Determination of Ranitidine in Human Plasma by SPE and ESI-LC-MS/MS for Use in Bioequivalence Studies

Abstract

A method for determining ranitidine in human plasma by ESI-LC-MS/MS was validated, using propranolol as internal standard. e extraction method used was solid phase extraction (SPE). Chromatographic separation was performed in a Chromolith C18 (50 mm × 4.6 mm i.d.) analytical column, which provided good separation of ranitidine and propranolol peaks with an analysis time of 2.5 minutes. Extraction yields of 94.4% for ranitidine and 89.4% for the internal standard were obtained. e lower limit of quanti�cation (LLO�) was 3.00 ng/mL, and limit of detection (LOD) was 0.05 ng/mL, with linearity ranging from 3.00 to 500 ng/mL. e results, thus, showed that this method is suitable for application in bioequivalence studies of ranitidine in human plasma.

Eco-Friendly Cloud Point Extraction Coupled With a Spectrophotometric Method for the Determination of Ranitidine Hydrochloride in Pharmaceutical Samples

“…CPE was considered environmentally friendly due to the use of a small amount of solvent and non-volatile surfactant and non-toxic. In addition to that in CPE the analyte can be determined and the matrix can be removed in one step (Jia et al, 2008 Bellorio et al, 2013).…”