Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts

Puigventós, Lidia; Navarro, Meritxell; Alechaga, Élida; Núñez, Óscar; Saurina, Javier; Hernández-Cassou, Santiago; Puignou, L.

doi:10.1007/s00216-014-8298-2

Cited by 44 publications

(43 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination of the phenolic profile of pulp peach was performed by means of liquid chromatography-electrospraytandem mass spectrometry, as already described [17]. Twentysix selected compounds belonging to different phenolic classes (gallic acid, (+)-catechin hydrate, p-coumaric acid, psalicylic acid, caffeic acid, chlorogenic acid, (−)-epicatechin, (−)-epigallocatechin ethyl gallate, gallate, ferulic acid, fisetin, gentisic acid, homogentisic acid, polydatin, protocatechuic acid, protocatechualdehyde, quercetin dehydrate quercitrin hydrate, resveratrol, syringic acid, syringaldehyde, taxifolin, umbelliferon, sinapic acid, kaempferol, and vanillic acid) were quantified.…”

Section: High Performance Liquid Chromatography (Hplc) -Tandem Mass Smentioning

confidence: 99%

See 1 more Smart Citation

Prunus persica var. platycarpa (Tabacchiera Peach): Bioactive Compounds and Antioxidant Activity of Pulp, Peel and Seed Ethanolic Extracts

Loizzo

Pacetti

Lucci

et al. 2015

Plant Foods Hum Nutr

Self Cite

View full text Add to dashboard Cite

A comparative analysis of ethanol extracts from peel, pulp and seed of Prunus persica var. platycarpa (Tabacchiera peach) was done. The total phenol, flavonoid and carotenoid content as well as the antioxidant properties by using different in vitro assays (DPPH, ABTS, FRAP, Fe-chelating, β-carotene bleaching test) were evaluated. Pulp extract was subjected to liquid chromatography-electrospray-tandem mass spectrometry (HPLC-ESI-MS/MS). Gallic acid, protocatechuic acid, protocatechualdehyde, chlorogenic acid, p-coumaric acid, and ferulic acid were identified as main constituents. Pulp extract was characterized by the highest total phytonutrients content and exhibited the highest antioxidant activity in all in vitro assays (IC50 values of 2.2 μg/mL after 60 min of incubation by using β-carotene bleaching test and 2.9 μg/mL by using Fe-chelating assay). Overall, the obtained results suggest that P. persica var. platycarpa displays a good antioxidant activity and its consumption could be promoted. © 2015, Springer Science+Business Media New Yor

show abstract

Section: High Performance Liquid Chromatography (Hplc) -Tandem Mass Smentioning

confidence: 99%

“…The determination of the phenolic profile of pulp peach was performed by means of liquid chromatographyelectrospray-tandem mass spectrometry, as already described [17]. Briefly, the chromatographic separation was performed …”

Section: High Performance Liquid Chromatography (Hplc)-tandem Mass Spmentioning

confidence: 99%

Prunus persica var. platycarpa (Tabacchiera Peach): Bioactive Compounds and Antioxidant Activity of Pulp, Peel and Seed Ethanolic Extracts

Loizzo

Pacetti

Lucci

et al. 2015

Plant Foods Hum Nutr

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sample treatment was carried out following a previously described method [24]. Briefly, 0.1 g of sample were dispersed in 10 mL of acetone:H2O:HCl (70:29.9:0.1 v/v/v) and sonicated for 30 min.…”

Section: Samplesmentioning

confidence: 99%

HPLC Fingerprints for the Authentication of Cranberry-Based Products Based on Multivariate Calibration Approaches

Puigventós

Núñez

Saurina

2017

CAC

Self Cite

View full text Add to dashboard Cite

This work introduces the topic of the authentication of cranberry-based products and the detection and quantification of possible adulterations with other raw materials of lower quality. For such a purpose, genuine and adulterated cranberry samples were analyzed by reversed-phase HPLC with UV detection. Sample components were separated using an elution gradient based on 0.1% (v/v) formic acid aqueous solution and methanol as the components of the mobile phase. Chromatograms were recorded at 280, 370 and 520 nm. Data resulting from the injection of pure and adulterated samples, consisting of chromatographic fingerprints at each detection wavelength, were analyzed chemometrically. Preliminary studies by Principal Component Analysis showed that the sample extracts were clearly distributed depending on the extent of adulteration. Data was further treated by Partial Least Square regression to determine the percentages of grape contamination. It was found that even mixture samples containing low percentages of grape could be distinguished from genuine cranberry extracts. Besides, results obtained were highly satisfactory, with overall quantification errors lower than 5%. As a conclusion, the method proposed here resulted in an excellent approach to carry out the authentication of cranberry-based products relying on polyphenolic fingerprints.

show abstract

“…At present, there are several methods for the determination of polyphenolic and phenolic compounds, such as global assays for the evaluation of the total content by colorimetric or fluorimetric methods, or more specific ones, based on separation by capillary electrophoresis, liquid chromatography, and gas chromatography. However, liquid chromatography with UV or electrochemical detection, or that coupled with mass spectrometry is nowadays the technique of choice [17,[23][24][25][26][27][28][29][30][31][32]. However, the great chemical diversity among this family of compounds and the range of concentrations in which they can be found make liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) the most effective technique.…”

Section: Introductionmentioning

confidence: 99%

Targeted UHPLC–HRMS (Orbitrap) Polyphenolic and Capsaicinoid Profiling for the Chemometric Characterization and Classification of Paprika with Protected Designation of Origin (PDO) Attributes

et al. 2020

Self Cite

View full text Add to dashboard Cite

Society’s interest in the quality of food products with certain attributes has increased, the attribute of a Protected Designation of Origin (PDO) being an effective tool to guarantee the quality and geographical origin of a given food product. In Spain, two paprika production areas with PDO (La Vera and Murcia) are recognized. In the present work, targeted UHPLC-HRMS polyphenolic and capsaicinoid profiling through the TraceFinderTM screening software, using homemade accurate mass databases, was proposed as a source of chemical descriptors, to address the characterization, classification, and authentication of paprika. A total of 126 paprika samples from different production regions—Spain (La Vera PDO and Murcia PDO) and the Czech Republic, each including different flavor varieties, were analyzed. UHPLC-HRMS polyphenolic profiles showed to be good chemical descriptors to achieve paprika classification and authentication, based on the production region, through principal component analysis and partial least squares regression-discriminant analysis, with classification rates of 82%, 86%, and 100% for La Vera PDO, Murcia PDO, and the Czech Republic, respectively. In addition, a perfect classification was also accomplished among the flavor varieties for the Murcia PDO and Czech Republic samples. By employing the UHPLC-HRMS polyphenolic and capsaicinoid profiles as chemical descriptors, acceptable discrimination among La Vera PDO flavor varieties was also achieved.

show abstract

Determination of polyphenolic profiles by liquid chromatography-electrospray-tandem mass spectrometry for the authentication of fruit extracts

Abstract: 27Liquid chromatography-electrospray-tandem mass spectrometry MS/MS) was applied to the analysis and authentication of fruit-based products and fruit-

Cited by 44 publications

References 28 publications

Prunus persica var. platycarpa (Tabacchiera Peach): Bioactive Compounds and Antioxidant Activity of Pulp, Peel and Seed Ethanolic Extracts

Prunus persica var. platycarpa (Tabacchiera Peach): Bioactive Compounds and Antioxidant Activity of Pulp, Peel and Seed Ethanolic Extracts

HPLC Fingerprints for the Authentication of Cranberry-Based Products Based on Multivariate Calibration Approaches

Targeted UHPLC–HRMS (Orbitrap) Polyphenolic and Capsaicinoid Profiling for the Chemometric Characterization and Classification of Paprika with Protected Designation of Origin (PDO) Attributes

Contact Info

Product

Resources

About