Study on the Fluorescence System of Oxolinic Acid‐Tb(III) and the Determination of Oxolinic Acid

Abstract

In Tris-HCl buffer (pH ¼ 7.43), Tb 3þ can react with oxolinic acid (OA) and form a 1:2 complex, which emits the intrinsic fluorescence of Tb 3þ . Based on this, a new fluorimetric method of determination of OA is developed. Under the optimum conditions, the enhanced fluorescence intensity of the system is proportional to the concentration of OA in the range of 1.5 Â 10 27 2.5 Â 10 25 mol/L, and the detection limit is 5.5 Â 10 29 mol/L. Recovery test was also satisfactory. The experiments indicated that the luminescence mechanism was attributed to the M Ã -M luminescence.

Determination of Fluoroquinolones in Milk Samples by Postcolumn Derivatization Liquid Chromatography with Luminescence Detection

“…In addition, some potential interferences from sample matrix, which usually emit at shorter wavelengths, can be avoided. With regard to the determination of quinolone antibiotics, TSL has been proposed for the individual determination ( − ) or for the resolution of binary mixtures of quinolones (), the latter performed by using kinetic methodology. Most of these methods have been applied to the analysis of biological fluids ( 17 , − , their application to food analysis ( 18 , 23 , 24 ) being more limited to date.…”

“…With regard to the determination of quinolone antibiotics, TSL has been proposed for the individual determination ( − ) or for the resolution of binary mixtures of quinolones (), the latter performed by using kinetic methodology. Most of these methods have been applied to the analysis of biological fluids ( 17 , − , their application to food analysis ( 18 , 23 , 24 ) being more limited to date. The carboxylic and keto groups of quinolones are involved in the chelate formation, determining the intensity of the luminescent signal on the substituent attached to the nitrogen atom of the pyridone ring ().…”

Determination of albumins by its quenching effect on the fluorescence of Tb3+-oxolinic acid complex in presence of sodium dodecyl sulphate

“…Our previous studies [16] demonstrated that Tb 3+ -OA complex had a strong intrinsic fluorescence of Tb 3+ . In this paper, we found that its fluorescence could be quenched by albumins (bovine serum albumin (BSA), human serum albumin (HSA) or egg albumin (EA), especially in the presence of sodium dodecyl sulphate (SLS).…”

Oxolinic Acid Generated Green Fluorescence Based on a Terbium-Functionalized Covalent Organic Framework

“…Fluorescence sensing by signal amplification of fluorescence changes derived from the interaction of target with the corresponding fluorescent sensor is an effective analytic strategy. − It is of vital importance to design fluorescent sensors with outstanding performance for targets . Terbium (III, Tb 3+ )-based fluorescent sensors for OXO detection in environmental samples possess tempting potential due to its excellent optical characters, such as large stokes shifts, definite fluorescence emission bands, and prominent antenna effect. − However, annoying binding of free Tb 3+ by the potential interferences can cause the false-positive or false-negative signal response. , Besides, Tb 3+ is often of feeble fluorescence in the OXO-Tb 3+ complex since the solvent molecules can produce nonradiation quenching effect. All of these defects reduce the sensitivity of the free Tb 3+ -based fluorescent sensors.…”