The aqueous extract of Ocimum basilicum leaves and zinc salts (zinc sulphate) was used as precursors in this investigation to create zinc oxide nanoparticles. ultraviolet-visible spectroscopy, scanning electron microscopy, Dynamic light scattering (DLS) were used to characterize the produced nanoparticles. (SEM), Energy-dispersive X-ray analysis (EDX), Because UV-Vis spectra exhibit absorption peaks in the 350 nm range. DLS analysis prove the nano size for the prepared colloidal of zinc oxide nanoparticles. SEM investigations showed a spherical shape with an average size of (30-80) nm. Analysis of (EDX) showed that elementary analysis was which are 31.8% and oxygen is 21.3%.