Determination of prednisolone, naphazoline, and phenylephrine in local pharmaceutical preparations by micellar electrokinetic chromatography

Abstract

A new, rapid, and simple method is described and used to resolve and quantify mixtures of prednisolone, naphazoline, and phenylephrine. The determination was accomplished by micellar electrokinetic chromatography (MEKC) using a fused-silica capillary (57 cm675 lm ID). The separation was carried out at 258C and 30 kV, using a 5 mM phosphate-5 mM borate buffer adjusted to pH = 8.2, 40 mM sodium dodecylsulfate (SDS) as background electrolyte. Under these conditions, the run time was 6.6 min and the limits of quantification were about 0.4 mg/L for every component. Repeatability and reproducibility studies showed no significant differences at 95% confidence level. Application of multivariate calibration regression spectrophotometric methods (PLS-1, PLS-2, and PCR) clearly demonstrated, especially in the case of PLS-1, the high resolving powder of these techniques if all possible interferences are suppressed. MEKC has been used for quantifying these compounds in different pharmaceutical products and the method gave good results compared with spectrophotometry. The pharmaceutical preparations do not require any separation steps when analysed by the two procedures described.

Use of multivariate analysis for optimization of separation parameters and prediction of migration time, resolution, and resolution per unit time in micellar electrokinetic chromatography

“…In a statistical term, PLS_1 attempts to maximize the covariance between the y-and x-dataset. The use of PCR and PLS_1 regression modeling for MEKC separation optimization has been previously demonstrated [20,37].…”

Development and validation of spectrophotometric method for phenylephrine hydrochloride estimation in nasal drops formulations

“…Various methods have been reported in the literature for the analysis of phenylephrine hydrochloride including spectrophotometry [4][5][6][7][8], spectrophotometry with chromogenic reagent [9], fluorometry [10], and chromatography [11,12]. High-performance liquid chromatography [13][14][15][16], micellar liquid chromatography [17], micellar electrokinetic chromatography [18], capillary zone electrophoresis [19,20], spectro-fluorimetric and derivative spectrophotometric methods [21], have also been reported for the determination of phenylephrine hydrochloride.…”

A fluorescence optosensor for analyzing naphazoline in pharmaceutical preparations

“…There are several analytical methods to determine NPZ; the majority are photometric [2,3] and chromatographic methods: thin layer chromatography [4], gas chromatography [5] and high performance liquid chromatography [6,7]. Capillary electrophoresis [8][9][10] and atomic absorption and emission [11] methods have also been published. NPZ presents intrinsic fluorescence and phosphorescence emission, thus different luminescence methods have been developed for its determination [12][13][14][15].…”