Determination of polycyclic aromatic hydrocarbons in milk samples by high-performance liquid chromatography with fluorescence detection

Kishikawa, Naoya; Wada, Mitsuhiro; Kuroda, Naotaka; Akiyama, Syuzo; Nakashima, Kenichiro

doi:10.1016/s1570-0232(03)00066-7

Cited by 115 publications

(71 citation statements)

References 16 publications

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“…[1][2][3][4][5][6][7] Researches have been focused on the development of stationary phases capable of making good use of the p-p interaction between p electrons of a sample and a ligand, immobilized on a support, having p electrons. [8][9][10][11][12] There are commercially available columns of which separation mechanism is mainly based on the p-p interaction, such as PYE ® column, wherein 2-(1-pyrenyl)ethyl group is immobilized on silica gels.…”

mentioning

confidence: 99%

HPLC Retention Behavior of Poly-Aromatic-Hydrocarbons on Aminopropyl Silica Gels Modified with Cu(II)- and Ni(II)-Phthalocyanine Derivatives in Non-polar Eluent

Akizawa

Kitamura

Yamane

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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were purchased from Aldrich, U.S.A. Phthalocyanine (H 2 -PC) was obtained from Tokyo Kasei, Japan. As a supports, Develosil NH 2 -5 ® (hereinafter, referred to as "Dev", Nomura Kagaku, Japan) which is a spherical aminopropyl silica gels (particle size, 5 mm; specific surface area, 350 m 2 /g; average pore size, 120 Å) was used. As an eluent, n-hexane for HPLC (Nacalai Tesque, Japan) was mainly used, and as a solvent for packing M-PCS D , slurry solvent A Conc. (Chemco Scientific, Japan) was used. The commercially available PYE ® column and NPE ® (nitro-phenylethyl silica gels) column (Nacalai Tesque, Japan) were used for comparison to elucidate the underlying separation mechanism of the M-PCS D column. M-PCS D ColumnsMetal-phthalocyanine tetrasulfonyl chloride (MPCSCl: MϭH 2 , Ni 2ϩ and Cu 2ϩ , see Fig. 1) was synthesized from H 2 -PC, Cu-PC and Ni-PC as described by Nadler et al.17) The resultant Ni-PCSCl (5.6 mg), Cu-PCSCl (5.6 mg) or H 2 -PCSCl (5.3 mg) was each dissolved in dioxane (150 ml), following the addition of Dev (1.5 g), and was refluxed for 2 h. After standing to cool, M-PCS D was separated by filtration, and washed with methanol (100 ml), dried thoroughly in vacuo over diphosphorous pentoxide. The M-PCS D column was then prepared by packing the obtained M-PCS D into a stainless-steel column (f4.6ϫ150 mm) for HPLC by a conventional slurry packing method. Throughout the process, the filtrate and the like were clear and colorless, and the absorption spectra thereof were free from characteristic absorption band(s) of M-PCSCls or M-PCSs. From these evidences, it was concluded that M-PCS was completely immobilized on silica gels. The amount of M-PCS immobilized was also estimated from the absorption spectrum of the initial M-PCS solution in dioxane.

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confidence: 99%

HPLC Retention Behavior of Poly-Aromatic-Hydrocarbons on Aminopropyl Silica Gels Modified with Cu(II)- and Ni(II)-Phthalocyanine Derivatives in Non-polar Eluent

Akizawa

Kitamura

Yamane

et al. 2004

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…From these aspects, the determination method for PAHs in human milk was developed, 50) which involves a saponification with sodium hydroxide, liquid-liquid extraction with n-hexane and determination by HPLC with fluorescence detector. By the proposed method, 11 kinds of PAHs could be determined in human milk samples (Fig.…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Evaluation of Organic Environmental Pollutants Detected in Human Milk

Kishikawa

Kuroda

2009

JOURNAL OF HEALTH SCIENCE

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Human milk is an important food source for infant because it contains a large number of nutritional substances, growth and immune factors. However, human milk may be contaminated with environmental pollutants when mothers are exposed to these pollutants. In particular, lipophilic organic pollutants are likely to accumulate in milk fat. Therefore, the determination of the organic pollutants levels in human milk is necessary to estimate the health risks of these pollutants to milk-fed infants. For this purpose, a lot of reports for the measurements of environmental pollutants in milk samples have been published. In this review, we summarized the concentrations of harmful organic environmental pollutants such as polychlorinated organic compounds (PCOCs), polybrominated compounds, perfluorinated compounds (PFCs), polycyclic aromatic hydrocarbons (PAHs) and endocrine disrupting phenols in human milk samples. Also, we described the noteworthy results of several evaluative studies such as time trend and regional difference of pollutant levels.

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“…Entre os métodos utilizados para análise de HPAs em alimentos, a Cromatografia Líquida de Alta Eficiência tem sido amplamente utilizada em diferente matrizes [6,7,10,13,18,19].…”

Section: Determinação De Hpas Em Guaraná Em Pó Camargo Et Alunclassified

Determinação de hidrocarbonetos policíclicos aromáticos (HPAS) em guaraná em pó (Paullinia cupana)

Camargo¹,

Tfouni²,

Vitorino³

et al. 2006

Ciênc. Tecnol. Aliment.

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Determination of polycyclic aromatic hydrocarbons in milk samples by high-performance liquid chromatography with fluorescence detection

Cited by 115 publications

References 16 publications

HPLC Retention Behavior of Poly-Aromatic-Hydrocarbons on Aminopropyl Silica Gels Modified with Cu(II)- and Ni(II)-Phthalocyanine Derivatives in Non-polar Eluent

HPLC Retention Behavior of Poly-Aromatic-Hydrocarbons on Aminopropyl Silica Gels Modified with Cu(II)- and Ni(II)-Phthalocyanine Derivatives in Non-polar Eluent

Evaluation of Organic Environmental Pollutants Detected in Human Milk

Determinação de hidrocarbonetos policíclicos aromáticos (HPAS) em guaraná em pó (Paullinia cupana)

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