Determination of Niclosamide Residues in Rainbow Trout (Oncorhynchus mykiss) and Channel Catfish (Ictalurus punctatus) Fillet Tissue by High-Performance Liquid Chromatography

Abstract

Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combination with 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributary to the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed for the determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM during sea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow trout fillet tissue with a series of acetone extractions and cleaned up on C(18) solid-phase extraction cartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm for rainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04 microg/g was 77 +/- 6.5% and from channel catfish (n = 7) fortified at 0.02 microg/g was 113 +/- 11%. NIC detection limit was 0.0107 microg/g for rainbow trout and 0.0063 microg/g for catfish. Percent recovery of incurred radioactive residues by this method from catfish exposed to [(14)C]NIC was 89.3 +/- 4.1%. Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3) analyzed at 5 and 7.5 month periods were 78 +/- 5.1 and 68 +/- 2.4%, respectively. Percent recoveries of NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and 7.5 month periods were 88 +/- 13 and 76 +/- 21%, respectively.

Detection of the damage caused to DNA by niclosamide using an electrochemical DNA-biosensor

“…scopic methods (Schreier et al, 2000;Van Tonder et al, 1996;Daabees, 2000;Abdel-Fattah, 1997;Onur and Tekin, 1994;Emara, 1993;Sridevi and Reddy, 1991).…”

“…Analytical methods used for its quantification in natural water and in fish tissues employed HPLC (Schreier et al, 2000;Van Tonder et al, 1996) and spectrophotometric methods (Daabees, 2000;AbdelFattah, 1997;Onur and Tekin, 1994;Emara, 1993).…”

Uji Toksisitas Bahan Aktif Niklosamida Terhadap Crustacea Sebagai Water Treatment dalam Budidaya Udang Vannamei (Litopenaus vannamei)

“…yang merupakan hama dalam produksi padi. Sedangkan di Amerika, niklosamida biasa digunakan sebagai kontrol populasi ikan lamprey laut (Petromyzon marinus) pada aliran anak sungai danau Great (Schreier et al, 2000). Niklosamida dengan nama kimia benzoic acid, 2-[(3,5-dichloro-2-nitrophenyl)amino] dengan rumus empiris C 13 H 8 Cl 2 N 2 O 4 (Worthing, 1987) (Worthing, 1987).…”

TOKSISITAS LETAL MOLUSKISIDA NIKLOSAMIDA PADA BENIH IKAN MAS (Cyprinus carpio)

“…yang merupakan hama dalam produksi padi di Indonesia. Sedangkan di Amerika, niklosamida biasa digunakan sebagai kontrol populasi ikan lamprey laut (Petromyzon marinus) pada aliran anak sungai danau Great (Schreier et al, 2000). Keong mas merupakan hama yang dalam aktivitasnya memotong pangkal batang padi yang masih muda (Kurniawati et al, 2008).…”