Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams

García, Pedro López; Santoro, María Inés Rocha Miritello; Singh, Anil Kumar; Kedor-Hackmann, Érika Rosa Maria

doi:10.1590/s1516-93322007000300008

Cited by 11 publications

(16 citation statements)

References 18 publications

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“…International Journal of Pharmacy and Pharmaceutical Sciences Several analytical methods have been reported for the determination of Hydroquinone in pure and pharmaceutical dosage forms using spectrophotometry [5][6][7][8], colorimetric reaction [9], FTIR [9] and capillary electrophoresis [10]. In the presence of treinoin, Hydroquinone has been determined by stability-indicating chromatographic methods [9,11] and spectrophotometric chemometric method [12].…”

Section: Fig 3: Tretinoinmentioning

confidence: 99%

Development and Validation of Analytical Spectrophotometric and Rp-HPLC Methods for the Simultaneous Estimation of Hydroquinone, Hydrocortisone and Tretinoin Ternary Mixture in Topical Formulation

Jamal

Gazy

2019

Int J Pharm Pharm Sci

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Objective: Development and validation of spectrophotometric and RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combination in pharmaceutical preparation. Methods: The proposed spectrophotometric method was able to determine TRT directly from its absorption spectrum at 362 nm, however, HQ and HC from their first derivative spectra at 284 nm and 252 nm, respectively, without any separation step. The RP-HPLC method was developed using a C18 Sunfire© waters column with a mobile phase composed of acetonitrile: phosphate buffer (adjusted to pH 6.1 using ortho-phosphoric acid) in the ratio of 30:70 %, v/v, respectively at a flow rate of 0.8 ml/min. Quantification was based on measuring peak areas at 260 nm. Results: The spectrophotometric method was able to selectively quantify each of HQ, HC and TRT in the ranges of 10-50 µg/ml, 2-10 µg/ml and 0.5-5 µg/ml, respectively. The RP-HPLC method was able to produce well-resolved peaks after 3.0, 8.2 and 20.2 min, in the ranges of 2-10 µg/ml, 0.1-1 µg/ml and 0.05-2 µg/ml, for HQ, HC and TRT, respectively. The obtained A, D1 or peak areas values plotted against the concentration of each of the three components showed linear response in the stated ranges. Both methods were validated in terms of linearity, LOD, LOQ, precision, accuracy and selectivity. Conclusion: Both developed proposed methods were applied for the determination of the active ingredients in the pharmaceutical formulation and the common excipients did not interfere in the analysis. The RP-HPLC method proved to be more sensitive when compared to the applied spectrophotometric method. However, the applied spectrophotometric methods, considered as green analytical chemistry, is a simple, time-saving method that requires minimal use of a hazardous solvent.

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Section: Fig 3: Tretinoinmentioning

confidence: 99%

Development and Validation of Analytical Spectrophotometric and Rp-HPLC Methods for the Simultaneous Estimation of Hydroquinone, Hydrocortisone and Tretinoin Ternary Mixture in Topical Formulation

Jamal

Gazy

2019

Int J Pharm Pharm Sci

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“…In majority, the determinations were below 2%, indicating high degree of agreement (repeatability) between experimental values. 17 Determination of repeatability can be done with a minimum of 6 times determination levels of 100%, or 9 times in the range of levels with 3 different levels, each repeated a number of 3 times. According to Ermer and Miller 10 it may be accepted if the repeatability RSD ≤ 2.00% of the test.…”

Section: Disccusionmentioning

confidence: 99%

“…This shows that the repeatability with RSD ≤ 2.00% of the test is acceptable. 17 The stability of all solutions and reagents is very important, whether in relation to temperature or in respect of time. 8 Comparison the stability of SPADNS reagent volume based on absorbance at λ 550 nm showed that the absorbance was stable, when the volume reached SPADNS reagent was added to the standard solution of 1 mL.…”

Section: Disccusionmentioning

confidence: 99%

Determination of fluoride content in toothpaste using spectrophotometry

Hastuti¹,

Lestari²,

Martono³

2013

Dent. J. (Majalah Kedokteran Gigi)

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“…Several techniques have been reported in the literature for estimation of HQ in cosmetics such as voltammetry [8, 9], high‐performance liquid chromatography (HPLC) [6], chemiluminescence (CL) [1, 10], flow injection analysis [11], capillary electrochromatography (CEC) [12] and spectrophotometry [13]. Among all the techniques, the last one is considered faster and simpler.…”

Section: Introductionmentioning

confidence: 99%

“…However, this technique has some limitations such as use of expensive and/or toxic ligands for complex formation, and hence its extensive use for HQ determination in aqueous samples [14–16]. The impracticality of spectrophotometry for HQ analysis in dilute samples of creams and cosmetics is attributable to its limited sensitivity and higher detection limits [13]. In this study, we report a highly sensitive, simpler, faster and economical spectrophotometric method for HQ determination in dilute organic matrices (skin lightening creams).…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive spectrometric method for determination of hydroquinone in skin lightening creams: application in cosmetics

Sirajuddin

Rauf

Kazi

et al. 2011

Intern J of Cosmetic Sci

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A highly sensitive, simpler, faster and economical UV/visible spectrophotometric method has been established for the estimation of hydroquinone (HQ) in dilute organic matrices. The method is based on using ammonium meta-vanadate as an oxidizing catalyst for conversion of HQ to p-benzoquinone (BQ) in the presence of oxygen. As a result of higher absorption of UV light by BQ than by HQ, its signal has been utilized for determining HQ at the trace level. The effect of various parameters such as amount of oxidizing agent, stability time, temperature, acids and bases, solvents and interference by various compounds has been studied upon the absorption of BQ as HQ. Under optimized conditions, Beer's Law was obeyed in the range of 0.025-2.00 μg ml(-1) HQ at 245.5 nm using 1 : 1 (V/V) 2-propanol/water system with a lower detection limit of 7 ng ml(-1) and linear regression coefficient of 0.9998. Relative standard deviation of 1.5% was observed for 0.5 μg ml(-1) HQ solution (n = 11). The newly developed method has been successfully applied to diluted samples of various skin lightening creams for free HQ determination at the trace level. Comparison of the results obtained by the proposed method with those by a previously reported method proved its validity.

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Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams

Cited by 11 publications

References 18 publications

Development and Validation of Analytical Spectrophotometric and Rp-HPLC Methods for the Simultaneous Estimation of Hydroquinone, Hydrocortisone and Tretinoin Ternary Mixture in Topical Formulation

Development and Validation of Analytical Spectrophotometric and Rp-HPLC Methods for the Simultaneous Estimation of Hydroquinone, Hydrocortisone and Tretinoin Ternary Mixture in Topical Formulation

Determination of fluoride content in toothpaste using spectrophotometry

Highly sensitive spectrometric method for determination of hydroquinone in skin lightening creams: application in cosmetics

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