamíferos, porém rara em peixes.

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“…Sperm motility was stimulated with the following buffers: AS1: 40 mM NaHCO3, 20 mM Tris, 0.5% albumin, pH 8.5; 101 mOsmkg -1 (Kowalski et al, 2010); AS2: 100 mM NaCl, 10 mM Tris, 0.5% albumin, pH 9.0; 199 mOsmkg -1 (Lahnsteiner et al, 1998); AS3: 0.4% NaCl, 0.3% urea, 0.5% albumin, pH 7.7; 181 mOsmkg -1 (Babiak et al, 1997a); AS4: 0.45 g NaCl, 0.1211 g Tris, 0.1126 g glycine, 0.0056 g CaCl2, 0.5% albumin, (50 mL), pH 9.0; 344 mOsmkg -1 (Billard et al, 1995); AS5: 120 mM NaCl, 0.5% albumin, pH 7.2; 225 mOsmkg -1 (Babiak et al, 1997b;Cejko et al, 2010b);…”

Section: Optimum Buffer Selectionmentioning

confidence: 99%

“…For example, the LIN parameter of sperm in pikeperch (Sander lucioperca L.) was affected by salt solutions used as an activating solution (Sarosiek et al, 2004). The results helped to select the optimum activating liquid for orfe sperm (AS2) (Lahnsteiner et al, 1998). Moreover, further detailed research is needed into the role of ionic composition of activating liquids in the mechanism of the activation of orfe spermatozoa motility.…”

Section: Optimum Buffer Selectingmentioning

confidence: 99%

Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

Sarosiek

Cejko

Targońska

et al. 2012

Italian Journal of Animal Science

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In this study the effect of six activating buffers on the movement parameters of sperm were determined and short-term storage of semen in TLP buffer was attempted (0.292 g NaCl; 0.012g KCl; 0.011g CaCl2; 0.004g MgCl2; 0.105g NaHCO3; 0.002g NaH2PO4; 50 mL; pH 8.6). Sperm was collected from five orfe individual, and spermiation was stimulated by means of an intraperitoneal Ovopel injection. The basic parameters of spermatozoa motility were measured after the semen was diluted with six different activating solutions, previously used successfully in other fish species. The motility analysis was conducted on a Crismas apparatus. Additionally, short-term storage of semen in TLP buffer was attempted. Subsequently, motility parameters were verified after 0 (Control), 24 and 120 h of storage at 4°C. It has been found that Lahnsteiner's buffer (100 mM NaCl, 10 mM Tris, 0.5% albumin, 199 mOsmkg -1 ) was found to be the most effective in sperm activation. In this paper, the spermatozoa motility of colourful ide is indicated for the first time. Finally, there was a successful attempt at short-term sperm storage for five days. For artificial insemination, it is very important to select the most effective solution to stimulate sperm motility. Data regarding sperm manipulation of orfe are scarce, so the aim of the study was to determine the basic sperm quality parameters of the colour ide form, i.e. Leuciscus idus aberr orfus.

show abstract

Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism

Cited by 327 publications

References 34 publications

Spermatozoal competition in common carp (Cyprinus carpio): what is the primary determinant of competition success?

Spermatozoal competition in common carp (Cyprinus carpio): what is the primary determinant of competition success?

Morphology of the urogenital papilla and its component ducts in Astyanax altiparanae Garutti & Britski, 2000 (Characiformes: Characidae)

Spermatozoa motility and short-term sperm storage of colourful orfe (Leuciscus idus aberr orfus)

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