Determination of residues of enrofloxacin and its metabolite ciprofloxacin in biological materials by capillary electrophoresis

Barrón, D.; Jiménez-Lozano, E; Cano, Jean Paul; Barbosa, J.

doi:10.1016/s0378-4347(01)00214-6

Cited by 85 publications

(56 citation statements)

References 20 publications

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“…The limit of quantification (LOQ) was defined as the sample concentration that produced a peak height of ten times the signal-to-noise ratio [19,23]. , respectively, for the same fluoroquinolones.…”

Section: Validation Resultsmentioning

confidence: 99%

“…Because the milk matrix contains interferences such as proteins, lactose, and inorganic ions, the success of the extraction procedure depended on the effective deprotenization and washing steps. Dichloromethane has been used to extract ciprofloxacin and enrofloxacin from chicken muscle tissue samples with reported recoveries of 74 and 54% for enrofloxacin and ciprofloxacin, respectively [23]. A two-step solid-phase extraction procedure was developed by Lara et al [20] using Oasis MAX and HLB cartridges without protein precipitation.…”

Section: Extraction and Clean-upmentioning

confidence: 99%

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Effective Separation and Simultaneous Determination of Four Fluoroquinolones in Milk by CE with SPE

Sun

Zhao

2008

Chroma

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A simple and sensitive capillary electrophoresis method with solid phase extraction was developed for the determination of sarafloxacin, ciprofloxacin, enrofloxacin and flumequine in milk. Solid-phase extraction with Oasis HLB cartridge column was used for the isolation of four fluoroquinolones in raw milk from a farm and fresh milk sample. Separation conditions of CE, including running buffer, voltage and temperature, were investigated and optimized. Baseline separation was achieved for the four fluoroquinolones under the developed conditions. Correlation coefficients greater than 0.9998 were obtained for all fluoroquinolones with a dynamic range from 1 up to 100 mg L -1. The intra-day precision was less than 5%, and the inter-day precision was less than 6%. The method recoveries of four fluoroquinolones were in the range of 70.9-90.6%. The detection limits for sarafloxacin, ciprofloxacin, enrofloxacin and flumequine was 19.8, 15.2, 13.3 and 15.9 lg kg -1

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Section: Validation Resultsmentioning

confidence: 99%

Section: Extraction and Clean-upmentioning

confidence: 99%

Effective Separation and Simultaneous Determination of Four Fluoroquinolones in Milk by CE with SPE

Sun

Zhao

2008

Chroma

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“…The sensitivity of our method is comparable to ELISA and electrophoresis, and may not have an advantage over routine analytical methods such as HPLC, LC/MS, and ELISA in terms of sensitivity. [12][13][14][15][16][17][18][19][20][21][22][23] However, our approach is advantageous over these methods since it has a short assay time, a simple experimental protocol, and low background signals. We believe that our strategy has wide potential for use as a detection method for small molecules that have corresponding antibodies or capturing agents.…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11] Along with tightening up the regulations on FQ use, various analytical methods have been developed for determining or screening FQ residues, including high-performance liquid chromatography (HPLC), [12][13][14] liquid chromatography-mass spectrometry (LC/MS), 15,16 enzyme-linked immunosorbent assays (ELISA), [17][18][19][20] and capillary electrophoresis. [21][22][23] Although these methods are routinely used with high sensitivity, general use of these methods is sometimes hampered by the need for sophisticated and time-consuming processes. In this respect, a new method with a simple experimental protocol is demanded, while sustaining high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Detection of Enrofloxacin and Its Metabolite Ciprofloxacin Using Gold Nanoparticles and Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Seo

Bae

et al. 2014

ANAL. SCI.

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This paper describes a new, simple, and sensitive method for detecting two fluoroquinolones: enrofloxacin and its metabolite ciprofloxacin, which are widely used as drugs for humans and animals. We utilized gold nanoparticles (AuNPs) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a matrix-free format. An antibody for the drug was immobilized on a chip based on self-assembled monolayers (SAMs) on gold, and AuNPs were decorated with the drug along with a large excess of small molecules, called amplification tags (Am-tags). In this strategy, target drugs in solution bound to the antibody on the chip compete with the AuNP-immobilized drugs. The presence of targets was verified by the amplified LDI-TOF MS signals of Am-tags on AuNPs.

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“…The HPTLC method has been developed for the determination and the purity control of CF in coated tablets [14,15]. Other different methods were reported such as spectrophotometry [16][17][18][19][20][21][22], fluorimetry [22][23][24], capillary electrophoresis [25][26][27][28][29][30][31][32] and immunoassay [33,34]. Both spectrophotometric [35][36][37][38] and chemiluminescent [39,40] detection were proposed as automatic spectroscopy procedures.…”

Section: Introductionmentioning

confidence: 99%

Preconcentration and Determination of Ciprofloxacin with Solid-phase Microextraction and Silica-coated Magnetic Nanoparticles Modified with Salicylic Acid by UV-Vis Spectrophotometry

Neshin¹,

Ebrahimi²

2018

Eurasian J Anal Chem

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In the present age, the pharmaceutical application continues to increase and this results in the environmental pollution. As a result, pharmaceutical control is a common technique in many labs. This project is on the improvement of the procedure to determine a small amount of ciprofloxacin (CF) in bio-environmental sample. Regarding the fact that each method requires preliminary measures to preconcentrate and prepare analyte prior to measurement and recognition. In recent study, preconcentration of samples containing CF (as antibiotic) based on solid-phase microextraction method using silica-coated magnetic nanoparticles modified with salicylic acid (SA) was proposed which acted as sorbent. In addition, UV-Vis spectrophotometry was carried out to determine the amount of CF. The wavelength of maximum absorption (λmax) for this drug was 275 nm. Also, the optimum conditions of various parameters such as pH of solution, amount of magnetic nanoparticles, time, the effect of stirring speed on the extraction and desorption, the rate of centrifugation, the volume of aqueous and organic-phases, temperature and salt effect were investigated. The dynamic range was obtained between 0.05-0.15 mg L -1 , LOD and RSD (%) were 0.002 mg L -1 and 1.878 respectively. Results showed using this procedure increased the concentration factor to 68.36. Relative recovery was achieved by spike method.

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Determination of residues of enrofloxacin and its metabolite ciprofloxacin in biological materials by capillary electrophoresis

Cited by 85 publications

References 20 publications

Effective Separation and Simultaneous Determination of Four Fluoroquinolones in Milk by CE with SPE

Effective Separation and Simultaneous Determination of Four Fluoroquinolones in Milk by CE with SPE

Detection of Enrofloxacin and Its Metabolite Ciprofloxacin Using Gold Nanoparticles and Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Preconcentration and Determination of Ciprofloxacin with Solid-phase Microextraction and Silica-coated Magnetic Nanoparticles Modified with Salicylic Acid by UV-Vis Spectrophotometry

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