Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC

Abstract

The important role of fruits in human health and nutrition has been better understood with the recent studies on biochemical contents of fruits having antioxidant properties. Being one of the similar studies, in this study, total antioxidant capacity (TAC), phenolic compound, organic acid, and vitamin C contents of three plum species (Prunus domestica L., Prunus cerasifera Ehrh., and Prunus spinosa L.) grown in Van locality (Turkey) were identified, and the correlation between the measured values was investigated. Phenolic compound, organic acid, and vitamin C contents were determined by high-performance liquid chromatography (HPLC) method. Analysis of phenolic compound indicated that chlorogenic acid was the predominant phenolic compound, and the highest value was measured in P. spinosa L. as 12.985 mg kg−1. Malic acid was the predominant organic acids and the highest value was measured in P. spinosa L. as 1.245 g 100 g−1. The highest TAC and vitamin C contents were also measured in P. spinosa L. as 1.021 mmol TE kg−1 and 25.492 mg 100 g−1, respectively. P. spinosa L. was found to be superior to the other two species with respect to antioxidant capacity and other biochemical contents. A significant (P ≤ 0.01) and positive correlation was reported between antioxidant capacity and vitamin C content.

Characterization of Phenolic Constituents from Prunus cerasifera Ldb Leaves

“…Taken together, the present study described the first time of isolation of the phenolic constituents from P. cerasifera Ldb leaves. Eleven phenolic acids including p-coumaric acid (1), caffeic acid (2), ferulic acid (3), chlorogenic acid (4), 3-Ocaffeoylquinic acid (5), 5-O-coumaroylquinic acid (6), 3-Ocaffeoylquinic acid methyl ester (7), chlorogenic acid methyl ester (8), 3-O-caffeoyl-5-O-coumaroylquinic acid or 3-Ocoumaroyl-5-O-caffeoylquinic acid (9), gallic acid (10), and protocatechuic acid (11) were identified. e current study pioneers to identify and report all the phenolic constituents from P. cerasifera Ldb leaves.…”

“…(vernacular name mirabalan) is native to the Tianshan area of the Xinjiang province and well liked by the community due to its higher nutrition values [1,3]. e genus Prunus have extensively been studied for their phytochemical screening and reported to contain different polyphenols and their derivatives [4][5][6][7][8][9][10][11]. Moreover, P. cerasifera is an endangered fruit species grown wild in Southern slopes of the Huicheng area in the Xianjiang province of China [12].…”

The Contents of Catechins And Anthocyanins in the Above-Ground Organs of Plants of Prunus Spinosa L.

“…According to our previous data on the biochemical properties of Prunus spinosa, the aboveground organs of this plant are rich in biologically active compounds. The content of cyanogenic glycoside prunasin in sprouts is in the range of 131-691 mg/100 g DM (Levon et al, 2007), catechin content in fresh fruits -78.5 mg/100 g DM (Mustafaeva, 2013), vitamin C -25.5 mg/100 g DM (Celik et al, 2017). Eight flavonoids were isolated from the flowers of Prunus spinosa: kaempferol, quercetin, kaempferol 3-O-alpha-L-arabinofuranoside, quercetin 3-O-alpha-L-arabinofuranoside, kaempferol 3-O-alpha-L-ramnopyranoside, kaempferol 7-O-alpha-L-ramnopyranoside, kaempferol 3-O-beta-D-xylopyranoside, kaempferol 3-O-(2''-E-p-coumaroyl)-alpha-L-arabinofuranoside.…”

Studies on the Phytochemical Composition and Antioxidant Activity of a Prunus spinosa L. Aqueous Extract

“…Also, in the Pinacho study, gallic acid was identified in the concentration of 0.2226 mg/100g and caffeic acid in the concentration of 0.1272 mg/100g [17]. The analysis of polyphenolic compounds by Celik et al showed the presence of chlorogenic acid at 1.2985 mg/100g [23]. The differences between polyphenol concentrations mentioned above may be linked to the pedoclimatic conditions.…”