Determination of salbutamol enantiomers in human urine using heptakis(2,3‐di‐O‐acetyl‐6‐O‐sulfo)‐β‐cyclodextrin in nonaqueous capillary electrophoresis

Servais, Anne-Catherine; Chiap, Patrice; Hubert, Philippe; Crommen, Jacques; Fillet, Marianne

doi:10.1002/elps.200405854

Cited by 27 publications

(25 citation statements)

References 32 publications

(23 reference statements)

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“…Various chiral selectors have been used in enantioselective NACE, such as various kinds of CDs [3][4][5][6], low-molecularmass chiral counterions [24][25][26][27], chiral crown ether [28], and so on. But SC has not been utilized in enantioselective NACE yet.…”

Section: Resultsmentioning

confidence: 99%

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Lin

LiangJun

et al. 2007

Electrophoresis

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The enantiomeric separation of some poorly water-soluble organophosphorus pesticides (OPs) has been investigated using nonaqueous solvent and aqueous-organic solvent systems. In this work, sodium cholate (SC) either with SDS or gamma-CD was used to achieve enantiomeric separations of four neutral and poorly water-soluble OPs, i.e., profenofos, prothiofos, sulprofos, and pyraclofos. Electrophoretic medium consisted of a mixture of methanol (MeOH) with ACN (4:1 v/v) or a mixture of MeOH with H(2)O and ACN (5:4:1 v/v/v). On one hand, NACE was applied for enantiomeric separation of pyraclofos using a large amount of chiral and achiral surfactants (SC and SDS). On the other hand, H(2)O was added to act as a solvent additive to increase the solubility of gamma-CD in the organic solvents such as MeOH and ACN, in which the solubility of gamma-CD was very low. The presence of H(2)O was found to be particularly useful for the enantiomeric separation of profenofos, prothiofos, and sulprofos. In this way, the range of application of the neutral CDs in CE has been extended. In addition, SC was used as the only electrolyte. The proposed method has been applied for the analysis of soil samples.

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Section: Resultsmentioning

confidence: 99%

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Lin

LiangJun

et al. 2007

Electrophoresis

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“…It cooperated also in bioanalytical applications separating enantiomers of salbutamol with heptakis-(2,3-di-O-acetyl-6-O-sulfo)-b-CD in urine samples under nonaqueous EKC conditions [65,82] (for details see Table 1). …”

Section: Ion-pairing Reagentsmentioning

confidence: 99%

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

Mikuš

Maráková

2009

Electrophoresis

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An analysis of recent trends indicates that CE can show real advantages over chromatographic methods in ultratrace enantioselective determination of biologically active compounds in complex biological matrices. It is due to high separation efficiency and many applicable in-capillary electromigration effects in CE (countercurrent migration, stacking effects) enhancing significantly (enantio)separability and enabling effective sample preparation (preconcentration, purification, analyte derivatization). Other possible on-line combinations of CE, such as column coupled CE-CE techniques and implementation of nonelectrophoretic techniques (extraction, membrane filtration, flow injection) into CE, offer additional approaches for highly effective sample preparation and separation. CE matured to a highly flexible and compatible technique enabling its hyphenation with powerful detection systems allowing extremely sensitive detection (e.g. LIF) and/or structural characterization of analytes (e.g. MS). Within the last decade, more as well as less conventional analytical on-line approaches have been effectively utilized in this field and their practical potentialities are demonstrated on many new application examples in this article. Here, three basic areas of (enantioselective) drug bioanalysis are highlighted and supported by a brief theoretical description of each individual approach in a compact review structure (to create integrated view on the topic), including (i) progressive enantioseparation approaches and new enantioselective agents, (ii) in-capillary sample preparation (preconcentration, purification, derivatization), and (iii) detection possibilities related to enhanced sensitivity and structural characterization.

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“…Servais et al [168] described a validated NACE method for the determination of salbutamol enantiomers in urine samples. The single-isomer, negatively charged HDAS-b-CD was used as chiral selector and SPE was used for sample cleanup prior to the CE separation.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Chiral separations by capillary electrophoresis: Recent developments and applications

2006

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Determination of salbutamol enantiomers in human urine using heptakis(2,3‐di‐O‐acetyl‐6‐O‐sulfo)‐β‐cyclodextrin in nonaqueous capillary electrophoresis

Cited by 27 publications

References 32 publications

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

Chiral separations by capillary electrophoresis: Recent developments and applications

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