Determination of S-adenosylmethionine and S-adenosylhomocysteine in blood plasma by UPLC with fluorescence detection

Ivanov, Alexander; Dubchenko, Ekaterina; Kruglova, Maria Petrovna; Virus, Edward Danielevich; Булгакова, Полина Олеговна; Александрин, В. В.; Федосеев, А. Н.; Boyko, A. N.; Grachev, Sergej Vitalievich; Кубатиев, Аслан Амирханович

doi:10.1016/j.jchromb.2019.06.032

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…Due to the close metabolic relationship, plasma SAM and SAH levels show a fairly high correlation in normal conditions [9,26,27]. However, since our study showed that COVID-19 patients with severe lung damage showed an increase in plasma SAM levels, but no significant increase in SAH levels, this clearly indicates a dysregulation of transmethylation in COVID-19.…”

Section: Discussionmentioning

confidence: 49%

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Plasma S-Adenosylmethionine is Associated with Lung Injury in COVID-19

Kryukov¹,

Ivanov

Karpov³

et al. 2021

Preprint

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Objective: S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are indicators of global transmethylation and may play an important role as markers of severity of COVID-19. Methods: The levels of plasma SAM and SAH were determined in patients admitted with COVID-19 (n = 56, mean age = 61). Lung injury was identified by computed tomography (CT) in accordance with the CT0-4 classification. Results: SAM was found to be a potential marker of lung damage risk in COVID-19 patients (SAM > 80 nM; CT3,4 vs. CT 0-2: relative ratio (RR) was 3.0; p = 0.0029). SAM/SAH > 6.0 was also found to be a marker of lung injury (CT2-4 vs. CT0,1: RR = 3.47, p = 0.0004). Interleukin-6 (IL-6) levels were associated with SAM (r= 0.44, p = 0.01) and SAH (r= 0.534, p = 0.001) levels. Conclusions: High SAM levels and high methylation index are associated with the risk of lung injury in COVID-19 patients. The association of SAM and SAH with IL-6 indicates an important role of transmethylation in the development of cytokine imbalance in COVID-19 cases.

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Section: Discussionmentioning

confidence: 49%

“…GSH and Hcy levels were determined by liquid chromatography as described in a previous study [25]. SAM and SAH levels were determined by liquid chromatography -fluorescence detection as described in a previous study, with some modifications [26]. A UPLC ACQUITY system (Waters, Milford, MA, USA) was used in both these cases.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Plasma S-Adenosylmethionine is Associated with Lung Injury in COVID-19

Kryukov¹,

Ivanov

Karpov³

et al. 2021

Preprint

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“…To our knowledge, there are no data available about MTA levels in urine and no reference values are known. Earlier it was shown that the ratio of MTA/SAM in plasma was about 0.15 , which is much higher than in urine (∼0.04). This may be because of MTA absorption by the tubules of the kidneys (however, there are no data on the clearance of the MTA) or because of peculiarities of the chemical derivatization of the method during which a portion of SAM may be spontaneously converted into SAH.…”

Section: Resultsmentioning

confidence: 88%

Determination of S‐adenosylmethionine, S‐adenosylhomocysteine, and methylthioadenosine in urine using solvent‐modified micellar electrokinetic chromatography

et al. 2019

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A new approach for direct determination of S-adenosylmethionine (SAM), Sadenosylhomocysteine (SAH), and methylthioadenosine (MTA) in urine was developed based on MEKC by using SDS modified with isobutanol in the presence of PEG-300. Analytes were first extracted with grafted phenylborononic acid. Using a 50 µm internal diameter silica capillary of 32 cm total length filled with 0.05 M SDS, 0.05 M H 3 PO 4 , 5% (v/v) isobutanol, and 10% (v/v) PEG-300, LOQ of 0.15 µM for SAM and SAH, and 0.2 µM for MTA was reached. Accuracy was 92% for MTA, 109% for SAH, and 105% for SAM, intra-and interday imprecision were <2.5 and ≤3%, respectively. The total time of analysis for one sample was 10 min. Analysis of 30 urine samples from healthy volunteers showed that the median SAM and SAH levels were 12.1 and 0.73 µM, respectively. MTA levels, which were determined in urine for the first time (according to our data), were 0.43 µM, and these values correlated well with the SAM level (r = 0.748, p < 0.01).

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“…SAM is also involved in detoxification metabolism mechanisms through methylation of catechol compounds and other substances in the liver. Moreover, a recent study suggested that SAM levels contribute to extended life span in Drosophila [28][29][30][31][32][33].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic analysis of SMP30/GNL knockout mice treated with fermented vegetable and fruit extract (OM-X®)

Wakame

Takahata²,

Miyake³

et al. 2020

FFHD

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Background: A dietary supplement developed in Japan, OM-X®, is the result of extended fermentation of dozens of edible vegetables, fruits, seaweeds, mushrooms, and includes 12 strains of Lactic acid bacteria (LAB) and Bifidobacterium. The supplements powerful antioxidant, anti-inflammatory properties, and regulatory effect on metabolizing liver enzymes have been reported.Senescence marker protein-30 (SMP30)/Gluconolactonase (GNL) Knockout (KO) mice, which lack the ability to biosynthesize Vitamin C (VC) in the body, were used in this study. The mice exhibited decreased antioxidant capacity in the blood, decreased liver function, and had poor hair growth due to the VC deficiency. The aim of the study was to examine the effects of oral administration of OM-X® on VC deficiency from the viewpoint of comprehensive analysis of liver metabolism.Methods: SMP30/GNL KO and C57BL/6 (WT) mice were used in this study. The KO mice were divided into the following three groups: VC non-administration (VC minus), VC (0.15 W/V %) administration (VC plus), and no VC plus 0.6% OM-X® administration (OM-X®) groups (n = 6 per group). Mice were kept under experimental conditions until they were euthanized at week 8 (age: 16 weeks old). Body weight and plasma biochemistry levels were measured.. Metabolomic analyses of harvested livers were performed.Results: In the VC minus group, a significant decrease in body weight and significant changes in plasma biochemistry parameters compared with the WT control or VC plus groups was observed. In the OM-X® group, significant suppression or a suppressive trend was observed compared with the VC minus group. Liver metabolomic analysis showed that total adenylate levels decreased in the VC minus group, whereas OM-X® administration significantly elevated total adenylate levels. In addition, the ratios of Glutathione (GSH)/ Glutathione disulfide (GSSG) and S-adenosylmethionine (SAM)/ S-adenosyl-L-homocysteine (SAH) and total glutathione levels were all significantly higher in the OM-X® group than in the VC minus group. The glycerol 3-phosphate/ Dihydroxyacetone phosphate (DHAP) and putrescine/spermidine ratios were elevated in the VC minus group, whereas it was significantly lower in the OM-X® group.Conclusion: The results showed that in SMP30/GNL KO mice, some organ damage may have occurred during VC deficiency, as indicated by weight loss, hepatic injury, and changes in triglyceride-related markers. OM-X® had an effect on energy charge maintenance by elevating total adenylate levels, inducing antioxidant capacity via enhancing glutathione levels, and promoting protein synthesis, including polyamine synthesis. Altogether, the results revealed that OM-X® prevents the adverse biological changes caused by VC deficiency in SMP30/GNL KO mice.Keywords: OM-X®, SMP30/GNL KO mice, Vitamin C, metabolomic analysis.

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Determination of S-adenosylmethionine and S-adenosylhomocysteine in blood plasma by UPLC with fluorescence detection

Cited by 13 publications

References 31 publications

Plasma S-Adenosylmethionine is Associated with Lung Injury in COVID-19

Plasma S-Adenosylmethionine is Associated with Lung Injury in COVID-19

Determination of S‐adenosylmethionine, S‐adenosylhomocysteine, and methylthioadenosine in urine using solvent‐modified micellar electrokinetic chromatography

Metabolomic analysis of SMP30/GNL knockout mice treated with fermented vegetable and fruit extract (OM-X®)

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