Determination of Reactive Oxygen Species in Myringotomized Tympanic Membranes: Effect of Vitamin E Treatment

Polat, Şenol; Öztürk, Özmen; Üneri, Cüneyd; Yüksel, Meral; Haklar, Goncagül; Bozkurt, Süheyla; Küllü, Sevgi

doi:10.1097/00005537-200404000-00023

Cited by 24 publications

(41 citation statements)

References 38 publications

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“…Previous studies in literature have demonstrated that the use of vitamin E, ascorbic acid, selenium, N-acetylcysteine, L-carnitine can prevent the development of myringosclerosis [5,8,10,17] .…”

Section: Discussionmentioning

confidence: 98%

“…As a striking finding, as for the structure and location of plaques, microscopic examination of sclerotic lesions reveals no difference between rats and human beings [15,16] . Another issue to be underlined is that irreversible tissue damage induced by free oxygen radicals is the most important factor in the development of myringosclerosis [5,8,10,17] .…”

Section: Discussionmentioning

confidence: 99%

“…Besides, accumulation of calcium and phosphorus on this structure leads to crystalization and sclerosis [3,4] . Traumatic perforations or hyperoxidative condition and formation of free oxygen radicals are known to be effective on the development of sclerosis [5] . In many studies performed, various antioxidants have been tried to prevent the development of sclerosis after traumas or parasynthesis which injured TM and quantitative analysis of free oxygen radicals was perform to demonstrate a decrease in the development of sclerosis [5,6] .…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Systemic and Local Administration of Ascorbic Acid on Traumatic Perforation of Tympanic Membrane and Myringosclerosis

Güneş

Mutlu

Akın³

et al. 2015

Int Adv Otol

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OBJECTIVE:In the present study, tympanic membranes (TM) harvested from myringotomized rats were analyzed histopathologically to compare the systemic and local effects of ascorbic acid on the development of myringosclerosis. MATERIALS and METHODS:Forty male Wistar-Albino rats weighing between 350-400 g were included in this study. Under otomicroscopic examination, a standard 2-mm myringotomy incision was made on the posteroinferior quadrant of the TM of both ears. Rats were randomized into five groups as control, topical ascorbic acid 50 mg/kg, systemic ascorbic acid 50, 100, and 200 mg/kg groups, each group containing eight rats. On the 15th day of the study, the rats were decapitated, and bullas of the rats were extracted. Sections were stained with hematoxylin-eosin and examined through light microscopy. Inflammation, distribution width of plaques, edema, and neovascularization were observed on the lamina propria. Thickness of the TM was evaluated under the microscope and scored semiquantitatively. RESULTS:When intergroup comparisons of parameters related to total TM thickness were performed, differences between the control group and topical AA (ascorbic acid) or systemic treatment groups were found to be statistically significant (p<0.005). A statistically significant difference was detected among control, topical and systemic 200 mg/kg ascorbic acid groups for the edematous lamina propria (p=0.003 and p<0.05, respectively). CONCLUSION:For the total TM thickness, systemic and topical ascorbic acid use was effective when compared with the control group. It has been concluded that systemic use of higher doses of (200 mg/kg) ascorbic acid is beneficial in the resolution of the edematous lamina propria.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Impact of Systemic and Local Administration of Ascorbic Acid on Traumatic Perforation of Tympanic Membrane and Myringosclerosis

Güneş

Mutlu

Akın³

et al. 2015

Int Adv Otol

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“…Polat et al (22) concluded that inflammatory reactive oxygen species increased due to the immunological stimulation in the formation of tympanosclerosis after myringotomy and vitamin E reduced this effect. Selçuk et al (23) emphasized that the application of topical calcium channel blocker in guinea pigs on experimental myringosclerosis, which they created with myringotomy and Streptococcus pneumoniae type 3 inoculation, helped prevent tympanosclerosis.…”

Section: Discussionmentioning

confidence: 99%

A New Trend in the Management of Pediatric Deep Neck Abscess: Achievement of the Medical Treatment Alone

Üstündağ

Koçyiğit

Bulut

et al. 2017

Turk Arch Otorhinolaryngol

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Objective: We aimed to examine the effect of topical dexamethasone by otomicroscopic and histologic examinations for preventing myringosclerosis induced by myringotomy in rat tympanic membranes.Methods: Twenty-one Sprague Dawley rats (42 ears) were randomly divided into the following three groups after otomicroscopic examinations: experimental surgical group (5 rats), control group (8 rats), and study group (8 rats). The rats of all the groups underwent myringotomy in both tympanic membranes. Other than myringotomy, no additional procedure was performed for the rats in the experimental surgical group. In the control group, 0.9% NaCl was applied to the ears, whereas in the study groups, topical dexamethasone was applied to the ears. These applications in the control and study groups were repeated for nine days. On the 10th day of the study, the rat ears of all groups underwent otomicroscopic and histologic examinations. The prevalence and process of myringosclerosis were evaluated by otomicroscopic examination, whereas inflammation, membrane thickness, and myringosclerosis intensity were evaluated by histologic examination. Results:The growth of myringosclerosis with otomicroscopic examination was lesser in the study group in which topical dexamethasone was applied than the control and the experimental surgical groups. Moreover, it was observed that myringosclerosis effected fewer quadrants in the study group.Histologic examinations revealed that inflammation was significantly lesser in the study group than in the experimental surgical and control groups. The average membrane thickness values were significantly lesser in the study group than in the experimental surgical group. With respect to myringosclerosis growth, no statistically significant difference was observed among all groups, whereas with respect to myringosclerosis intensity, the rat ears in the study group were less severely affected.Conclusion: Thus, our study results suggest that applying topical dexamethasone after myringotomy has positive effects on limiting the intensity and prevalence of myringosclerosis.

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“…The conditions that damage the integrity of the middle ear, such as myringotomy, cause the oxygen concentration to rise to 21% by causing the ambient air to pass into the middle ear cavity [18] . Previous studies have shown that an increased oxygen concentration causes the development of myringosclerosis by increasing the production of oxygen free radicals [19,20] .…”

Section: Discussionmentioning

confidence: 99%

The Effect of Alpha-Lipoic Acid on Myringosclerosis in a Rat Model

Tatar¹,

Yörük²,

Ünal³

et al. 2015

Int Adv Otol

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OBJECTIVE: Alpha-lipoic acid (ALA) is an anti-inflammatory and antioxidant agent. In this study, the effects of ALA on the development of myringosclerosis in myringotomized rats were investigated. MATERIALS and METHODS:The rats were studied in five groups: each group included 6 rats (for a total of 30 rats). After a myringotomy was performed, the groups were administered a 25-mg/kg/day and 50-mg/kg/day dose of ALA for 15 days. The eardrums of the rats were examined histopathologically. We made the histopathological assessment from 0 to 3 on a 4-point scale by scoring the degree of the histopathological changes. In addition, superoxide dismutase, malondialdehyde, and glutathione peroxidase levels were measured in blood samples taken from the rats. RESULTS:In histopathological examinations, a high dose (50 mg/kg/day) of the ALA-applied group had histopathological results similar to those of the healthy group. In this group, we detected minimal collagen deposition, slight thickness in the lamina propria, and minimal epithelial disruption. In biochemical examinations, the maximum values of superoxide dismutase and glutathione peroxidase were observed in the group that received 50 mg/kg/day of ALA. CONCLUSION:In this study, the effect of ALA on myringosclerosis development was observed when a dose of 50 mg/kg/day was administered, and a recovery that showed a nearly intact tympanic membrane occurred at the end of 15 days of use. If an antioxidant were to be added to the treatment protocol of tympanic membrane defects, ALA may be a good candidate.

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Determination of Reactive Oxygen Species in Myringotomized Tympanic Membranes: Effect of Vitamin E Treatment

Cited by 24 publications

References 38 publications

The Impact of Systemic and Local Administration of Ascorbic Acid on Traumatic Perforation of Tympanic Membrane and Myringosclerosis

The Impact of Systemic and Local Administration of Ascorbic Acid on Traumatic Perforation of Tympanic Membrane and Myringosclerosis

A New Trend in the Management of Pediatric Deep Neck Abscess: Achievement of the Medical Treatment Alone

The Effect of Alpha-Lipoic Acid on Myringosclerosis in a Rat Model

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