Determination of psilocybin in Psilocybe semilanceata using high-performance liquid chromatography on a silica column

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

“…Perkal et al (1980) found that homogenization of finely ground samples of Psilocybe subaeruginosa with 30 parts of methanol for no more than 2 minutes gave maximum yield of the alkaloids. Christiansen et al (1981a) found this method inadequate when analysing samples of Norwegian Psilocybe semilanceata. They extracted the samples twice with 10% 1 N ammonium nitrate in methanol in a centrifuge tube by rotating the tubes in a rotary mixer for 30 minutes.…”

“…The method of Christiansen et al 1981a with minor modifications. Jokiranta et al, 1984 Methanol, homogenization for 2 min., shaking for 16 h.…”

“…Normal phase chromatography on a silica column is the simplest form of this technique. It was used qualitatively by White (1979) and for quantitative analysis by Christiansen et al (1981aChristiansen et al ( , 1981b, Christiansen and Rasmussen (1983) and Jokiranta et al (1984). By this method Christiansen and Rasmussen obtained an excellent separation of the indole alkaloids present in Norwegian Psilocybe semilanceata.…”

“…P. Kumm., Norway, n=9 HPLC 5 500 -10 100 (traces) Christiansen et al, 1981a Psilocybe semilanceata (Fr.) P.…”

Thin-layer chromatography in the analysis of narcotic and strongly acting substances (a review)

“…This group includes, in particular, the drugs psilocine and psilocybine that have been placed under international control. Both these substances were later also found in Panaeolus conocybe and some other fungal cultures [18,19].…”