Determination of phenylephrine hydrochloride and Amoxicillin in binary mixture using Derivative Spectrophotometry methods

Abstract

A simple, precise and economical procedure for the simultaneous estimation of Phenylephrine Hydrochloride (PHE) and Amoxicillin (AMX) in formulation has been developed. The absorbance values of first derivative spectrum 228,258 nm andm 241 nm and second derivative spectrum 238, 277 nm and 226 nm was used for the estimation of PHE and AMX, respectively without mutual interference. This method obeyed beerand#39;s law in the concentration range mixing of 2-150mgL -1 PHE with 0,20,100 mgL -1 AMX and 2-240 mgL -1 AMX with 0,20,100 mgL -1 PHE and the second derivative depends on first derivative of the ratios spectra. The proposed methods are extensively validated. All the described methods can be readily utilized for analysis of pharmaceutical formulations.

Development of Spectrophotometric Method to Assay Phenylephrine Hydrochloride in Pure and in its Pharmaceutical Forms Using Diazotized Sulphanilic Acid

“…Other methods based on the oxidation of PPh either with an excess of bromosuccinamide (NBS) then the residual NBS was estimated by bleaching the color of methyl orange dye in acidic medium of HCl (1mL, 1M) (Zakaria, 2021) nor by adding an excess of chloramine-T and the residual was estimated by bleaching the color of indigo caramine dye (Battu et al, 2018) and PPh was also estimated using spectrophotometric method via flow injection analysis (Al-Abachi and Abed, 2015) and charge transfer complex formation with haematoxylin in alkaline medium (Ahmed and Amin, 2007). Several analytical techniques have also been published for PPh estimation, these techniques include: RP-HPLC-PDA method (Nagarjuna et al, 2020), high-performance thin layer chromatography (Ragab et al, 2019), chemometric-assisted spectrophotometry and RP-HPLC (Al-Shaalan, 2010), derivative spectrophotometric method (Aljeboree and Alshirifi, 2019), voltammetry (Yagmur, 2018), Conductometric titration method (Hasan et al, 2016). Finally, the dual wavelength method was used for the simultaneous estimated of PPh in bulk and pharmaceutical dosage forms (Suratiya and Pancholi, 2014).…”

Development and Validation of a RP-HPLC Method for Simultaneous Quantification of Paracetamol and Phenylephrine Hydrochloride

“…Given its clinical relevance, numerous researchers have endeavored to quantify phenylephrine concentrations using various analytical techniques. Among these, UV-visible spectroscopy [18][19][20][21], derivative spectroscopy [22][23][24], and high-performance liquid chromatography (HPLC) [25][26][27] have emerged as commonly employed methods for phenylephrine quantification. Each of these techniques offers distinct advantages in terms of sensitivity, specificity, and ease of implementation, catering to the diverse needs and preferences of researchers in different analytical settings.…”

Development of Two Simple Spectrophotometric Methods to Assay Phenylephrine-HCl as Pure Form And in Its Drug Forms

“…PPH is usually indicated to raise blood pressure in unstable patients with hypotension, especially causes from septic shock, and its nasal drops are used to treat symptoms such as itching of the nose and throat, sneezing and runny nose (2). PPH has been determined by using a wide variety of analytical techniques, these methods includes: RPhigh performance liquid chromatography (4,5,6), high-performance thin layer chromatography (7,8) and RP-HPLC-PDA method (9), voltammetry (10,11), conductimetric titration (12), flow injection analysis (13,14), ultraviolet spectrophotometry (15,16), renewable electrode of carbon nanotubes ceramic electrode (17) and derivative spectrophotometric method (18) have also been reported for the determination of PPH.…”