Determination of Olanzapine in rat brain using liquid chromatography with coulometric detection and a rapid solid-phase extraction procedure

Screening and quantification of antipsychotic drugs in human brain tissue by liquid chromatography–tandem mass spectrometry: Application to postmortem diagnostics of forensic interest

“…Beyond this time period that stability is not guaranteed because it was found that after 48 h the concentrations of olanzapine in the QCs were reduced to 95.3%. This result is in agreement with the findings of other authors [12,23], which reported that vitamin C can be added to the brain homogenate to prevent olanzapine degradation. For this reason, calibration brain eluates were freshly prepared and analyzed on the same day in this study,…”

“…The concentration of these drugs in brain samples (ng/g levels) has resulted in the need to develop more sensitive, robust and precise analytical methods. There are several methods based on GC [7,8], and LC methods that have been published for the determination of some of these antipsychotics in brain samples using different detection systems, including chlorpromazine and thioridazine using an electrochemical detector [9]; aripiprazole [10], haloperidol, olanzapine and clozapine [11] using ultraviolet detection; and olanzapine using coulometric detection [12].…”

Sensitive liquid chromatography/tandem mass spectrometry method for the simultaneous determination of olanzapine, risperidone, 9-hydroxyrisperidone, clozapine, haloperidol and ziprasidone in rat brain tissue

“…We found the storage of OLZ in rat brain homogenate at room temperature for 12 h produced significant degradation (18%) of OLZ. Saracino et al [16] reported that Vitamin C was added to the brain homogenate to prevent OLZ degradation. In this work, OLZ did not show significant degradation in rat brain homogenate kept at room temperature for up to 2 h without Vitamin C addition.…”

“…At present, the determination of some of these drugs in plasma has been established by high performance liquid chromatography (HPLC) with UV detection [6][7][8], coulometric detection [9], fluorescence detection [10] or electrochemical detection [11,12]. But, to date, very few papers have reported the determination of antipsychotic drugs in brain tissue [5,[13][14][15][16][17]. Most of these methods were not validated and did not deal with more than one agent.…”

Determinação de olanzapina em formulações farmacêuticas por espectrofotometria: desenvolvimento e validação

“…5,6 A olanzapina não é oficialmente registrada em nenhuma farmacopéia, 7 por isso torna-se necessário o desenvolvimento e a validação de um método analítico que seja capaz de quantificar o fármaco nas especialidades farmacêuticas comercializadas no mercado. Existem na literatura científica vários estudos visando a quantificação de olanzapina em amostras biológicas e formulações farmacêuticas, dentre os quais se destacam os métodos cromatográficos, 8,9 eletroforéticos, 10 eletroquími-cos, 10 espectrofotométricos, [10][11][12] injeção em fluxo, 1 cinéticos 13 e titulomé-tricos. 12 No entanto, a maioria destes métodos apresenta inconvenientes como, por exemplo, uso de equipamentos caros, 1,9,10 reagentes tóxicos 10,12 e procedimentos demasiadamente complexos 1,11 e demorados que os tornam pouco acessíveis e, em alguns casos, pouco viáveis.…”