Determination of phenolic compounds in honey using dispersive liquid–liquid microextraction

Campone, Luca; Piccinelli, Anna Lisa; Pagano, Imma; Carabetta, Sonia; Sanzo, Rosa Di; Russo, Mariateresa; Rastrelli, Luca

doi:10.1016/j.chroma.2014.01.081

Cited by 100 publications

(64 citation statements)

References 26 publications

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“…Thus, this data showed that the rain may have a beneficial effect on the formation and expression of this class of molecules. In addition, Campone et al (2014) argued that the polyphenols (flavonoids) of honey can be distinguished according to their origin. Some of them come from the nectar of plants visited by the bees, pollen, honeydew, or even the propolis.…”

Section: Discussionmentioning

confidence: 99%

Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

Azonwade¹,

Gbaguidi²,

̄so³

et al. 2018

Afr. J. Microbiol. Res.

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The aim of this study was to characterize polyphenolic compounds, antifungal and antioxidant activities of polyfloral honey collected from three different phytogeographical zones in Benin duing dry and rainy seasons. Spectrophotometrically at 517 nm, DPPH scavenging activity of tested samples was measured, while antifungal activity, total polyphenols, flavonoids and condensed tannins were evaluted by Dohou, Singleton and Zhishen methods, and a slightly modified method of Sun, respectively. Results revealed that only season has significant influence (P <0.05) on the flavonoids content and total phenols. The highest total phenols (781 ± 46 µgGAE/gs) and flavonoids (528 ± 31 µgCE/g) contents were obtained respectively from honey samples of the rainy and dry seasons. The samples collected in the rainy season in the three zones, have better antifungal activity than those collected in the dry season. The lowest IC 50 recorded was 27.58 ± 4.44 μg.µl -1 which showed that the highest antioxidant activity can be found in honey samples of the rainy season collected from the Sudanian zone. Overall, this study confirms that all investigated honey samples were good sources of polyphenolic compounds, and exhibit antifungal and antioxidant actvities.

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Section: Discussionmentioning

confidence: 99%

Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

Azonwade¹,

Gbaguidi²,

̄so³

et al. 2018

Afr. J. Microbiol. Res.

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“…Their isolation is highly desirable as they show positive effects on health maintenance and disease prevention similar to other phenolic compounds. Currently phenolic acids are isolated and characterized using different chromatographic methods including UHPLC and HPLC separations hyphenated to mass spectrometry, liquid-liquid extraction or solid-phase extraction [20][21][22][23]. However, the characterization of plant metabolites can be a complex procedure due to the presence of various compounds (sugars, chlorophyll, waxes, oils, etc.)…”

Section: Introductionmentioning

confidence: 99%

Poly(N-vinylimidazole/ethylene glycol dimethacrylate) for the purification and isolation of phenolic acids

Schemeth

Noël

Jakschitz

et al. 2015

Analytica Chimica Acta

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“…In this context, phenolic acids and flavonoids are considered as the most relevant antioxidant compounds present in honey [4][5][6][7]. The analysis of the volatile fraction together with polyphenols has been proposed for the determination of the botanic and/or geographic origins of honey [8,9].…”

Section: Introductionmentioning

confidence: 99%

Time-of-flight accurate mass spectrometry identification of quinoline alkaloids in honey

et al. 2015

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Time-of-flight accurate mass spectrometry (TOF-MS), following a previous chromatographic (gas or liquid chromatography) separation step, is applied to the identification and structural elucidation of quinoline-like alkaloids in honey. Both electron ionization (EI) MS and positive electrospray (ESI+) MS spectra afforded the molecular ions (M(.+) and M+H(+), respectively) of target compounds with mass errors below 5 mDa. Scan EI-MS and product ion scan ESI-MS/MS spectra permitted confirmation of the existence of a quinoline ring in the structures of the candidate compounds. Also, the observed fragmentation patterns were useful to discriminate between quinoline derivatives having the same empirical formula but different functionalities, such as aldoximes and amides. In the particular case of phenylquinolines, ESI-MS/MS spectra provided valuable clues regarding the position of the phenyl moiety attached to the quinoline ring. The aforementioned spectral information, combined with retention times matching, led to the identification of quinoline and five quinoline derivatives, substituted at carbon number 4, in honey samples. An isomer of phenyquinoline was also noticed; however, its exact structure could not be established. Liquid-liquid microextraction and gas chromatography (GC) TOF-MS were applied to the screening of the aforementioned compounds in a total of 62 honeys. Species displaying higher occurrence frequencies were 4-quinolinecarbonitrile, 4-quinolinecarboxaldehyde, 4-quinolinealdoxime, and the phenylquinoline isomer. The Pearson test revealed strong correlations among the first three compounds.

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Determination of phenolic compounds in honey using dispersive liquid–liquid microextraction

Cited by 100 publications

References 26 publications

Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

Poly(N-vinylimidazole/ethylene glycol dimethacrylate) for the purification and isolation of phenolic acids

Time-of-flight accurate mass spectrometry identification of quinoline alkaloids in honey

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