Determination of Plant Volatiles Using Solid Phase Microextraction GC–MS

Bramer, Scott Van; Goodrich, Katherine R.

doi:10.1021/ed5006807

Cited by 12 publications

(20 citation statements)

References 18 publications

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“…In previous studies [ 21 ], VOCs were collected from vines individually enclosed in bags using fibres coated with divinylbenzene (DVB), carboxen (CAR), and polydimethylsiloxane (PDMS). Solid-phase microextraction (SPME) has proved to be a useful tool to collect plant VOCs [ 22 ]. It uses a fused silica fibre coated with a polymeric material to adsorb and pre-concentrate volatile compounds.…”

Section: Resultsmentioning

confidence: 99%

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

et al. 2021

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Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine responses to elicitors are needed to determine the best conditions for their efficiency and position them in protection strategies. We previously reported that the elicitor sulphated laminarin induced the emission of volatile organic compounds (VOCs) by grapevine leaves. The present study was conducted to characterise and compare VOC emissions in response to other elicitors. Bastid® was first used to test the conditions of VOC collection and analysis. Using SBSE-GC-MS, we detected several VOCs, including the sesquiterpene α-farnesene, in a time-dependent manner. This was correlated with the induction of farnesene synthase gene expression, in parallel with stilbene synthesis (another defence response), and associated to resistance against downy mildew. The other elicitors (Redeli®, Romeo®, Bion®, chitosan, and an oligogalacturonide) induced VOC emission, but with qualitative and quantitative differences. VOC emission thus constitutes a response of grapevine to elicitors of various chemical structures. Therefore, VOC analysis is relevant for studying the impact of environmental factors on grapevine defence responses and optimising the performance of elicitors in vineyards.

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Section: Resultsmentioning

confidence: 99%

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

et al. 2021

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“…coated fibers with liquid and solid phases [4][5][6]8]. The SPME process comprises two steps: In the first, the fiber is exposed to the sample or its headspace and the target analytes partition from the sample matrix to the coating.…”

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confidence: 99%

Magnetic headspace adsorptive extraction of chlorobenzenes prior to thermal desorption gas chromatography-mass spectrometry

Vidal

Ahmadi

Fernández

et al. 2017

Analytica Chimica Acta

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This study presents a new, user-friendly, cost-effective and portable headspace solid-phase extraction technique based on graphene oxide decorated with iron oxide magnetic nanoparticles as sorbent, located on one end of a small neodymium magnet. Hence, the new headspace solid-phase extraction technique has been called Magnetic Headspace Adsorptive Extraction (Mag-HSAE). In order to assess Mag-HSAE technique applicability to model analytes, some chlorobenzenes were extracted from water samples prior to gas chromatography-mass spectrometry determination. A multivariate approach was employed to optimize the experimental parameters affecting Mag-HSAE. The method was evaluated under optimized extraction conditions (i.e., sample volume, 20 mL; extraction time, 30 min; sorbent amount, 10 mg; stirring speed, 1500 rpm, and ionic strength, non-significant), obtaining a linear response from 0.5 to 100 ng L for 1,3-DCB, 1,4-DCB, 1,2-DCB, 1,3,5-TCB, 1,2,4-TCB and 1,2,3-TCB; from 0.5 to 75 ng L for 1,2,4,5-TeCB, and PeCB; and from 1 to 75 ng L for 1,2,3,4-TeCB. The repeatability of the proposed method was evaluated at 10 ng L and 50 ng L spiking levels, and coefficients of variation ranged between 1.5 and 9.5% (n = 5). Limits of detection values were found between 93 and 301 pg L. Finally, tap, mineral and effluent water were selected as real water samples to assess method applicability. Relative recoveries varied between 86 and 110% showing negligible matrix effects.

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“…These organic compounds are formed by three isoprene (C 5 H 8 ) units and are synthesized by plants for protection against pathogens and environmental stress. 12 Piperine, the major piperamide of P. nigrum, was also putatively identified. The compound functions as an insecticide.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper (Piper nigrum L.) Waste: An Undergraduate Chemistry Experiment

et al. 2021

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A laboratory experiment was developed to introduce undergraduate chemistry students to the chemical characterization of agroindustrial waste extracts by gas chromatography−mass spectrometry (GC−MS). The students prepared hexane and dichloromethane extracts from black pepper (Piper nigrum L.) waste. GC−MS analysis revealed the possible presence of sesquiterpenes and piperine, compounds commonly found in P. nigrum fruits. During postlab discussion, the students were encouraged to search for information about the biological properties of the putatively identified compounds. Most students found that piperine shows larvicidal activity. Thus, an experiment was conducted by a postgraduate research group to investigate the larvicidal potential of P. nigrum waste extracts. From the results, the students concluded that the hexane extract had higher larvicidal activity than the dichloromethane extract. We also provided students with the opportunity to identify piperine using (+)-APCI FT-ICR MS spectra. The proposed experiment helped students to learn and practice GC−MS and stimulated them to search the scientific literature for solutions to environmental problems.

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Determination of Plant Volatiles Using Solid Phase Microextraction GC–MS

Cited by 12 publications

References 18 publications

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine

Magnetic headspace adsorptive extraction of chlorobenzenes prior to thermal desorption gas chromatography-mass spectrometry

Phytochemical Analysis and Determination of the Chemical Composition of Larvicidal Extracts of Black Pepper (Piper nigrum L.) Waste: An Undergraduate Chemistry Experiment

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