Determination of riboflavin in urine by capillary electrophoresis–blue light emitting diode-induced fluorescence detection combined with a stacking technique

Su, An Kai; Lin, Cheng Huang

doi:10.1016/s1570-0232(02)00849-8

Cited by 64 publications

(31 citation statements)

References 26 publications

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“…LEDs have recently become commercially available and are currently in use in many fields. Recently, they have been introduced as excitation sources for fluorescence detection in MEKC by Lin's research group [44,[208][209][210]. The beam quality (beamwidth, intensity, coherence, and monochromaticity) of an LED is still not at par with that of a laser, so the sensitivity is much lower than with LIF detection.…”

Section: Lif Spectroscopymentioning

confidence: 99%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on-line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in-capillary reactions and coupling with flow-injection systems) and improvements in resolution obtained by changing the composition of the BGE (e.g., with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.

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Section: Lif Spectroscopymentioning

confidence: 99%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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“…Recently, Hillebrand et al [9] reported a LED-FD in CE using a pulsed mode-operating ultraviolet LED. Su and Lin [10] determined riboflavin in urine using a blue LED by CE combined with sample stacking. In addition, Wang and Morris [11] have demonstrated a continuous mode-operating UV LED in an analyte velocity modulation device applied for microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Yang

Tan

Guan

2005

Talanta

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“…As it can be observed in Table 2 the prediction results obtained for the three algorithms in the studied urine samples are in agreement with the values previously mentioned. 13 A recovery study was carried out by spiking the urine samples with the analyte, by duplicate, at one concentration level. The signal of the analyte highly overlapped with the fluorescent matrix constituents (Fig.…”

Section: Urine Samplesmentioning

confidence: 99%

“…When RF intake is low, excretion is proportional to the intake [3]. The most widely used analytical methods for the quantification of RF in human urine samples are liquid chromatography (LC)-fluorescence detection (FD) [8], LC-tandem mass spectrometry (MS/MS) [9,10], fluorescence with flow-injection analysis [11], capillary electrophoresis (CE) [12,13], voltammetry [14] and chemiluminescence [15]. These methods require rigorous extraction steps, the use of significant amounts of organic solvents and long analysis time.…”

Section: Introductionmentioning

confidence: 99%

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

Privitera

Lozano

2017

Microchemical Journal

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A new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation-emission fluorescence matrix (EEFM) data in standard addition mode and second-order chemometric analysis. The method is simple, fast and eco-friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial leastsquares/residual bilinearization (U-PLS/RBL) and multidimensional partial leastsquares/residual bilinearization (N-PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.

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Determination of riboflavin in urine by capillary electrophoresis–blue light emitting diode-induced fluorescence detection combined with a stacking technique

Cited by 64 publications

References 26 publications

MEKC: An update focusing on practical aspects

MEKC: An update focusing on practical aspects

A collinear light-emitting diode-induced fluorescence detector for capillary electrophoresis

Development of a second-order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

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