Determination of oxolinic acid in faeces and urine of turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection

Pouliquen, Hervé; Armand, F.

doi:10.1016/s0378-4347(00)00394-7

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…Peaks were more symmetrical by using acetonitrile than methanol and the presence of tetrahydrofuran in the mobile phase did not reduce peak tailing and did not increase the theoretical plate counts of the cartridge (Pouliquen and Armand 2000).…”

Section: Chromatographic Conditionsmentioning

confidence: 95%

“…HPLC reversed-phase s have often been used for determining quinolone antibiotics in ®sh tissues and gave good performance (Ikai et al 1989, Plakas et al 1999, Pouliquen and Armand 2000.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

“…Orthophosphoric acid was used as aqueous eluent to make use of the increased speci®city and sensitivity given by the¯uorescence detection and to improve the peak shape by masking the residual silanol groups of the stationary phase (Pouliquen and Armand 2000).…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

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Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Pouliquen¹,

Morvan²

2002

Food Additives and Contaminants

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A procedure for the determination of residues of oxolinic acid (OA) and flumequine (FLU) in freeze-dried salmon muscle with attached skin, using reversed-phase high-performance liquid chromatography, is described. OA and FLU were extracted by a solid-liquid extraction procedure: after addition of hydrochloric acid, extraction used successively ethyl acetate, sodium hydroxide and chloroform. Liquid chromatography was performed on a 5 microm PuroSpher RP-18E cartridge using acetonitrile and 0.02 M aqueous orthophosphoric acid solution as mobile phase, with fluorescence detection. The performance of the method was established by spiking tissues with OA and FLU before the freeze-drying step. The method was linear over the concentration range 50-2000 ng/g freeze-dried tissue. Limits of detection and quantitation were 3.2 and 16 ng/g wet weight tissue respectively both for OA and FLU. Mean extraction recoveries of OA and FLU from freeze-dried tissue were 85.5 and 85.2% respectively. The method is suitable as a regulatory one for determination of residues of OA and FLU in freeze-dried salmon tissue.

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Section: Chromatographic Conditionsmentioning

confidence: 95%

“…HPLC reversed-phase s have often been used for determining quinolone antibiotics in ®sh tissues and gave good performance (Ikai et al 1989, Plakas et al 1999, Pouliquen and Armand 2000.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

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Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Pouliquen¹,

Morvan²

2002

Food Additives and Contaminants

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“…Oxolinic acid was quantified by high-performance liquid chromatography (HPLC) in intestinal contents, sediments, water and bivalves, according to the methods developed and validated in our laboratory (Pouliquen et al, 1994;Pouliquen & Armand, 2000).…”

Section: Oxolinic Acid Quantificationmentioning

confidence: 99%

Survey of antibiotic resistance in an integrated marine aquaculture system under oxolinic acid treatment

Giraud

Douet

Bris

et al. 2006

FEMS Microbiology Ecology

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The consequences of antibiotic use in aquatic integrated systems, which are based on trophic interactions between different cultured organisms and physical continuity through water, need to be examined. In this study, fish reared in a prototype marine integrated system were given an oxolinic acid treatment, during and after which the level of resistance to this quinolone antibiotic was monitored among vibrio populations from the digestive tracts of treated fish, co-cultured bivalves and sediments that were isolated on thiosulfate-citrate-bile-sucrose. Oxolinic acid minimum inhibitory concentration distributions obtained from replica plating of thiosulfate-citrate-bile-sucrose plates indicated that a selection towards oxolinic acid resistance had occurred in the intestines of fish under treatment. In contrast, and despite oxolinic acid concentrations higher than minimum inhibitory concentrations of susceptible bacteria, no clear evolution of resistance levels was detected either in bivalves or in sediments.

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“…Because it could induce psychopharmacological effects such as nervous excitation, stereotyped behavior, and insomnia, the assay methods for OA residues were widely studied. The reported methods are as following: HPLC-FL (Yorke and Froc 2000;Cohen et al 1999;Pouliquen and Armand 2000;Tyrpenou and Rigos 2004), HPLC-UV (Tourake et al 2001;Loussouarn et al 1997), capillary electraphoresis (Fierens et al 2000), and spectrophotometry (Dorota and Hanna 2003), etc. In all these methods, the spectrophotometric determination methods were extensively employed.…”

Section: Introductionmentioning

confidence: 99%

Study on the Fluorescence System of Oxolinic Acid‐Tb(III) and the Determination of Oxolinic Acid

Yang

Ran

et al. 2005

Analytical Letters

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In Tris-HCl buffer (pH ¼ 7.43), Tb 3þ can react with oxolinic acid (OA) and form a 1:2 complex, which emits the intrinsic fluorescence of Tb 3þ . Based on this, a new fluorimetric method of determination of OA is developed. Under the optimum conditions, the enhanced fluorescence intensity of the system is proportional to the concentration of OA in the range of 1.5 Â 10 27 2.5 Â 10 25 mol/L, and the detection limit is 5.5 Â 10 29 mol/L. Recovery test was also satisfactory. The experiments indicated that the luminescence mechanism was attributed to the M Ã -M luminescence.

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Determination of oxolinic acid in faeces and urine of turbot (Scophthalmus maximus) by high-performance liquid chromatography using fluorescence detection

Cited by 6 publications

References 16 publications

Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Determination of residues of oxolinic acid and flumequine in freeze-dried salmon muscle and skin by HPLC with fluorescence detection

Survey of antibiotic resistance in an integrated marine aquaculture system under oxolinic acid treatment

Study on the Fluorescence System of Oxolinic Acid‐Tb(III) and the Determination of Oxolinic Acid

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