Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection

Thermal stability of kudzu root (Pueraria Radix) isoflavones as additives to beef patties

“…Sibao et al (2007) reported puerarin, diadzin, genistein, daidzein and formononetin in the root of kudzu plant cultivated in China. Puerarin, diadzin, genistein and daidzein have been reported in kudzu root by other researchers (Zhang et al 2005;Prasain et al 2007;Chen et al 2001). In addition to the four isoflavones mentioned in the previous statement, Benlhabib et al (2004) found biochanin A in the kudzu root.…”

“…Kudzu (Pueraria lobata) native to Japan (called Kakkon) and China (called Ge Gen) has traditionally been used to relieve fever, promote salivation, relieve thirst, stop diarrhea, cure the common cold and relief wrist and shoulder stiffness (Chinese Pharmacopoeia Committee 2005;Sibao et al 2007). Kudzu root (Pueraria radix) has been studied and found to contain high amounts of isoflavones, puerarin, diadzin, daidzein, genistin, genistein, formononetin and their derivatives (Delmonte and Rader 2006;Wu et al 2011;Chen et al 2001;Benlhabib et al 2004). The kudzu root is widely used as a drug and highly valued for its medicinal properties (Fang et al 2005).…”

Analysis of natural antioxidants by capillary electromigration methods

“…As it occurs with other type of flavonoid compounds, isoflavones are suggested to be responsible of the antioxidant activity attributed to soy and other plant products. Isoflavones such as puerarin and daizein have been also analyze in traditional Chinese medicinal herbs using MEKC [36] and CZE methods [37]. Although flavonoids are weak acids, the basis of a hydrophobic separation (as that used in MEKC) has been considered more suitable for their separation.…”

“…Although flavonoids are weak acids, the basis of a hydrophobic separation (as that used in MEKC) has been considered more suitable for their separation. Several studies have been carried out to identify this kind of compounds by means of CE with electrochemical detection [36][37][38]. The use of this detector provides sensitivity enhancement compared to that obtained from an UV detector.…”

High-performance liquid chromatography–tandem mass spectrometry for identification of isoflavones and description of the biotransformation of kudzu root

“…Puerarin (daidzein 8-C-glucoside), daidzin (daidzein 7-Oglucoside), and daidzein (the structures shown in Fig. 2) are the main components; of these puerarin is present in the greatest amount [2]. Isoflavones are the main class of phytoestrogens, which have varied biological activity.…”