Determination of protein by its enhancement effect on the Rayleigh light scattering of carboxyarsenazo

Guang-wei, Yao

doi:10.1016/s0039-9140(99)00146-0

Cited by 34 publications

(14 citation statements)

References 15 publications

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“…The qualitative and quantitative analysis of proteins, especially the microdetermination of proteins, is one of the basic requisites in biochemical assays and clinical tests. Many methods have been proposed for the study of the binding reaction with protein such as spectrophotometry [1][2][3], fluorometry [4][5][6] and light scattering techniques [7][8][9][10]. The traditional methods are the Bradford method [11], the Lowry method [12] and bromocresol purple technique [13].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical studies of the reaction between albumin and amaranth and its analytical application

Sun

Jiao

Wang

et al. 2005

Journal of Electroanalytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Electrochemical studies of the reaction between albumin and amaranth and its analytical application

Sun

Jiao

Wang

et al. 2005

Journal of Electroanalytical Chemistry

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“…In this work, the scattering band (the RRS spectrum is corrected according to the reference. 29 ) is located at its absorption band, and the maximum scattering wavelength at 310 nm is close to the maximum absorption peak at 267 nm (Figure 6). Accordingly, this can lead to a resonance enhanced scattering.…”

Section: Influence On Rrs Spectramentioning

confidence: 85%

Absorption, Fluorescence and Resonance Rayleigh Scattering Spectra of Interaction of Papain with Calf Thymus DNA

Kong

Liu

et al. 2011

Chin. J. Chem.

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In weak acidic medium, interaction between papain and calf thymus DNA (ctDNA) resulted in absorption spectral change, fluorescence quenching of papain and remarkable enhancement of resonance Rayleigh scattering (RRS). The interaction types and binding modes were discussed by characteristics of RRS, absorption, fluorescence and circular dichroism spectra combining thermodynamic data. Four interaction types include electrostatic attraction, hydrophobic force, hydrogen bonding and aromatic stacking interaction. Papain interacted with the major groove of ctDNA. Aromatic stacking interaction is the main reason of change of absorption spectrum and fluorescence quenching of papain. Surface enhanced scattering effect, resonance energy transfer effect, increase of molecular volume and conformational change make contribution to RRS enhancement. The enhanced RRS intensity (∆I) is directly proportional to the concentration of ctDNA or papain. The detection limit (3σ) is 5.2 ng•mL -1 for ctDNA and 5.6 ng•mL -1 for papain. This creates conditions for determination of papain and ctDNA.

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“…Studies on the interaction of small molecules with protein can also be used for the understanding of the structures and functions of protein. 1 Many analytical methods had been proposed for protein investigation including UV-Vis absorption spectrophotometry, 2,3 fluorometry, 4,5 light scattering technique 6,7 and electroanalytical methods. 8,9 In recent years electrochemical methods had been greatly developed in bioanalytical chemistry with the advantages of low-cost, fast response and cheap instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

et al. 2009

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An electrochemical method for the determination of lysozyme (LYS) based on its interaction with alizarin red S (ARS) was established by linear sweep voltammetry in this paper. The electrochemical behaviour of ARS with LYS was investigated on a dropping mercury working electrode in 0⋅2 mol/L pH 4⋅8 Britton-Robinson (B-R) buffer solution. ARS showed a sensitive second order derivative linear sweep voltammetric reductive peak at -0⋅42 V (vs SCE). After the addition of LYS, the reductive peak current of ARS decreased without the shift of the reductive peak potential and no new waves appeared, which was due to the formation of a supramolecular complex of ARS with LYS in the solution. The stoichiometry of the ARS-LYS complex was further calculated by the electrochemical data with the results of the binding ratio as 3 : 1 and the binding constant as 2⋅82 × 10 14 . Under the selected conditions, the decrease of the second order derivative linear sweep voltammetric reductive peak current of ARS was in proportion to the LYS concentration in the range from 0⋅8 to 35⋅0 mg/L and the detection limit of LYS was calculated as 0⋅52 mg/L (3σ). Different kinds of LYS samples were detected satisfactorily with this method.

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Determination of protein by its enhancement effect on the Rayleigh light scattering of carboxyarsenazo

Cited by 34 publications

References 15 publications

Electrochemical studies of the reaction between albumin and amaranth and its analytical application

Electrochemical studies of the reaction between albumin and amaranth and its analytical application

Absorption, Fluorescence and Resonance Rayleigh Scattering Spectra of Interaction of Papain with Calf Thymus DNA

Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

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