Polyphenolic profile, and antioxidant and antifungal activities of honey products in Benin

Abstract

The aim of this study was to characterize polyphenolic compounds, antifungal and antioxidant activities of polyfloral honey collected from three different phytogeographical zones in Benin duing dry and rainy seasons. Spectrophotometrically at 517 nm, DPPH scavenging activity of tested samples was measured, while antifungal activity, total polyphenols, flavonoids and condensed tannins were evaluted by Dohou, Singleton and Zhishen methods, and a slightly modified method of Sun, respectively. Results revealed that only season has significant influence (P <0.05) on the flavonoids content and total phenols. The highest total phenols (781 ± 46 µgGAE/gs) and flavonoids (528 ± 31 µgCE/g) contents were obtained respectively from honey samples of the rainy and dry seasons. The samples collected in the rainy season in the three zones, have better antifungal activity than those collected in the dry season. The lowest IC 50 recorded was 27.58 ± 4.44 μg.µl -1 which showed that the highest antioxidant activity can be found in honey samples of the rainy season collected from the Sudanian zone. Overall, this study confirms that all investigated honey samples were good sources of polyphenolic compounds, and exhibit antifungal and antioxidant actvities.

Chemical Composition, Nutritional Functions, and Antioxidant Activities of Honeys in Africa

“…In Burkina Faso honey, Meda et al (2005) and Beretta et al (2005) (Azonwade et al, 2018). Many kinds of honey produced in African regions exhibited higher antioxidant activities than those reported in the European Atlantic area (Escuredo et al, 2013).…”

“…The antifungal activity exhibited by the Ethiopian honey was suggested to be attributed to phytochemical, ascorbic acid and peroxidase. Similarly, Benin honey obtained from various sources were found to possess high antifungal activity against Aspergillus parasiticus (CMBB 20), Aspergillus ochraceus (CMBB 91), Aspergillus fumigatus (CMBB 89) and Aspergillus clavatus (NCPT 97) (Azonwade et al, 2018). More recently, Wasihun & Kasa (2016) have confirmed the antibacterial activity of red and white Ethiopian honey against multidrug-resistant pathogenic bacteria Staph.…”

“…From the above-mentioned results, the antioxidant activity of honey obviously does not only depend on the floral source but also on the geographical origin and the environments. Sudanese honey was distinctive with a high TPC (Mohammed & Babiker, 2010;Tahir et al, 2015) while Benin honey with TFC (Azonwade et al, 2018). Tab.…”

Studies on bioactivities of Manuka and regional varieties of honey for their potential use as natural antibiotic agents for infection control related to wound healing and in pharmaceutical formulations

“…Azonwade et al conducted an analysis and assessment of the antifungal properties of sixty kinds of honey samples harvested from 30 distinct phytogeographical locations in Benin–Sudanian, Sudano-Guinean, and Guinean–during the dry and wet seasons on four reference strains of Aspergillus including niger, parasiticus, fumigatus , and clavatus [55] . The experimental outcome concluded that though the antioxidant profiles restricted the honey samples to a dose dependency, all samples demonstrated clear antimycotic properties on all Aspergillus spp., with no particular distinctly sensitive strain.…”

Towards a Better Understanding of Nutritional and Therapeutic Effects of Honey and Their Applications in Apitherapy

“…Moreover, the variety of other substances contained in honey, namely phenolic compounds, play a key role in its antifungal effects [173]. These compounds have the ability to denature proteins and therefore the membranes of cells through the alteration of their stability [174]. It is well-known that the phenolic composition of each honey type depends on the botanical and geographical origin of collected plants, and thus various honey types can have a different outcome when it comes to antibacterial and antifungal properties [175,176].…”