Determination of reducing sugars by oxidation in alkaline ferricyanide solution

Friedemann, Theodore E.; Weber, C. W.; Witt, Norman F.

doi:10.1016/0003-2697(62)90137-9

Cited by 24 publications

(7 citation statements)

References 12 publications

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“…The xylanase activity was assayed by measuring the amount of reducing sugars produced from beechwood xylan (Sigma) by the ferricyanide method (Friedemann et al 1962) using xylose as the standard. The reaction mixture consisted of 1% xylan in 75 mM of universal buffer, pH 7.0.…”

Section: Methodsmentioning

confidence: 99%

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

et al. 2017

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Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.

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Section: Methodsmentioning

confidence: 99%

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

et al. 2017

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“…The decorticated sal seeds were crushed and pelletized after steaming. The reducing sugars and starch were estimated according to the procedure described by Friedemann et al (9). The material was powdered in a hammer mill.…”

Section: Methodsmentioning

confidence: 99%

Detoxification of castor seed meal by interaction with sal seed meal

Gandhi

Cherian

Mulky

1994

J Americ Oil Chem Soc

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Castor (Ricinus communis) seed meal was detoxified by a novel method of wet mixing with sal (Shorea robusta)seed meal so that the toxic constituents of castor seed meal were neutralized by tannins, the toxicants present in the sal seed meal. The resulting product was innocuous, as revealed in the feeding studies in rats. The nutritional benefit of the treated material is improved by synergistic action of a protein such as casein. The aqueous extract of castor seed meal produced a smooth-muscle stimulant effect, whereas this effect was not observed with the extract from treated meal. This is a new concept of neutralization of two toxins by each other in two seed meals. The method can be employed to investigate the suitability of such a processed seed meal as an animal feedstuff ingredient.KEY WORDS: Allergen, castor seed meal, detoxification, ricin, ricinine, sal seed meal, tannin.Currently, India is the largest producer of castor seed ~icinus communis) in the world. The whole seeds yield

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“…Most reported spectrophotometric methods for determination of reducing sugars are based on their reactions with copper(II) 2,5,6 or with hexacyanoferrate(III). 10,12,13 According to Friedemann et al,14 spectrophotometric methodologies for reducing sugar determination based on oxidizing reactions with copper(II) or hexacyanoferrate(III) were first proposed in 1849 and 1859, respectively. A survey of scientific literature shows that analytical procedures based on both methodologies have been published in the last two decades.…”

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confidence: 99%

An Automated Multicommuted Flow Analysis Procedure for Photometric Determination of Reducing Sugars in Wine Employing a Directly Heated Flow‑Batch Device

Brasil¹,

Reis²

2017

J. Braz. Chem. Soc.

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This work presents an automated analytical procedure for the photometric determination of reducing sugars in wines. The procedure is based on the reaction of reducing sugars with potassium hexacyanoferrate(III) in an alkaline medium, and was implemented employing a multicommuted flow analysis (MCFA) approach. Sample heating to promote the reaction was accomplished using a flow-batch device, which was heated to 50 °C without the use of a fluid for heat exchange. The ability of the MCFA approach to handle small solution volumes was exploited in order to develop an analytical procedure that meets green analytical chemistry guidelines. After optimizing the operational variables, the analytical procedure afforded a linear response ranging from 0.75 to 6.00% (m/v) fructose (R 2 = 0.999), a limit of detection of 0.14% fructose (n = 11), a 3.5% relative standard deviation, reagent consumption and waste generation of 112 mg and 2.2 mL per determination, respectively, and an analytical throughput of 75 determinations per hour.

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Determination of reducing sugars by oxidation in alkaline ferricyanide solution

Cited by 24 publications

References 12 publications

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential

Detoxification of castor seed meal by interaction with sal seed meal

An Automated Multicommuted Flow Analysis Procedure for Photometric Determination of Reducing Sugars in Wine Employing a Directly Heated Flow‑Batch Device

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