Determination of organic carbon and nitrogen in marine sediments using the Carlo Erba NA-1500 analyzer

Verardo, David J.; Froelich, Philip N.; McIntyre, Andrew

doi:10.1016/0198-0149(90)90034-s

Cited by 734 publications

(365 citation statements)

References 3 publications

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“…Sediment samples were weighed into tin cups for nitrogen analysis. For the analysis of carbon, samples were weighed into aluminium cups and were subsequently sequentially acidified using sulphurous acid to remove any mineral carbonates (Verardo et al 1990). Samples were analysed for nitrogen and carbon contents, δ 15 N and δ 13 C, using a Carlo Erba NA1500 CNS analyser interfaced via a Conflo II to a Finnigan Mat Delta S isotope-ratio mass spectrometer operating in the continuous-flow mode.…”

Section: Methodsmentioning

confidence: 99%

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Cook¹,

Revill²,

Clementson³

et al. 2004

Mar. Ecol. Prog. Ser.

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The sources and cycling of organic matter on 2 mudflats in the Huon Estuary, SE Tasmania, were investigated over 4 seasons using pigment and lipid biomarkers as well as stable isotopes of carbon and nitrogen; 1 site was in the upper estuary,1 site was in a marine side-arm. The organic matter within the sediments at both sites was dominated by terrestrial sources, but most of this was refractory and only slowly remineralised. Significant, but seasonally variable, amounts of organic matter were derived from microphytobenthos (MPB), but this was rapidly remineralised and comprised only a minor fraction of the preserved sediment organic matter pool. Compound specific stable-isotope analysis of bacterial biomarkers suggested that the bacteria within the sediment were consuming microalgae-derived material. Separation of MPB from the sediment at the site in the upper estuary using the lens-tissue technique was found to significantly fractionate the algae community with cyanobacteria being enriched in the separated fraction. Nitrogen fixation probably contributed significantly to the growth requirements of cyanobacteria at the site in the upper estuary, as indicated by the low δ 15 N value of the cyanobacteria separated from the sediment.KEY WORDS: Microphytobenthos · Stable isotope · Compound-specific isotope-ratio mass spectrometry · Fatty acids · Sterols · Accessory pigment · Mudflat Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 280: [55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72] 2004 1999, Cahoon & Safi 2002), but this may shift seasonally to chlorophytes and cyanobacteria (Barranguet et al. 1997). In some areas, intertidal sediments may be dominated by cyanobacteria where high rates of nitrogen fixation may supply a significant amount of nitrogen to the benthic community (Stal et al. 1984, Bautista & Paerl 1985, Stal 1995.A number of approaches have been used to estimate the contributions of organic matter from various sources to sediments. Isotope ratios of 13 C/ 12 C have been used to estimate the relative contribution of terrestrial and marine sources to sedimentary organic matter (Fry & Sherr 1984). While this approach is relatively simple and gives an integrated estimate of sources for the total carbon in the sample, it will only provide useful information when there are 2 welldefined end-members. Furthermore, this technique gives little information about the type of marine or terrestrial organic matter in question.The use of lipid and pigment biomarkers such as fatty acids and sterols can allow the sources of various subfractions of organic matter to be identified much more specifically than any of the above approaches. However, unambiguously assigning sources to biomarkers is difficult, as they are rarely produced by a single class of organism. The advent of compound-specific stableisotope analysis has provided a powerful new tool for the differentiation between 2 sources of the same biomarker, provided the isotopic sign...

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Section: Methodsmentioning

confidence: 99%

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Cook¹,

Revill²,

Clementson³

et al. 2004

Mar. Ecol. Prog. Ser.

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“…Weighed samples were then acidified in-situ with 6% sulfurous acid to remove carbonate phases (Verardo, Froelich, & McIntyre, 1990). Each sediment sample was analyzed for total organic carbon (TOC) using a Carlo Erba NA-1500 elemental analyzer.…”

Section: Core Sampling and Total Organic Carbon (Toc) Analysismentioning

confidence: 99%

Field methods for amending marine sediment with activated carbon and assessing treatment effectiveness

Cho

Smithenry

Ghosh

et al. 2007

Marine Environmental Research

115

160

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“…Filters were folded, placed in plastic collection bags and stored at À 20 1C. POC and PN content of the suspended particulate matter on acid-fumed filters (Hedges and Stern, 1984) were determined at the University of California, Davis using a Carlo Erba NA-1500 Elemental Analyzer system (CE Elantech, Lakewood, NJ, USA) as described by (Verardo et al, 1990). For each day that samples were collected, we determined several basic physical characteristics of the Columbia River coastal margin.…”

Section: Methodsmentioning

confidence: 99%

Determining indicator taxa across spatial and seasonal gradients in the Columbia River coastal margin

et al. 2013

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Bacterioplankton communities are deeply diverse and highly variable across space and time, but several recent studies demonstrate repeatable and predictable patterns in this diversity. We expanded on previous studies by determining patterns of variability in both individual taxa and bacterial communities across coastal environmental gradients. We surveyed bacterioplankton diversity across the Columbia River coastal margin, USA, using amplicon pyrosequencing of 16S rRNA genes from 596 water samples collected from 2007 to 2010. Our results showed seasonal shifts and annual reassembly of bacterioplankton communities in the freshwater-influenced Columbia River, estuary, and plume, and identified indicator taxa, including species from freshwater SAR11, Oceanospirillales, and Flavobacteria groups, that characterize the changing seasonal conditions in these environments. In the river and estuary, Actinobacteria and Betaproteobacteria indicator taxa correlated strongly with seasonal fluctuations in particulate organic carbon (q ¼ À 0.664) and residence time (q ¼ 0.512), respectively. In contrast, seasonal change in communities was not detected in the coastal ocean and varied more with the spatial variability of environmental factors including temperature and dissolved oxygen. Indicator taxa of coastal ocean environments included SAR406 and SUP05 taxa from the deep ocean, and Prochlorococcus and SAR11 taxa from the upper water column. We found that in the Columbia River coastal margin, freshwater-influenced environments were consistent and predictable, whereas coastal ocean community variability was difficult to interpret due to complex physical conditions. This study moves beyond beta-diversity patterns to focus on the occurrence of specific taxa and lends insight into the potential ecological roles these taxa have in coastal ocean environments.

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Determination of organic carbon and nitrogen in marine sediments using the Carlo Erba NA-1500 analyzer

Cited by 734 publications

References 3 publications

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Carbon and nitrogen cycling on intertidal mudflats of a temperate Australian estuary. III. Sources of organic matter

Field methods for amending marine sediment with activated carbon and assessing treatment effectiveness

Determining indicator taxa across spatial and seasonal gradients in the Columbia River coastal margin

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