Determination of oleanolic acid and ursolic acid contents inZiziphora clinopodioidesLam. by HPLC method

Abstract

A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of oleanolic acid and ursolic acid contents in the Ziziphora clinopodioides Lam. with short run time. Chromatographic separation is achieved by using HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18 column (150 × 4.6 mm, 10 μm, with pre-column), the mobile phase consists of methanol and 0.03 M phosphate buffer (pH = 3, 90:10). Detection wavelength is 214 nm. The speed of flow is 0.5 ml/min. The specimen handing quantity is 10 μl. The oleanolic acid's linearity range is 0.4 ~ 1.2 mg/ml (r = 0.9996). The ursolic acid's linearity range is 0.6 ~ 1.8 mg/ml (r = 0.9996), and the linear relationship is accurate. The average recovery (n = 6) of oleanolic acid is 99.5% (RSD = 1.19%) and ursolic acid is 102.3% (RSD = 1.25%). The content of oleanolic acid and ursolic acid in Ziziphora clinopodioides are 0.76 mg/g and 1.176 mg/g, respectively. The developed HPLC method can therefore be applied to both in vitro studies of oleanolic acid and ursolic acid formulations as well as drug estimation in biological samples.

Rapid extraction, isolation, and quantification of oleanolic acid fromLantana camaraL. Roots using microwave and HPLC-PDA techniques

“…Peaks were broad, and isomeric peak was not resolved. Further, manipulation in the mobile phase like methanol or ACN with phosphate buffer which suppresses the ionization of triterpenes [33], sharpens peak shapes and improves analytical sensitivity and resolution, but t R window was long. Because the separations were performed on a reversed-phase column, the most polar compound eluted earlier than OA, as expected.…”

Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18

“…[67] Up to date, only reverse phase liquid chromatography (RPLC) has widely been used to determinate them and proved to be the effective and convenient separation method. However, in the most common octadecyl silane (ODS) stationary phase system the resolution of OA and UA was unstable, ranging from 1.3 to 1.6 due to the sample composition was complex and the couple substances were position isomer;[891011] the low selectivity between OA and UA can be explained by similar log P (log of the octanol/water partition coefficient) values (6.468 and 6.427, respectively). [12] How to separate them simply was still a recognized problem.…”

Simultaneous determination of five marker compounds in Xuanfu Daizhe Tang by high-performance liquid chromatography coupled with diode array detection for quality control

“…Of these, single marker compound determination is simple, but it can not afford sufficient quantitative information for other active components in complex TCM. [3] Fingerprint analysis can evaluate the quality consistency and stability of herbal products, but cannot enable accurate quantification of analytes. [456] Many pure standards are required and suitable chromatographic conditions are difficult to optimize, but multi-constituent determination is widely used to control the quality of TCM[789] because of the advantage of simultaneous determination of many markers from different herbs for evaluation of total quality.…”