Determination of Phenolic Contents by HPLC, and Antioxidant, Antimicrobial, Antityrosinase, and Anticholinesterase Activities of Psephellus huber-morathii

Abstract

The goal of our study was to examine of antioxidant, antimicrobial, anticholinesterase activities, and phenolic composition of Psephellus huber-morathii. The antioxidant activities of extracts have been assessed by Ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging. Phenolic constituents were measured using reverse phase-high performance liquid chromatography (RP-HPLC), and Korkmaz et al.; JPRI, 26(1): 1-10, 2019; Article no.JPRI.47659 2 antimicrobial activity was investigated using the agar well diffusion method. Total phenolic content, FRAP, and CUPRAC results of aqueous extract have been better than methanolic extract, except for DPPH activity. Benzoic acid, and p-coumaric acid as major phenolic compounds were specified. Methanolic extract was especially effective against all microorganisms tested except for Yersinia pseudotuberculosis. The methanolic extract have been displayed inhibitory effect on tyrosinase. All extracts have been exhibited lower acetylcholinesterase, and butyrylcholinesterase inhibitory activities than galantamine. P. huber-morathii can be considered in the food, and drug industries due to antioxidant capacity and antimicrobial activities of the species. It can be potential source as anti-browning agents because of its average tyrosinase inhibitory activity. Original Research Article

Phenolic Content and Enzyme Inhibition Activities Barbarea auriculata var. paludosa, B. integrifolia and B. plantaginea (Brassicaceae)'s

“…Signals were identified using DAD detection at a column temperature of 25ºC. HPLC analyses were performed modified methods in our previous study [17,18].…”