Determination of proliferative fractions detected by immunohistochemistry with the monoclonal antibodies to bromodeoxyuridine and Ki‐67 in malignant lymphomas

Abstract

The stainability of proliferating cells with monoclonal antibodies to both bromodeoxyuridine (BrdU) and Ki-67 were compared in 22 malignant lymphomas and six related conditions. A significant correlation was observed (r = 0.78) between the positivities of proliferating cells determined using these two monoclonal antibodies. The growth fractions determined with the antibodies were usually related to the histologic classification, but labelling index was more varied in diffuse large cell type lymphoma. BrdU labelling was well correlated with the Working Formulation, and more useful as a prognostic indicator. On the other hand, Ki-67 was useful for broad recognition of cell proliferative ability.

Estimation of Growth Fraction in Fine Needle Aspirates from Non-Hodgkin’s Lymphoma Using Anti–Proliferating Cell Nuclear Antigen

“…9,11,13,14,17,19 Using flow cytometry and cell suspensions from paraffin-embedded tissue, some authors have demonstrated a correlation between the S-phase fraction and prognosis in NHL. 13,19 A correlation of these data with the Kiel classification showed that the S-phase fraction correlated strongly with Kiel grades, with a mean value of 7.3% for low grade lymphomas and 15.6% for high grade.…”

“…In addition, we correlated the proliferative activity and cytomorphologic subclassification of the lymphomas based on the updated Kiel classification. 26 Although recent reports have described a correlation between cell proliferation and lymphoma grading, 9,11,13,14,17,19 to our knowledge this is the first published report on a study using PCNA on FNA material.…”

“…16 Previous studies on non-Hodgkin's lymphomas (NHLs) using different methods have shown a strong correlation between indices of cell proliferation and histologic grading. 9,11,13,14,17,19 Comparison of PCNA data with those from flow cytometry 7 and Ki-67 labeling 14 indicates that PCNA provides an estimate of cell growth fraction in NHLs.…”

“…Several methods have been used to assess cell proliferation in tumors, including flow cytometry, 13,19 tritiated thymidine, 6,20 bromodeoxyuridine incorporation 17,24 and detection of cell cycle-related antigens by monoclonal antibodies. 4,7,10,15 Immunocytochemical methods have particular advantages over other techniques for the study of tumor proliferative activity, including simplicity, simultaneous morphologic correlation, rapidity of results and nonuse of radioactivity.…”

Quantitative study of KI‐67 antibody staining in 46 T‐cell malignant lymphomas using image analysis

“…These techniques include cell suspensions such as [3H]-thymidine (Silvestrini et al, 1989;Veneroni et al, 1988) or bromodeoxyuridine (Brd U) (Kawauchi et al, 1989;Sasaki et al, 1988;Schrape et al, 1987) labelling indices, or flow cytometric measurement of DNA content (Baisch and Gerdes, 1987;Bauer et af., 1986;Braylan and Benson, 1989;Christensson et al, 1989;Roos et al, 1985). They also concern histological sections such as counts of mitoses (Akerman et al, 1987;Donhijsen, 1987) or nucleolar organizer regions (Schrape et ai., 1987;Crocker and Nar, 1987;Hall et al, 1988a) or the counting of cells expressing a nuclear antigen present during the cell cycle (G 1, S, G2, M) as revealed by the monoclonal antibody Ki-67 (Veneroni et al, 1988;Schrape et al, 1987;Baisch and Gerdes, 1987;Hall et al, 1988a;Gerdes et al, 1983Gerdes et al, , 1984aGerdes et al, , b, 1987Hall et al, 1988b;Cibull et al, 1989;Drach et al, 1989;Schonk et al, 1989;Shirono et al, 1989;Weinberg, 1989;Weiss et al, 1987).…”