Screening and quantification of antipsychotic drugs in human brain tissue by liquid chromatography–tandem mass spectrometry: Application to postmortem diagnostics of forensic interest

Sampedro, M. Carmen; Unceta, Nora; Gómez‐Caballero, Alberto; Callado, Luís F.; Morentín, Benito; Goicolea, M. Aránzazu; Meana, J. Javier; Barrio, Ramón J.

doi:10.1016/j.forsciint.2012.01.002

Cited by 42 publications

(24 citation statements)

References 37 publications

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“…In addition, αSMA positivity in extravascular regions was markedly reduced by haloperidol in the same lung sections (Figure 7, E-G). We performed LC-MS/MS analysis to confirm the availability of haloperidol in tissues using an established protocol (56,57) and found that it distributed preferentially to the lung compared with the heart, independent of the treatment with bleomycin, reaching a concentration that was one-tenth of that measured in cultured fibroblasts (Supplemental Figure 13).…”

Section: Resultsmentioning

confidence: 99%

High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation

Rehman¹,

Vodret²,

Braga³

et al. 2019

JCI Insight

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Section: Resultsmentioning

confidence: 99%

High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation

Rehman¹,

Vodret²,

Braga³

et al. 2019

JCI Insight

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“…Antidepressants and antipsychotics have been analyzed by gas chromatography (Papoutsis et al ., ), but mainly by HPLC, for example, with UV (Zhang et al ., , b) or fluorescence detection (Suckow et al ., ), which are cheaper but require a large volume of blood sample (0.5 mL; Aravagiri et al ., ). Today, detection is performed preferably by mass spectrometry, rendering effective confirmation of analytes and sufficient LOQs, both important in bioanalysis (Patil et al ., ; de Boer et al ., ; Ansermot et al ., ; Vecchione et al ., ; Sampedro et al ., ). Ionization is usually carried out in positive mode using electrospray ionization (ESI+) (Zhang et al ., ; Hasselstrom, ; Vecchione et al ., ; Liang and Bartlett, ; Ambavaram et al ., ) more often than atmospheric pressure chemical ionization (APCI+) (Aravagiri et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Sistik

Urinovska

Brozmanová

et al. 2015

Biomedical Chromatography

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A UPLC/MS/MS method with simple protein precipitation has been validated for the fast simultaneous analysis of agomelatine, asenapine, amisulpride, iloperidone, zotepine, melperone, ziprasidone, vilazodone, aripiprazole and its metabolite dehydro-aripiprazole in human serum. Alprenolol was applied as an internal standard. A BEH C18 (2.1 × 50 mm, 1.7 µm) column provided chromatographic separation of analytes using a binary mobile phase gradient (A, 2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v; B, 2 mmol/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v/v/v). Mass spectrometric detection was performed in the positive electrospray ionization mode and ion suppression owing to matrix effects was evaluated. The validation criteria were determined: linearity, precision, accuracy, recovery, limit of detection, limit of quantification, reproducibility and matrix effect. The concentration range was as follows: 0.25-1000 ng/mL for agomelatine; 0.25-100 ng/mL for asenapine and iloperidone; 2.5-1000 ng/mL for amisulpride, aripiprazole, vilazodone and zotepine; 2.3-924.6 ng/mL for dehydroaripiprazole; 2.2-878.4 ng/mL for melperone; and 2.2-883.5 ng/mL for ziprasidone. Limits of quantitation below a therapeutic reference range were achieved for all analytes. Intra-run precision of 0.4-5.5 %, inter-run precision of 0.6-8.2% and overall recovery of 87.9-114.1% were obtained. The validated method was successfully implemented into routine practice for therapeutic drug monitoring in our hospital.

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“…A number of methods have been developed for the determination of antipsychotics in biological samples . The sample preparation methods includes ionic liquid‐based emulsified microextraction accelerated by ultrasound radiation , microextraction by packed sorbent , extraction with a conical microcolumn packed with gamma‐methacryloxypropyltrimethoxysilane‐modified silica particles , disposable pipette extraction with reversed‐phase sorbent , SPE , LLE , protein precipitation , hybrid of SPE and precipitation , and so on. The quantification analysis was performed by HPLC , ultrafast LC–MS/MS , HPLC–MS/MS , UHPLC–MS/MS , GC , GC–MS/MS , and so on.…”

Section: Introductionmentioning

confidence: 99%

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

Zhong

Shen

Liu

et al. 2016

J of Separation Science

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An automatic on-line solid-phase extraction with ultra-high performance liquid chromatography and tandem mass spectrometry method was developed for the simultaneous determination of ten antipsychotics in human plasma. The plasma sample after filtration was injected directly into the system without any pretreatment. A Shim-pack MAYI-C8 (G) column was used as a solid-phase extraction column, and all the analytes were separated on a Shim-pack XR-ODS III column with a mobile phase consisting of 0.1% v/v formic acid in water with 5 mM ammonium acetate and acetonitrile. The method features were systematically investigated, including extraction conditions, desorption conditions, the equilibration solution, the valve switching time, and the dilution for column-head stacking. Under the optimized conditions, the whole analysis procedure took only 10 min. The limits of quantitation were in the range of 0.00321-2.75 μg/L and the recoveries ranged from 75.9 to 122%. Compared with the off-line ultra-high performance liquid chromatography and the reported methods, this validated on-line method showed significant advantages such as minimal pretreatment, shortest analysis time, and highest sensitivity. The results indicated that this automatic on-line method was rapid, sensitive, and reliable for the determination of antipsychotics in plasma and could be extended to other target analytes in biological samples.

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Screening and quantification of antipsychotic drugs in human brain tissue by liquid chromatography–tandem mass spectrometry: Application to postmortem diagnostics of forensic interest

Cited by 42 publications

References 37 publications

High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation

High-throughput screening discovers antifibrotic properties of haloperidol by hindering myofibroblast activation

Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Automatic on‐line solid‐phase extraction with ultra‐high performance liquid chromatography and tandem mass spectrometry for the determination of ten antipsychotics in human plasma

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